ProGP324 (FliC (Flagellin))

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ProGP ID ProGP324 (FliC (Flagellin))
Validation Status Characterized
Organism Information
Organism NameClostridium difficile BI-1
Domain Bacteria
Classification Phylum : Firmicutes
Class : Clostridia
Orders : Eubacteriales
Family : Peptostreptococcaceae
Genus : Clostridium
Species : difficile
Strain : Bl-1
Taxonomic ID (NCBI) 1496
Genome Information
GenBank GU048824
EMBL GU048824
Organism Additional Information Clostridium difficile is a gram-positive, anaerobic, spore-forming bacterium that produces TcdA and TcdB toxins causing severe tissue damage. It ia an opportunistic pathogen that is the major cause of antibiotic-associated diarrhoea and pseudomembranous colitis in humans. C. dificile infection can also result in abdominal pain, inflammation, and ulceration of the lining of the intestinal wall.
Gene Information
Gene NamefliC
Protein Information
Protein NameFliC (Flagellin)
UniProtKB/SwissProt ID D0V7J7
EMBL-CDSACY01338.1
UniProtKB Sequence >tr|D0V7J7|D0V7J7_CLODI Flagellin OS=Clostridium difficile GN=fliC PE=4 SV=1 MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAAGLAISEKMRAQIKGLD QAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETNTAEERQKIADELLQLKDE VERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTTNNNNEIKVALVNTSSIMSKAG ITSSTIASLNADGTSGTDAAKQMVSSLDVALKELNTSRAKLGAQQNRLESTQNNLNNTIE NVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLAQANQQPQGVLQLLG
Sequence length 290 AA
Subcellular LocationSurface
Function Forms the filaments of bacterial flagella.
Glycosylation Status
Glycosylation Type O- (Ser/Thr) linked
Experimentally Validated Glycosite(s) in Full Length ProteinT141, S174, S205
Experimentally Validated Glycosite(s ) in Mature ProteinT141, S174, S205
Glycosite(s) Annotated Protein Sequence >tr|D0V7J7|D0V7J7_CLODI Flagellin OS=Clostridium difficile GN=fliC PE=4 SV=1 MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAAGLAISEKMRAQIKGLD QAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETNTAEERQKIADELLQLKDE VERISSSIEFNGKKLLDGSST*(141)EIRLQVGANFGTNVAGTTNNNNEIKVALVNTSS*(174)IMSKAG ITSSTIASLNADGTSGTDAAKQMVS*(205)SLDVALKELNTSRAKLGAQQNRLESTQNNLNNTIE NVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLAQANQQPQGVLQLLG
Sequence Around Glycosites (21 AA) NGKKLLDGSSTEIRLQVGANF
EIKVALVNTSSIMSKAGITSS
SGTNAAKQMVSSLDVALKELN
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Technique(s) used for Glycosylation DetectionIntact mass analysis with QTOF2-MS (hybrid quadrupole time of flight mass spectrometry)
Technique(s) used for Glycosylated Residue(s) Detection Electron transfer dissociation (ETD) MS
Glycan Information
Glycan Annotation Several novel glycan moieties, ranging in mass from 494 Da to 917 Da. HexNAc and methylated deoxyhexose found.
Technique(s) used for Glycan Identification MS/MS (tandem mass spectrometry), nESI-feCID-MS/MS (nano-electrospray ionization-front-end collision-induced dissociation MS/MS) analyses
Protein Glycosylation linked (PGL) gene(s)
OST ProGT IDProGT29
Additional CommentFliC in C. difficile strain BI-1 is glycosylated in a similar manner.
Literature
Year of Identification2009
Year of Identification Month Wise2009.11.1
Year of Validation 2009
ReferenceTwine, S.M., Reid, C.W., Aubry, A., McMullin, D.R., Fulton, K.M., Austin, J. and Logan, S.M., 2009. Motility and flagellar glycosylation in Clostridium difficile. Journal of bacteriology, 191(22), pp.7050-7062.
Corresponding Author Susan M. Logan
ContactNRC-Institute for Biological Sciences, Ottawa, Canada.