ProGP682 (Flagellin)
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| ProGP ID | ProGP682 (Flagellin) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Magnetospirillum magneticum AMB-1 |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : Alphaproteobacteria Orders : Rhodospirillales Family : Rhodospirillaceae Genus : Magnetospirillum Species : magneticum Strain : AMB-1 |
| Taxonomic ID (NCBI) | 342108 |
| Genome Information | |
| GenBank | AP007255 |
| EMBL | AP007255 |
| Gene Information | |
| Gene Name | fla (amb0684) |
| GenBank Gene Sequence | BAE49488.1 |
| Protein Information | |
| Protein Name | Flagellin |
| UniProtKB/SwissProt ID | Q2W9I7 |
| NCBI RefSeq | WP_011383127.1 |
| EMBL-CDS | BAE49488.1 |
| UniProtKB Sequence | >tr|Q2W9I7|Q2W9I7_MAGSA Flagellin OS=Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) OX=342108 GN=amb0684 PE=3 SV=1 MSDVTLTSAVRSNLLALQSTQGLVNRTQSRLSTGLKVGSAIDDPVAYFQSKALSDRASDF QGKKDNIDQGVSSLSTALQGISGVSTLVKQLKGLALNAQSASSSQIGSLVQQFNALRSQL DNLALDSQYQGQNLVAGKGQTLTVSFSNLTGSTLEVTSVDVRVGATGLNIAKAVTSNGGF QLSFDDASGAALGTEGVVKATYAGTATALTSGTYTFSYGSATVSFTVYSAGGTTGANADV EAFTTTSVLANGQDFNFRATTAGDTDELNVTQGSIQNNNHFAVEYHASTGGGVSGLAGGD SLNFVMNGLTGNSLASGNYTFSFGGYNMTFTVASAGDVTAGTFTTTNTFVNGTFGTAGGV TANTLALTLSTGTGTKENVVTFTAAVSSLGATSGPYKGQAYYSAGGISGAYGFQISAETK VGYVSGQYVLDSKLTGVVQNLVTQLDANLQVLRSHAQNLGTNVALLNTRLDFTKNYVDLL QSGSGKLTLADLNEEGANLLALQTRQQLGIQALSFAGQNEKSILSLFR |
| Sequence length | 528 AA |
| Subcellular Location | Flagellum |
| Function | Flagellin is the subunit of bacterial flagella |
| Glycosylation Status | |
| Glycosylation Type | O- (Ser/Thr) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | S103, S104, S145, S147 and T468 |
| Glycosite(s) Annotated Protein Sequence | >tr|Q2W9I7|Q2W9I7_MAGSA Flagellin OS=Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) OX=342108 GN=amb0684 PE=3 SV=1 MSDVTLTSAVRSNLLALQSTQGLVNRTQSRLSTGLKVGSAIDDPVAYFQSKALSDRASDF QGKKDNIDQGVSSLSTALQGISGVSTLVKQLKGLALNAQSASS*(103)S*(104)QIGSLVQQFNALRSQL DNLALDSQYQGQNLVAGKGQTLTVS*(145)FS*(147)NLTGSTLEVTSVDVRVGATGLNIAKAVTSNGGF QLSFDDASGAALGTEGVVKATYAGTATALTSGTYTFSYGSATVSFTVYSAGGTTGANADV EAFTTTSVLANGQDFNFRATTAGDTDELNVTQGSIQNNNHFAVEYHASTGGGVSGLAGGD SLNFVMNGLTGNSLASGNYTFSFGGYNMTFTVASAGDVTAGTFTTTNTFVNGTFGTAGGV TANTLALTLSTGTGTKENVVTFTAAVSSLGATSGPYKGQAYYSAGGISGAYGFQISAETK VGYVSGQYVLDSKLTGVVQNLVTQLDANLQVLRSHAQNLGTNVALLNT*(468)RLDFTKNYVDLL QSGSGKLTLADLNEEGANLLALQTRQQLGIQALSFAGQNEKSILSLFR |
| Sequence Around Glycosites (21 AA) | GLALNAQSASS*SQIGSLVQQF LALNAQSASSS*QIGSLVQQFN VAGKGQTLTVS*FSNLTGSTLE GKGQTLTVSFS*NLTGSTLEVT NLGTNVALLNT*RLDFTKNYVD |
| Technique(s) used for Glycosylation Detection | Periodate/biotin hydrazine method |
| Technique(s) used for Glycosylated Residue(s) Detection | Tandem mass spectrometry (LC-MS, HCD-MS/MS)) |
| Protein Glycosylation- Implication | Flagellin glycosylation is required for export and assembly of flagellum. |
| Glycan Information | |
| Glycan Annotation | A nonulosonic acid sugar (most likely pseudaminic acid) |
| Literature | |
| Year of Identification | 2018 |
| Year of Validation | 2018 |
| Reference | Sulzenbacher, G., Roig‐Zamboni, V., Lebrun, R., Guérardel, Y., Murat, D., Mansuelle, P., Yamakawa, N., Qian, X.X., Vincentelli, R., Bourne, Y. and Wu, L.F., 2018. Glycosylate and move! The glycosyltransferase Maf is involved in bacterial flagella formation. Environmental microbiology, 20(1), pp.228-240. |
| Corresponding Author | François Alberto |
| Contact | Aix Marseille Univ, CNRS, LCB UMR7283, Marseille 13402, France , International Associated Laboratory of Evolution and Development of Magnetotactic Organisms (LIA-MagMC), Centre National de la Recherche Scientifique, Marseille 13402, France |