ProGP256 (CmeC)
Home -> ProGPdb -> Search ProGP -> Display data
| ProGP ID | ProGP256 (CmeC) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Campylobacter jejuni NCTC 11168 |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : Epsilonproteobacteria Orders : Campylobacterales Family : Campylobacteraceae Genus : Campylobacter Species : jejuni Subspecies : jejuni Strain : NCTC 11168 |
| Taxonomic ID (NCBI) | 192222 |
| Genome Information | |
| GenBank | AL111168.1 |
| EMBL | AL111168 |
| Organism Additional Information | Campylobacter jejuni is a microaerophilic, Gram-negative, human pathogen that is the major cause of bacterial food-borne diarrhoea (gastroenteritis). It is most frequently responsible for a form of post-infection neuromuscular paralysis known as Guillain Barre' syndrome. It also leads to an immunoproliferative small intestine disease that is a rare malignant lymphoma of the intestine. Motility is essential for pathogenicity. |
| Gene Information | |
| Gene Name | Cj0365c (CmeABC operon) |
| NCBI Gene ID | 904688 |
| GenBank Gene Sequence | NC_002163.1 |
| Protein Information | |
| Protein Name | CmeC |
| UniProtKB/SwissProt ID | Q0PBE5 |
| NCBI RefSeq | YP_002343802.1 |
| EMBL-CDS | CAL34515.1 |
| UniProtKB Sequence | >tr|Q0PBE5|Q0PBE5_CAMJE Outer membrane channel protein CmeC (Multidrug efflux system CmeABC) OS=Campylobacter jejuni GN=cmeC PE=4 SV=1 MNKIISISAIASFTLLISACSLSPNLNIPEANYSIDNKLGALSWEKENNSSITKNWWKDF DDENLNKVVDLALKNNNDLKLAFIHMEQAAAQLGIDFSSLLPKFDGSASGSRAKTAINAP SNRTGEVSYGNDFKMGLNLSYEIDLWGKYRDTYRASKSGFKASEYDYEAARLSVISNTVQ TYFNLVNAYENENALKEAYKSAKEIYRINDEKFQVGAVGEYELAQARANLESMALQYNEA KLNKENYLKALKILTSNDLNDILYKNQSYQVFNLKEFDIPTGISSTILLQRPDIGSSLEK LTQQNYLVGVARTAFLPSLSLTGLLGFESGDLDTLVKGGSKTWNIGGNFTLPIFHWGEIY QNVNLAKLNKDEAFVNYQNTLITAFGEIRYALVARKTIRLQYDNAQASEQSYKRIYEIAK ERYDIGEMSLQDYLEARQNWLNAAVAFNNIKYSYANSIVDVIKAFGGGFEQSEDTSKNIK EESKNLDMSFRE |
| Sequence length | 492 AA |
| Subcellular Location | Outer membrane |
| Function | Outer membrane component of a multidrug efflux pump (RND superfamily) involved in antibiotic resistance. |
| Glycosylation Status | |
| Glycosylation Type | N- (Asn) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | N49 |
| Glycosite(s) Annotated Protein Sequence | >tr|Q0PBE5|Q0PBE5_CAMJE Outer membrane channel protein CmeC (Multidrug efflux system CmeABC) OS=Campylobacter jejuni GN=cmeC PE=4 SV=1 MNKIISISAIASFTLLISACSLSPNLNIPEANYSIDNKLGALSWEKENNSSITKN*(49)WWKDF DDENLNKVVDLALKNNNDLKLAFIHMEQAAAQLGIDFSSLLPKFDGSASGSRAKTAINAP SNRTGEVSYGNDFKMGLNLSYEIDLWGKYRDTYRASKSGFKASEYDYEAARLSVISNTVQ TYFNLVNAYENENALKEAYKSAKEIYRINDEKFQVGAVGEYELAQARANLESMALQYNEA KLNKENYLKALKILTSNDLNDILYKNQSYQVFNLKEFDIPTGISSTILLQRPDIGSSLEK LTQQNYLVGVARTAFLPSLSLTGLLGFESGDLDTLVKGGSKTWNIGGNFTLPIFHWGEIY QNVNLAKLNKDEAFVNYQNTLITAFGEIRYALVARKTIRLQYDNAQASEQSYKRIYEIAK ERYDIGEMSLQDYLEARQNWLNAAVAFNNIKYSYANSIVDVIKAFGGGFEQSEDTSKNIK EESKNLDMSFRE |
| Sequence Around Glycosites (21 AA) | LGALSWEKENNSSITKNWWKD |
| Technique(s) used for Glycosylation Detection | ZIC-HILIC enrichment |
| Technique(s) used for Glycosylated Residue(s) Detection | Reversed Phase LC-Tandem CID/HCD-MS and CID/ETD-MS |
| Glycan Information | |
| Glycan Annotation | Heptasaccharide GalNAc- α1,4-GalNAc- α1,4-(Glcβ1,3)-GalNAc- α1,4-GalNAc- α1,4-GalNAc- α1,3-Bac-β1 where Bac is bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucopyranose) |
| BCSDB ID | 20059 |
| GlyTouCan | G38348FF |
| Technique(s) used for Glycan Identification | Reversed Phase LC-Tandem CID/HCD-MS |
| Literature | |
| Year of Identification | 2007 |
| Year of Identification Month Wise | 2007.09 |
| Year of Validation | 2011 |
| Reference | Guerry, P., Ewing, C.P., Schoenhofen, I.C. and Logan, S.M., 2007. Protein glycosylation in Campylobacter jejuni: partial suppression of pglF by mutation of pseC. Journal of bacteriology, 189(18), pp.6731-6733. |
| Corresponding Author | Patricia Guerry |
| Contact | Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Ave., Silver Spring, MD 20910, USA |
| Reference | Scott, N.E., Parker, B.L., Connolly, A.M., Paulech, J., Edwards, A.V., Crossett, B., Falconer, L., Kolarich, D., Djordjevic, S.P., Højrup, P. and Packer, N.H., 2011. Simultaneous glycan-peptide characterization using hydrophilic interaction chromatography and parallel fragmentation by CID, higher energy collisional dissociation, and electron transfer dissociation MS applied to the N-linked glycoproteome of Campylobacter jejuni. Molecular & cellular proteomics, 10(2), pp.S1-S18. |
| Corresponding Author | Stuart J Cordwell |
| Contact | School of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia |