ProGP430 (Serine protease)

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ProGP ID ProGP430 (Serine protease)
Validation Status Characterized
Organism Information
Organism NameSulfolobus solfataricus P2 (DSM 1617)
Domain Archaea
Classification Phylum : Crenarchaeota
Class : Thermoprotei
Orders : sulfolobales
Family : Sulfolobaceae
Genus : Sulfolobus
Species : solfataricus
Strain : P2 (DSM 1617)
Taxonomic ID (NCBI) 273057
Genome Information
GenBank AE006641
EMBL AE006641.1
Gene Information
Gene NameSSO2551
NCBI Gene ID 27428851
GenBank Gene Sequence NZ_LT549890.1
Protein Information
Protein NameSerine protease
UniProtKB/SwissProt ID Q97VR2
NCBI RefSeq WP_009990123.1
EMBL-CDSAAK42680.1
UniProtKB Sequence >tr|Q97VR2|Q97VR2_SULSO Serine protease, subtilase family, putative OS=Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) GN=SSO2551 PE=4 SV=1 MESRIIQVVVISTFLVLSVLFPLLSLAYSTTSINPSYPQSNVISALPSNTNIILYFFIPP KNLNELYLIAQEVANHQIKPLSNAQLVSMFSNQDKVNESIKYLESKGFTIIYRSPFEIMA EAPVSLVSSVFETSFVLAKSTNGEIYYKPAGNVKIPSTLNNLLIGGLTNFTNVSLPLIQL GKLENGNLIPNKQAYSSFVYTFQFSATWYTPKVIEGAYNITPLLNSTADKKVTIAIIDAY GDPEIYQDVNLFDARFGLPPINLTVLPVGPYHPENGLFTGWFEEVALDVEAAHAAAPYSN ILLVVAPSATLEGLFSAIDVVVSEDLAQVVSMSWGLPGILFGASGFYAVFNGIIFPNYPY YDYYFELGSAEGITFLASSGDLGAYNDLPTVYGSANYPASSPFVTAVGGTSLFANITSGY ISTYNSTGNFGAEIAWSVNPLYFGVIQGGVSSGGGYSQLFPAPWYQRYVTHSNYRAIPDV AADANPYTGFTIYALGQEVVIGGTSLSAPLWAGIIADIDGIIGHPLGLVNPILYEIYQNT TLYHQAFHQISLGYNGYYYANSSYNLVTGLGSPNAGMLGVIIKHSLSKSLAISVSTFETG VFQPWYFYGSTFTIAAYITYPNNTIVSQGSFNAYIYTSEGYLATVPLSFNGSYWVGNYTI TPNNPPNLWEIVVNGSSDQFTGVGTVEVDVGESINIVSPIPYPYSFPIPYNSPFGIEAWI YYPNGTPVVNQSVTAYLVSNDGKLLASIPLTMMAPGLYEGSYALLPPLPQGTYLLIVNDS YGSAFSYVYFGEYNFGAILTPINDGFPAASPGQNITIIDEVLTPELTGLFTSNVTAYIYN QHGNLIDQVKLTPAPDEIQFGVYLLFFLYYANFTIPFDASPGFYNVVIQSISNTSTGLVK ADFITSFYVSPANLTLNVKVNNVVYEGELLKIFANITYPNGTPVKYGMFTATILPTSLNY EQLIIGFEAGIPLQYNSTLGEWVGIYSIPSIFYGSIFQGSSVYSLAGPWNVIVSGVSWNG YNLYSTPSSFNFVNVMPYTFINNIVVSSKSLDSPLLSKINSTTYMLSNVKSNNITINGMN VILSNVIANTVTVKNSNIMITSSTINQLVLDNSSVSIIGSKIGGDNIAVVANDSNVTIVS SVIQDSKYAFLQPNSVISLSGVNMYNVTSLSSIPAPRITYLSTTNVTTSKESIIVNITGE YLRLLGVSMNNKPVGYSVISSSPSSISLSIPFNASQLSDGQYIFTVSISDGLPYNLTFNL LNNYHLIIVQDHLKALQGSVNLLTVIAIISLIIAIIAVALLFVFTRRR
Sequence length 1308 AA
Subcellular LocationIntegral component of membrane
Function Serine-type endopeptidase activity and tripeptidyl-peptidase activity.
