ProGP228 (Short glycopeptides)
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| ProGP ID | ProGP228 (Short glycopeptides) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Flavobacterium columnare (Flexibacter columnaris) ATCC 43622 |
| Domain | Bacteria |
| Classification | Phylum : Bacteroidetes Class : Flavobacteriia Orders : Flavobacteriales Family : Flavobacteriaceae Genus : Flavobacterium Species : columnare Strain : ATCC 43622 |
| Taxonomic ID (NCBI) | 996 |
| Genome Information | |
| EMBL | CP013992 |
| Organism Additional Information | Flavobacterium columnare is known all over the world as a pathogen of freshwater fish. It causes columnaris disease that is initiated as an infection of the body surface and gills leading to chronic, ulcerative condition. The disease often results in fish death. |
| Protein Information | |
| Protein Name | Short glycopeptides |
| UniProtKB Sequence | Val-Ala-Val-Asp-Ser-Ala-Ala (tripeptides to heptapeptides have been found with a minimal common fragment of Asp-Ser-Ala) |
| Glycosylation Status | |
| Glycosylation Type | O- (Ser) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | S5 |
| Experimentally Validated Glycosite(s ) in Mature Protein | S5 |
| Glycosite(s) Annotated Protein Sequence | VAVDS*(5)AA |
| Sequence Around Glycosites (21 AA) | VAVDAA |
| Technique(s) used for Glycosylation Detection | NMR spectra |
| Technique(s) used for Glycosylated Residue(s) Detection | ESI Q-TOF-MS (electrospray quadrupole time of flight mass spectrometry) and sequencing |
| Glycan Information | |
| Glycan Annotation | Linkage: Man-Ser. The glycan is an unusual hexasaccharide composed of 4-O-methyl-2-acetamido-2-deoxy-D-glucuronic acid (GlcNAcA), D-glucuronic acid (D-GlcA), 2,3-di-O-acetyl-D-xylose (D-Xyl), 2-O-methyl-D-glucuronic acid (D-GlcA), D-mannose (D-Man), and 2-O-methyl-L-rhamnose (L-Rha). |
| BCSDB ID | 5522 |
| Technique(s) used for Glycan Identification | NMR spectra and monosaccharide analysis by GC of alditol acetates. |
| Protein Glycosylation linked (PGL) gene(s) | |
| Additional Comment | The protein could not be identified as per the reference given. Only a heptapeptide was detected. |
| Literature | |
| Year of Identification | 2003 |
| Year of Identification Month Wise | 2003.11.14 |
| Year of Validation | 2003 |
| Reference | Vinogradov, E., Perry, M.B. and Kay, W.W., 2003. The structure of the glycopeptides from the fish pathogen Flavobacterium columnare. Carbohydrate research, 338(23), pp.2653-2658. |
| Corresponding Author | Evgeny Vinogradov |
| Contact | Institute for Biological Sciences, National Research Council, 100 Sussex Dr., Ottawa, ON, Canada K1A 0R6. evgeny. |