ProGP713 (SRRP100-23)
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| ProGP ID | ProGP713 (SRRP100-23) |
| Validation Status | Uncharacterized |
| Organism Information | |
| Organism Name | Lactobacillus reuteri 100-23C |
| Domain | Bacteria |
| Classification | Phylum : Firmicutes Class : Bacilli Orders : Lactobacillales Family : Lactobacillaceae Genus : Lactobacillus Species : reuteri Strain : 100-23C |
| Taxonomic ID (NCBI) | 1598 |
| Gene Information | |
| Gene Name | srr |
| Protein Information | |
| Protein Name | SRRP100-23 |
| NCBI RefSeq | 5NY0_A |
| UniProtKB Sequence | >pdb|5NY0|A Chain A, L. reuteris SRRP binding regionEDIQADATAANASELKKALQDTSVHTIKLTDNITLTSAIELTNVSRDVTIYGNGKYINATDGNGGIFIHNTKSYTVNLTIEKATLYNQSQYGFVHMNDEGTDNITYKNITAYGGTLVWSQTHVGTKTLSLEGTVNFYSVPSYTVGGQTYSTDAFKIGTHYPNGENKDTTPAIYVSNEINIADNANIALENSATKIDIWMIADIGIHPHTTALTIGNNATLTMENGNNSALNIKLDGDTSNSFTVGEGSTVKLSAKVDNVRILPYEDSNTANVSFAKGSDVTLHAGTGSNLRMGASISNQIDFNGKATFIKDSGAYANTAYADQTRGNIEFDYYWNDQQKTGSTGVANFNPGSNVLFQAGPGASNVNTY |
| Subcellular Location | gastrointestinal tract of numerous vertebrates |
| Function | Biofilm formation and adhesion capability |
| Protein Structure | |
| PDB ID | 5NY0_A |
| Glycosylation Status | |
| Glycosylation Type | O- (Ser/Thr) linked |
| Experimentally Validated Glycosite(s ) in Mature Protein | >pdb|5NY0|A Chain A, L. reuteris SRRP binding regionEDIQADATAANASELKKALQDTSVHTIKLTDNITLTSAIELTNVSRDVTIYGNGKYINATDGNGGIFIHNTKSYTVNLTIEKATLYNQSQYGFVHMNDEGTDNITYKNITAYGGTLVWSQTHVGTKTLSLEGTVNFYSVPSYTVGGQTYSTDAFKIGTHYPNGENKDTTPAIYVSNEINIADNANIALENSATKIDIWMIADIGIHPHTTALTIGNNATLTMENGNNSALNIKLDGDTSNSFTVGEGSTVKLSAKVDNVRILPYEDSNTANVSFAKGSDVTLHAGTGSNLRMGASISNQIDFNGKATFIKDSGAYANTAYADQTRGNIEFDYYWNDQQKTGSTGVANFNPGSNVLFQAGPGASNVNTY |
| Technique(s) used for Glycosylation Detection | NMR analysis |
| Protein Glycosylation- Implication | Lactobacillus reuteri is a gut symbiont inhabiting the gastrointestinal tract of numerous vertebrates. The surface-exposed serine-rich repeat protein (SRRP) is a major adhesin in Gram-positive bacteria. Using lectin and sugar nucleotide profiling of wild-type or L. reuteri isogenic mutants provide different glycan. |
| Glycan Information | |
| Glycan Annotation | Hex-Glc-GlcNAc |
| Technique(s) used for Glycan Identification | MALDI-MS/LC-MS |
| Literature | |
| Year of Identification | 2019 |
| Reference | Latousakis, D., Nepravishta, R., Rejzek, M., Wegmann, U., Le Gall, G., Kavanaugh, D., Colquhoun, I.J., Frese, S., MacKenzie, D.A., Walter, J. and Angulo, J., 2019. Serine-rich repeat protein adhesins from Lactobacillus reuteri display strain specific glycosylation profiles. Glycobiology, 29(1), pp.45-58. |
| Corresponding Author | Nathalie Juge |
| Contact | The Gut Microbes and Health Institute Strategic Programme, Quadram Institute Bioscience, Norwich Research Park, Norwich NR4 7UA, UK |