ProGP421 (FlaB (Polar flagellin))
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| ProGP ID | ProGP421 (FlaB (Polar flagellin)) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Aeromonas hydrophila AH-3 (serotype O34) |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : gammaproteobacteria Orders : Aeromonadales Family : Aeromonadaceae Genus : Aeromonas Species : hydrophila Strain : AH-3 |
| Taxonomic ID (NCBI) | 644 |
| Genome Information | |
| GenBank | NZ_MTPO01000007.1. |
| EMBL | DQ860510 |
| Gene Information | |
| Gene Name | flaB |
| GenBank Gene Sequence | NZ_LYZG01000007.1 |
| Protein Information | |
| Protein Name | FlaB (Polar flagellin) |
| UniProtKB/SwissProt ID | C5E7P8 |
| NCBI RefSeq | WP_029301280.1 |
| UniProtKB Sequence | >tr|C5E7P8|C5E7P8_AERHY Flagellin OS=Aeromonas hydrophila GN=flaB PE=3 SV=1 MAMFINTNTSSLNAQRNLMNTTKSLDTSYTRLASGLRINSAKDDAAGLQISNRLTSQING LDQGNRNANDGISLAQTAEGAMDEVTGMLQRMRTLSQQSANGSNSAKDREALQKEVDQLG SEINRISRDTTFAGTKLLDGTYTGVFQVGADAQQTISFGLDQSMGFSISGIAKAAGTAVD MGTVIGSALDVTAIFASGSAGGISISTQSGAQNVLAAADLMLAVVDGKRAELGAVQNRLD STIRNQANISENVSAARSRIRDADFATETANMTKQNILQQAASSILAQANQRPQSALSLL G |
| Sequence length | 301 AA |
| Subcellular Location | Flagellum |
| Function | FlaB is one of the two monomers of polar flagellin which is constitutively expressed. |
| Glycosylation Status | |
| Glycosylation Type | O- (Thr) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | T161 |
| Glycosite(s) Annotated Protein Sequence | >tr|Q2N2Q5|Q2N2Q5_AERHY Flagellin OS=Aeromonas hydrophila GN=flaB PE=3 SV=1 MAMYINTNTSSLNAQRNLMNTNKSLDTSYTRLASGLRINTAKDDAAGLQISNRLTSQVNG LDQGNRNANDGISVAQTAEGAMDEVTSMLQRMRTLAQQSANGSNNTDDRTALQQEFDQLT TEINRISSDTSFGGQKLLDGNYKGSFQVGADAGQTITFKMT*(161)SAFTISGIASSTKGSATVA TSASGEPYTVTKGTTAVTSTSVSSIKAASSAQLAMANLDFMIKAVDSKRAELGAVQNRFD STIRNQSNVSENLSAARSRIRDADFATETANLTKQNILQQAASSILAQANQRPQSALSLL G |
| Sequence Around Glycosites (21 AA) | DAGQTITFKMTSAFTISGIAS |
| Protein Glycosylation- Implication | O-Glycosylation is necessary for flagellar biogenesis. Pseudaminic acid biosynthetic genes pseB and pseF homologues, were found to be required for flagellar formation and flagellin expression. |
| Glycan Information | |
| Glycan Annotation | A heptasaccharide with sequence 376 Da-Hex-Hex-HexNAc- HexNAc-HexNAc-102 Da, comprised of three N-acetylhexoasmines (with variable addition of 0-2 phosphate groups and 0-2 methyl groups on each), two hexoses and two unknown monosaccharides of 376 and 102 Da. The 376 Da residue is pseudaminic acid (Pse5Ac7Ac) modified by the addition of an acetyl group and water. The number of heptasaccharide chains may vary from one to six, reflecting glycan heterogeneity. |
| Literature | |
| Year of Identification | 2012 |
| Year of Identification Month Wise | 2012.8.10 |
| Year of Validation | 2012 |
| Reference | Wilhelms, M., Fulton, K.M., Twine, S.M., Tomás, J.M. and Merino, S., 2012. Differential glycosylation of polar and lateral flagellins in Aeromonas hydrophila AH-3. Journal of Biological Chemistry, 287(33), pp.27851-27862. |
| Corresponding Author | Juan M. Tomás |
| Contact | Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, Diagonal 645, 08071 Barcelona, Spain |