ProGP243 (Synthetic Glycopeptide)
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| ProGP ID | ProGP243 (Synthetic Glycopeptide) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Campylobacter jejuni NCTC 11168 |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : Epsilonproteobacteria Orders : Campylobacterales Family : Campylobacteraceae Genus : Campylobacter Species : jejuni Strain : NCTC 11168 |
| Taxonomic ID (NCBI) | 197 |
| Genome Information | |
| GenBank | AL111168.1 |
| EMBL | AL111168 |
| Organism Additional Information | Campylobacter jejuni is a microaerophilic, Gram-negative, human pathogen that is the major cause of bacterial food-borne diarrhoea (gastroenteritis). It is most frequently responsible for a form of post-infection neuromuscular paralysis known as Guillain Barre' syndrome. It also leads to an immunoproliferative small intestine disease that is a rare malignant lymphoma of the intestine. Motility is essential for pathogenicity. |
| Protein Information | |
| Protein Name | Synthetic Glycopeptide |
| UniProtKB Sequence | KDFNVSKA |
| Sequence length | 5 AA |
| Function | NA |
| Glycosylation Status | |
| Glycosylation Type | N- (Asn) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | N4 |
| Experimentally Validated Glycosite(s ) in Mature Protein | N4 |
| Glycosite(s) Annotated Protein Sequence | KDFN*(4)VSKA |
| Technique(s) used for Glycosylation Detection | In vitro chemo enzymatic glycosylation,HPLC analysis of glycopeptides with reference to known glycopeptide HPLC profiles |
| Technique(s) used for Glycosylated Residue(s) Detection | Not applicable |
| Protein Glycosylation- Implication | Not applicable |
| Glycan Information | |
| Glycan Annotation | GalNAc-α1,3-bacillosamine. |
| BCSDB ID | 20059 |
| GlyTouCan | G82182GL |
| Technique(s) used for Glycan Identification | NanoLC-MS/MS analysis and NMR spectroscopy-COSY (correlated spectroscopy), TOCSY (total correlation spectroscopy), NOESY (nuclear Overhauser effect spectroscopy) spectra, and 1H-13C HMBC (heteronuclear multiple bond coherence) spectra. |
| Protein Glycosylation linked (PGL) gene(s) | |
| OST Gene Name | PglB |
| Additional Comment | Engineered glycopeptide. In vitro characterization of the oligosaccharyl transferase activity of PglB protein from membrane fraction of Campylobacter jejuni using a synthetic disaccharide glycan donor (GalNAc-β-1,3-bacillosamine-pyrophosphate-undecaprenyl) and a peptide acceptor substrate (KDFNVSKA). |
| Literature | |
| Year of Identification | 2005 |
| Year of Identification Month Wise | 2005.4.29 |
| Year of Validation | 2005 |
| Reference | Glover, K.J., Weerapana, E., Numao, S. and Imperiali, B., 2005. Chemoenzymatic synthesis of glycopeptides with PglB, a bacterial oligosaccharyl transferase from Campylobacter jejuni. Chemistry & biology, 12(12), pp.1311-1316. |
| Corresponding Author | Barbara Imperiali |
| Contact | Department of Chemistry and Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA. |