| Technique(s) used for Glycosylation Detection | ESMS (electrospray mass spectrometry) giving broad heterogeneous molecular mass envelopes with a few discrete glycoform peaks |
| Technique(s) used for Glycosylated Residue(s) Detection | Liquid chromatography electron capture dissociation (ECD) tandem mass spectrometry (MS/MS). Also β-elimination reactions. |
| Protein Glycosylation- Implication | Glycosylation is required for flagellar filament assembly. |
| Glycan Information |
| Glycan Annotation | Two novel glycans - dimethylglyceric acid derivatives of pseudaminic acid (Pse5Ac7Ac) and 7-acetamidino pseudaminic acid are found. Ser181 and Ser207 were glycosylated by both glycans. Thr464 or Thr465 (or both, but exclusively) have been shown to be glycosylated by first glycan. Pse5Ac7Ac is also written as Pse5NAc7NAc (Pse). |
| BCSDB ID | 23773 24087 |
| Technique(s) used for Glycan Identification | MS/MS (tandem mass spectrometry), precursor ion scanning, and 1H, 13C NMR spectroscopy- HMBC (heteronuclear multiple bond correlation), COSY (correlation spectroscopy) |
| Protein Glycosylation linked (PGL) gene(s) |
| Characterized Accessory Gene(s) | Information currently not available with us. |
| Additional Comment | Sequon feature: Both S181 and S207 are located within the motif IS*TS pointing towards a probable consensus glycosylation sequence in FlaA.
Some enzymes (e.g., neuB2 gene product) have been implicated in the post translational modification of the flagellin protein (Ref. no. 4). |
| Literature |
| Year of Identification | 2001 |
| Year of Identification Month Wise | 2001.9.14 |
| Year of Validation | 2011 |
| Reference | Zampronio, C.G., Blackwell, G., Penn, C.W. and Cooper, H.J., 2011. Novel glycosylation sites localized in Campylobacter jejuni flagellin FlaA by liquid chromatography electron capture dissociation tandem mass spectrometry. Journal of proteome research, 10(3), pp.1238-1245. |
| Corresponding Author | Helen J. Cooper |
| Contact | School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, U.K. |
| Reference | Maita, N., Nyirenda, J., Igura, M., Kamishikiryo, J. and Kohda, D., 2010. Comparative Structural Biology of Eubacterial and Archaeal Oligosaccharyltransferases 2. Journal of Biological Chemistry, 285(7), pp.4941-4950. |
| Corresponding Author | Daisuke Kohda |
| Contact | Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan. |
| Reference | Logan, S.M., Hui, J.P., Vinogradov, E., Aubry, A.J., Melanson, J.E., Kelly, J.F., Nothaft, H. and Soo, E.C., 2009. Identification of novel carbohydrate modifications on Campylobacter jejuni 11168 flagellin using metabolomics‐based approaches. The FEBS Journal, 276(4), pp.1014-1023. |
| Corresponding Author | Evelyn C. Soo |
| Contact | NRC-Institute for Biological Sciences, Ottawa, Canada. |
| Reference | Thibault, P., Logan, S.M., Kelly, J.F., Brisson, J.R., Ewing, C.P. and Guerry, P., 2001. Identification of the carbohydrate moieties and glycosylation motifs in Campylobacter jejuni flagellin. Journal of Biological Chemistry, 276(37), pp.34862-34870. |
| Corresponding Author | Susan M Logan |
| Contact | Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario K1A 0R6, Canada. |
| Reference | Linton, D., Karlyshev, A.V., Hitchen, P.G., Morris, H.R., Dell, A., Gregson, N.A. and Wren, B.W., 2000. Multiple N‐acetyl neuraminic acid synthetase (neuB) genes in Campylobacter jejuni: identification and characterization of the gene involved in sialylation of lipo‐oligosaccharide. Molecular microbiology, 35(5), pp.1120-1134. |
| Corresponding Author | Brendan W. Wren |
| Contact | Department of Infectious and Tropical Diseases,London School of Hygiene and Tropical Medicine, LondonWC1E 7HT, UK |
