ProGP438 (DnaK)

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ProGP ID ProGP438 (DnaK)
Validation Status Characterized
Organism Information
Organism NameLactobacillus plantarum
Domain Bacteria
Classification Phylum : Firmicutes
Class : Bacilli
Orders : Lactobacillales
Family : Lactobacillaceae
Genus : Lactobacillus
Species : plantarum
Taxonomic ID (NCBI) 220668
Genome Information
GenBank NC_004567.2.
EMBL AL935263
Gene Information
NCBI Gene ID 1064049
GenBank Gene Sequence NC_004567.2.
Protein Information
Protein NameDnaK
UniProtKB/SwissProt ID Q88VM0
NCBI RefSeq WP_011101636.1.
EMBL-CDSCCC79273.1
UniProtKB Sequence >sp|Q88VM0|DNAK_LACPL Chaperone protein DnaK OS=Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) GN=dnaK PE=3 SV=1 MASNKIIGIDLGTTNSAVAVLEGNEPKIITTPEGGRTVPSVVAFKDGETQVGEVAKRQAI TNPNTVASIKRHMGEAGYKVSIEGKDYTPQQISAMILQYIKGFAEDYLGDTVEKAVVTVP AYFNDAQRQATKDAGKIAGLNIERIINEPTAAALAYGLDKTDKDEKILVYDLGGGTFDVS ILELGDGVFEVLSTNGDTHLGGDDFDQKIIDWLVDGFKADNGVDLSKDKMALQRLKDAAE KAKKDLSGVSEAQISLPFISAGASGPLHLETTLTRAKFNELTADLVEKTRIPVENALKDA DLSASDLDVVILNGGSTRIPAVQEAVEKWTGKESNHSINPDEAVALGAAVQGGVITGDVK DVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSKSQVFSTAADNQPAVDIHVLQGERPM AADNKTLGRFQLTDIPAAPRGVPQIEVKFDIDKNGIVNVSAKDMGTNKEQKITIKSSSGL SDDEIDQMVKEAKENEEADKKRKEEVDLKNEVDQLIFTTDKTLKDLEGKVSEDEVKKAKD ARDALKKAQDDNNIDEMKAKKDDLNKIVQDLSVKLYQQAQEAQGAQGGADSNAAGNANSA KGSDDNTVDGDFEDLDKDKDKK
Sequence length 622 AA
Subcellular LocationIntracellular
Function Molecular chaperone
Glycosylation Status
Glycosylation Type O- (Ser) linked
Experimentally Validated Glycosite(s) in Full Length ProteinS592 and S599
Glycosite(s) Annotated Protein Sequence >sp|Q88VM0|DNAK_LACPL Chaperone protein DnaK OS=Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) GN=dnaK PE=3 SV=1 MASNKIIGIDLGTTNSAVAVLEGNEPKIITTPEGGRTVPSVVAFKDGETQVGEVAKRQAI TNPNTVASIKRHMGEAGYKVSIEGKDYTPQQISAMILQYIKGFAEDYLGDTVEKAVVTVP AYFNDAQRQATKDAGKIAGLNIERIINEPTAAALAYGLDKTDKDEKILVYDLGGGTFDVS ILELGDGVFEVLSTNGDTHLGGDDFDQKIIDWLVDGFKADNGVDLSKDKMALQRLKDAAE KAKKDLSGVSEAQISLPFISAGASGPLHLETTLTRAKFNELTADLVEKTRIPVENALKDA DLSASDLDVVILNGGSTRIPAVQEAVEKWTGKESNHSINPDEAVALGAAVQGGVITGDVK DVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSKSQVFSTAADNQPAVDIHVLQGERPM AADNKTLGRFQLTDIPAAPRGVPQIEVKFDIDKNGIVNVSAKDMGTNKEQKITIKSSSGL SDDEIDQMVKEAKENEEADKKRKEEVDLKNEVDQLIFTTDKTLKDLEGKVSEDEVKKAKD ARDALKKAQDDNNIDEMKAKKDDLNKIVQDLSVKLYQQAQEAQGAQGGADS*(592)NAAGNANS*(599)A KGSDDNTVDGDFEDLDKDKDKK
Sequence Around Glycosites (21 AA) EAQGAQGGADSNAAGNANSAK
ADSNAAGNANSAKGSDDNTVD
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Technique(s) used for Glycosylation DetectionGlycoprotein enrichment with agarose bound WGA
Technique(s) used for Glycosylated Residue(s) Detection LC-MS/MS analysis
Glycan Information
Glycan Annotation Monosaccharide (Double HexNAc, HexNAc)
Literature
Year of Identification2013
Year of Identification Month Wise2013.12.23
ReferenceFredriksen, L., Moen, A., Adzhubei, A.A., Mathiesen, G., Eijsink, V.G. and Egge-Jacobsen, W., 2013. Lactobacillus plantarum WCFS1 O-linked protein glycosylation: an extended spectrum of target proteins and modification sites detected by mass spectrometry. Glycobiology, 23(12), pp.1439-1451.
Corresponding Author Wolfgang Egge-Jacobsen
ContactNORBRAIN Mass Spectrometry Facility, Unit for Genome Dynamics, Department of Microbiology, Oslo University Hospital, Rikshospitalet, 0372 Oslo, Norway