ProGP311 (BF2494)

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ProGP ID ProGP311 (BF2494)
Validation Status Characterized
Organism Information
Organism NameBacteroides fragilis (strain ATCC 25285 / NCTC 9343)
Domain Bacteria
Classification Phylum : Bacteroidetes
Class : Bacteroidia
Orders : Bacteroidales
Family : Bacteroidaceae
Genus : Bacteroides
Species : fragilis
Strain : strain ATCC 25285 / NCTC 9343
Taxonomic ID (NCBI) 272559
Genome Information
GenBank CR626927.1
EMBL CR626927
Organism Additional Information The Bacteroides constitute the major population of human intestinal microbiota. They are beneficial to the humans in terms of metabolism, development, and immunity. They play roles in recycling of bile acids, provision of short-chain fatty acids to the host and angiogenesis.
Gene Information
Gene NameBF2494
NCBI Gene ID 3287481
GenBank Gene Sequence NC_003228.3
Protein Information
Protein NameBF2494
UniProtKB/SwissProt ID Q5LCH1
NCBI RefSeq WP_010993031.1
EMBL-CDSCAH08194.1
UniProtKB Sequence >tr|Q5LCH1|Q5LCH1_BACFN Putative uncharacterized protein OS=Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) GN=BF2494 PE=4 SV=1 MVLLMAVSFAFAQEKNVKEAKSIAGEVKPDFAKAEQLINEALTNPETKDNAATWDVAGYI QKRINEKEMENAYLRKPYDTLKVYNSVLNMYNYYVKCDELAQIPNEKGKIKNKYRSANSK TILAERPNLINGGIQYFNLNKNEDALKYFAAYVDAATLPMMEKENLLEKDTILPQVAYYA TLAADRVGDKDAVMKYAQYALKDKENGQFAMQLLTDAYKAKGDTAKWVEKLQEGIVKFPE NQYFFANLVDYYSSSNQNDKAMQFADDMLAKDPNNKLYLYVKAYLYHNMKDYEKAIEFYK KTLDIDPAYAEACSNLGLVYLLQAQEYADKAPADINDPNYATAQAEIKKFYEAAKPYYEK ARELKPDQKDLWLQGLYRVYYNLNMGPEFEEIEKMM
Sequence length 396 AA
Subcellular LocationPeriplasm
Function Contains tetratricopeptide repeat (TPR) domains that mediate protein-protein interactions.
Glycosylation Status
Glycosylation Type O- (Thr) linked
Experimentally Validated Glycosite(s) in Full Length ProteinTranslation of the protein seems to occur seven codons upstream of the start codon (Met) with a predicted signal peptide (by LipoP) for periplasmic translocation. Therefore, in full length protein sequence the glycosylated residue position should be T87, T178 and T231.
Experimentally Validated Glycosite(s ) in Mature ProteinT80, T171, T224
Glycosite(s) Annotated Protein Sequence >tr|Q5LCH1|Q5LCH1_BACFN Putative uncharacterized protein OS=Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) GN=BF2494 PE=4 SV=1 MVLLMAVSFAFAQEKNVKEAKSIAGEVKPDFAKAEQLINEALTNPETKDNAATWDVAGYI QKRINEKEMENAYLRKPYDT*(80)LKVYNSVLNMYNYYVKCDELAQIPNEKGKIKNKYRSANSK TILAERPNLINGGIQYFNLNKNEDALKYFAAYVDAATLPMMEKENLLEKDT*(171)ILPQVAYYA TLAADRVGDKDAVMKYAQYALKDKENGQFAMQLLTDAYKAKGDT*(224)AKWVEKLQEGIVKFPE NQYFFANLVDYYSSSNQNDKAMQFADDMLAKDPNNKLYLYVKAYLYHNMKDYEKAIEFYK KTLDIDPAYAEACSNLGLVYLLQAQEYADKAPADINDPNYATAQAEIKKFYEAAKPYYEK ARELKPDQKDLWLQGLYRVYYNLNMGPEFEEIEKMM
Sequence Around Glycosites (21 AA) ENAYLRKPYDTLKVYNSVLNM
MEKENLLEKDTILPQVAYYAT
LTDAYKAKGDTAKWVEKLQEG
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Technique(s) used for Glycosylation DetectionMass shift detected on SDS-polyacrylamide gel, AAL (Aleuria aurantia lectin) reactivity, Pro-Q Emerald Glycostaining and reactivity towards anti-glycan antiserum
Technique(s) used for Glycosylated Residue(s) Detection Site-directed mutagenesis
Protein Glycosylation- Implication Protein glycosylation is central to the physiology of B. fragilis and is necessary for the organism to competitively colonize the mammalian intestine. Deletion of the lfg (protein glycosylation machinery) region results in a substantial growth deficiency in vitro and a complete inability to compete with wild-type bacteria in the mouse intestine.
Glycan Information
Glycan Annotation Exogenous fucose
Technique(s) used for Glycan Identification Lectin (AAL)binding
Protein Glycosylation linked (PGL) gene(s)
OST Gene NamePutative fucosyl transferase
Predicted Accessory Gene(s)BF4298-4306 region lfg (locus of fragilis glycosylation).
Additional CommentGlycosylation sequon features: the sequon has an aspartate (D) preceding the glycosylated T or S which is followed by an amino acid with one or more methyl groups (alanine, isoleucine, or leucine; (D)(S/T)(A/I/L/V/M/T). Moreover, none of the 17 unglycosylated S and T residues examined in of BF2494 (excluding two in the signal peptide) have a preceding D, although seven are followed by A, I, or L and one by V. Non methylated amino acids were not tolerated at third position of sequon in BF2494. Ile, Leu, and Val were found most frequently whereas Met is rarest at third position (reflecting the otherwise low number of Mets in proteins compared with the other five amino acids at the third position of the motif). The methyl group-containing amino acid at the third position being unreactive may play a role only in recognition of the site, whereas Asp residue may play a catalytic role.
Literature
Year of Identification2009
Year of Identification Month Wise2009.4.17
Year of Validation 2009
ReferenceFletcher, C.M., Coyne, M.J. and Comstock, L.E., 2011. Theoretical and experimental characterization of the scope of protein O-glycosylation in Bacteroides fragilis. Journal of Biological Chemistry, 286(5), pp.3219-3226.
Corresponding Author Laurie E. Comstock
ContactChanning Laboratory, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
ReferenceFletcher, C.M., Coyne, M.J., Villa, O.F., Chatzidaki-Livanis, M. and Comstock, L.E., 2009. A general O-glycosylation system important to the physiology of a major human intestinal symbiont. Cell, 137(2), pp.321-331.
Corresponding Author Laurie E. Comstock
ContactChanning Laboratory, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.