ProGP63 (Xylanase B (Endo-1,4-beta-xylanase B))
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| ProGP ID | ProGP63 (Xylanase B (Endo-1,4-beta-xylanase B)) |
| Validation Status | Uncharacterized |
| Organism Information | |
| Organism Name | Streptomyces lividans 66 (strain 1326) |
| Domain | Bacteria |
| Classification | Phylum : Actinobacteria Class : Actinomycetia Orders : Streptomycetales Family : Streptomycetaceae Genus : Streptomyces Species : lividans Strain : 1326 |
| Taxonomic ID (NCBI) | 1916 |
| Genome Information | |
| GenBank | M64552 |
| EMBL | M64552 |
| Gene Information | |
| Gene Name | xlnB |
| Protein Information | |
| Protein Name | Xylanase B (Endo-1,4-beta-xylanase B) |
| UniProtKB/SwissProt ID | P26515 |
| EMBL-CDS | AAC06114.2 |
| UniProtKB Sequence | >sp|P26515|XYNB_STRLI Endo-1,4-beta-xylanase B OS=Streptomyces lividans GN=xlnB PE=1 SV=3 MNLLVQPRRRRRGPVTLLVRSAWAVALAALAALMLPGTAQADTVVTTNQEGTNNGYYYSF WTDSQGTVSMNMGSGGQYSTSWRNTGNFVAGKGWANGGRRTVQYSGSFNPSGNAYLALYG WTSNPLVEYYIVDNWGTYRPTGEYKGTVTSDGGTYDIYKTTRVNKPSVEGTRTFDQYWSV RQSKRTGGTITTGNHFDAWARAGMPLGNFSYYMIMATEGYQSSGSSSINVGGTGGGDSGG GDNGGGGGGCTATVSAGQKWGDRYNLDVSVSGASDWTVTMNVPSPAKVLSNWNVNASYPS AQTLTARLNGSGNNWGATIQANANWTWPSVSCSAG |
| Sequence length | 335 AA |
| Subcellular Location | Extracellular (secreted) |
| Function | It functions as an endoxylanase (EC 3.2.1.8) preferentially cleaving long-chain, water-insoluble oligoxylosides. Sequential action of xylanases B and A leads to efficient hydrolysis of xylan producing xylotriose, xylobiose and xylose. |
| Glycosylation Status | |
| Technique(s) used for Glycosylation Detection | Periodic acid-Schiff (PAS) staining |
| Protein Glycosylation linked (PGL) gene(s) | |
| Additional Comment | Xylanases from S. lividans IAF18 and IAF42 clones are glycosylated. |
| Literature | |
| Year of Identification | 1990 |
| Year of Identification Month Wise | 1990.04 |
| Reference | Kluepfel, D., Vats-Mehta, S.U.S.H.M.A., Aumont, F., Shareck, F. and Morosoli, R., 1990. Purification and characterization of a new xylanase (xylanase B) produced by Streptomyces lividans 66. Biochemical Journal, 267(1), pp.45-50. |
| Corresponding Author | D Kluepfel |
| Contact | Applied Microbiology Research Center, Armand-Frappier Institute, University of Quebec, Canada. |