Glycosylation Status
Glycosylation Type N- (Asn) linked
Experimentally Validated Glycosite(s) in Full Length ProteinN262, N539, N976 and N1185
Glycosite(s) Annotated Protein Sequence >tr|Q97VR2|Q97VR2_SULSO Serine protease, subtilase family, putative OS=Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) GN=SSO2551 PE=4 SV=1 MESRIIQVVVISTFLVLSVLFPLLSLAYSTTSINPSYPQSNVISALPSNTNIILYFFIPP KNLNELYLIAQEVANHQIKPLSNAQLVSMFSNQDKVNESIKYLESKGFTIIYRSPFEIMA EAPVSLVSSVFETSFVLAKSTNGEIYYKPAGNVKIPSTLNNLLIGGLTNFTNVSLPLIQL GKLENGNLIPNKQAYSSFVYTFQFSATWYTPKVIEGAYNITPLLNSTADKKVTIAIIDAY GDPEIYQDVN*(262)LFDARFGLPPINLTVLPVGPYHPENGLFTGWFEEVALDVEAAHAAAPYSN ILLVVAPSATLEGLFSAIDVVVSEDLAQVVSMSWGLPGILFGASGFYAVFNGIIFPNYPY YDYYFELGSAEGITFLASSGDLGAYNDLPTVYGSANYPASSPFVTAVGGTSLFANITSGY ISTYNSTGNFGAEIAWSVNPLYFGVIQGGVSSGGGYSQLFPAPWYQRYVTHSNYRAIPDV AADANPYTGFTIYALGQEVVIGGTSLSAPLWAGIIADIDGIIGHPLGLVNPILYEIYQN*(539)T TLYHQAFHQISLGYNGYYYANSSYNLVTGLGSPNAGMLGVIIKHSLSKSLAISVSTFETG VFQPWYFYGSTFTIAAYITYPNNTIVSQGSFNAYIYTSEGYLATVPLSFNGSYWVGNYTI TPNNPPNLWEIVVNGSSDQFTGVGTVEVDVGESINIVSPIPYPYSFPIPYNSPFGIEAWI YYPNGTPVVNQSVTAYLVSNDGKLLASIPLTMMAPGLYEGSYALLPPLPQGTYLLIVNDS YGSAFSYVYFGEYNFGAILTPINDGFPAASPGQNITIIDEVLTPELTGLFTSNVTAYIYN QHGNLIDQVKLTPAPDEIQFGVYLLFFLYYANFTIPFDASPGFYNVVIQSISNTSTGLVK ADFITSFYVSPANLTLNVKVNNVVYEGELLKIFANITYPNGTPVKYGMFTATILPTSLNY EQLIIGFEAGIPLQYN*(976)STLGEWVGIYSIPSIFYGSIFQGSSVYSLAGPWNVIVSGVSWNG YNLYSTPSSFNFVNVMPYTFINNIVVSSKSLDSPLLSKINSTTYMLSNVKSNNITINGMN VILSNVIANTVTVKNSNIMITSSTINQLVLDNSSVSIIGSKIGGDN*(1185)IAVVANDSNVTIVS SVIQDSKYAFLQPNSVISLSGVNMYNVTSLSSIPAPRITYLSTTNVTTSKESIIVNITGE YLRLLGVSMNNKPVGYSVISSSPSSISLSIPFNASQLSDGQYIFTVSISDGLPYNLTFNL LNNYHLIIVQDHLKALQGSVNLLTVIAIISLIIAIIAVALLFVFTRRR
Sequence Around Glycosites (21 AA) FDARFGLPPINLTVLPVGPYH
VNPILYEIYQNTTLYHQAFHQ
GFEAGIPLQYNSTLGEWVGIY
APRITYLSTTNVTTSKESIIV
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Technique(s) used for Glycosylation DetectionConcanavalin A lectin affinity chromatography
Glycan Information
Glycan Annotation Branched sulfated heptasaccharide, Hex4(GlcNAc)2 plus sulfoquinovose where Hex is D-mannose and D-glucose.
Protein Glycosylation linked (PGL) gene(s)
Additional CommentThe glycan structure is by far the most complex one known in archaea till now.
Literature
Year of Identification2013
Year of Identification Month Wise2013.6.1
Year of Validation 2013
ReferenceGianna Palmieri, Marco Balestrieri, Jasna Peter-Katalinić, Gottfried Pohlentz, Mosè Rossi, Immacolata Fiume, and Gabriella Pocsfalvi (2013) Surface-exposed glycoproteins of hyperthermophilic Sulfolobus solfataricus P2 show a common N-glycosylation profile. J. Proteome Res., 12, 2779−2790.
Corresponding Author Gabriella Pocsfalvi
ContactInstitute of Protein Biochemistry, National Research Council of Italy, Napoli, Italy.