ProGP433 (Hypothetical protein)

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ProGP ID ProGP433 (Hypothetical protein)
Validation Status Characterized
Organism Information
Organism NameSulfolobus solfataricus P2 (DSM 1617)
Domain Archaea
Classification Phylum : Crenarchaeota
Class : Thermoprotei
Orders : sulfolobales
Family : Sulfolobaceae
Genus : Sulfolobus
Species : solfataricus
Strain : P2 (DSM 1617)
Taxonomic ID (NCBI) 273057
Genome Information
GenBank AE006641
EMBL AE006641.1
Gene Information
Gene NameSSO2712
NCBI Gene ID 27429004
GenBank Gene Sequence NZ_LT549890.1
Protein Information
Protein NameHypothetical protein
UniProtKB/SwissProt ID Q97VC2
NCBI RefSeq WP_009988641.1
EMBL-CDSAAK42823.1
UniProtKB Sequence >tr|Q97VC2|Q97VC2_SULSO Uncharacterized protein OS=Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) GN=SSO2712 PE=4 SV=1 MKALSTLAMAVIIIVVIAVVAAAAYLITSSSHHPSISTTTTPIIATNTTAPITLTVVTFS GQSANFIQYAGNLFHQLHPNVQVEVIQYPFSEYIQKELTALEAHSSQYDIIGFTSTSALD VASYLLPINESVFNFSDIIYPQEDFGGLYYNVSTNKTEVIGIAYETAVYLMAYNATIFNN QTLAQEFEQEYHMNFSPITYKNWSVVLDVDQFLTSHHITKYGFLIDDHVAHGIIDAFPAV FGWYYFRNNSLNMGNPAGLPNYNIMFEGRILPGFNYPLPSFNSSSGVQALITYRELVSYE PSPSQIQISYDNLPAFFSQGAGAFLFTSQLSYINNSKDVLLAPLPGGYAETGTDFLGISK YSSHPQLALEFLQFLVSPKVQEIAFLKYGKFPISKQAFLSLISNSSLPSYKREWLQETYY AALNATANPPNIPQTYPALIPSFNNEAFQFLTSPQYNETYAMNVLQQAANAWIKALSS
Sequence length 475 AA
Glycosylation Status
Glycosylation Type N- (Asn) linked
Experimentally Validated Glycosite(s) in Full Length ProteinN282
Glycosite(s) Annotated Protein Sequence >tr|Q97VC2|Q97VC2_SULSO Uncharacterized protein OS=Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) GN=SSO2712 PE=4 SV=1 MKALSTLAMAVIIIVVIAVVAAAAYLITSSSHHPSISTTTTPIIATNTTAPITLTVVTFS GQSANFIQYAGNLFHQLHPNVQVEVIQYPFSEYIQKELTALEAHSSQYDIIGFTSTSALD VASYLLPINESVFNFSDIIYPQEDFGGLYYNVSTNKTEVIGIAYETAVYLMAYNATIFNN QTLAQEFEQEYHMNFSPITYKNWSVVLDVDQFLTSHHITKYGFLIDDHVAHGIIDAFPAV FGWYYFRNNSLNMGNPAGLPNYNIMFEGRILPGFNYPLPSFN*(282)SSSGVQALITYRELVSYE PSPSQIQISYDNLPAFFSQGAGAFLFTSQLSYINNSKDVLLAPLPGGYAETGTDFLGISK YSSHPQLALEFLQFLVSPKVQEIAFLKYGKFPISKQAFLSLISNSSLPSYKREWLQETYY AALNATANPPNIPQTYPALIPSFNNEAFQFLTSPQYNETYAMNVLQQAANAWIKALSS
Sequence Around Glycosites (21 AA) PGFNYPLPSFNSSSGVQALIT
Technique(s) used for Glycosylation DetectionConcanavalin A lectin affinity chromatography
Glycan Information
Glycan Annotation Branched sulfated heptasaccharide, Hex4(GlcNAc)2 plus sulfoquinovose where Hex is D-mannose and D-glucose.
Protein Glycosylation linked (PGL) gene(s)
Additional CommentThe glycan structure is by far the most complex one known in archaea till now.
Literature
Year of Identification2013
Year of Identification Month Wise2013.6.1
Year of Validation 2013
ReferenceGianna Palmieri, Marco Balestrieri, Jasna Peter-Katalinić, Gottfried Pohlentz, Mosè Rossi, Immacolata Fiume, and Gabriella Pocsfalvi (2013) Surface-exposed glycoproteins of hyperthermophilic Sulfolobus solfataricus P2 show a common N-glycosylation profile. J. Proteome Res., 12, 2779−2790.
Corresponding Author Gabriella Pocsfalvi
ContactInstitute of Protein Biochemistry, National Research Council of Italy, Napoli, Italy.