ProGT102 (SO_2329 (EarP)/ Efp-associated protein of unknown function DUF2331)
| ProGT ID | ProGT102 (SO_2329 (EarP)/ Efp-associated protein of unknown function DUF2331) |
| Organism Information | |
| Organism Name | Shewanella oneidensis MR-1 |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : GammaProteobacteria Orders : Alteromonadales Family : Shewanellaceae Genus : Shewanella Species : oneidensis Strain : MR-1 |
| Taxonomic ID (NCBI) | 211586 |
| Genome Information | |
| Gene Bank | AE014299 |
| EMBL | AE014299 |
| Gene Information | |
| Gene Name | SO_2329 |
| NCBI Gene ID | 1170053 |
| Protein information | |
| Protein Name | SO_2329 (EarP)/ Efp-associated protein of unknown function DUF2331 |
| UniProtKB/ SwissProt ID | Q8EEP8 |
| NCBI Ref Seq | NP_717919.1 |
| UniProtKB Sequence | >tr|Q8EEP8|Q8EEP8_SHEON Uncharacterized protein OS=Shewanella oneidensis (strain MR-1) OX=211586 GN=SO_2329 PE=4 SV=1 MSTSSNASHWDIFCTVVDNYGDIGVTWRLAKQLVNEYHIPIILWVDDLNSFSHILPTLNP KLVSQCFNGVIINHWTTPLAVPYLPGKVLIEAFACELPDEVKLQLATLHKTAPQAVPLWL NLEYLSAEDWVDGCHGLPSMQVSGIKKYFYFPGFTPKTGGLICERELFAERDAWQQDPAN KLQLFESLGLKDIQAQDSVFSIFSYETDSLPALCELWQARAKNDAKIHALLPKGRSLNSL QHLLPCPVDALMPGQQIKLGDLTLHILPMTDQQGFDRLLWSCDVNIVRGEDSFLRAQWAA KPFIWHIYPQEDDYHLIKLEAFIRLYCDNLAPDIADTWSKLNFAFSQGQQSAVKTHWQNL NPVSLPLLQHAKEWPIDAINAADLATRLVQFVKKS |
| EMBL CDS | AAN55363 |
| Sequence length | 395 AA |
| Function in Native Organism | 1) EarP requires for the posttranslational modification of the conserved R32 of EF-P. |
| String | 211586.SO_2329 |
| Potential Application | 1) Inhibitors targeting EarP type glycosyltransferase helps in the development of specific antibiotics against pathogens. |
| Additional Information | 1) EarP is a rhamnosyltransferase and modifies EF-P which indirectly stimulates peptide bond formation. |
| Glycosyltransferase Information | |
| Glycosylation Type | N- (Arg) linked |
| CAZY Family | GT104 |
| EC Number (BRENDA) | 2.4.1.- |
| Mechanism of Glycan Transfer | Sequential |
| Donor Type | dTDP-l-Rhamnose |
| Donor Specificity | Nucleotide activated sugars |
| Glycan Information | |
| Glycan transferred | Monosaccharide (Rha) |
| Method of Glycan Indentification | LC-MS/MS |
| Experimental_strategies | In vivo and In vitro |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | EF-P |
| ProGPdb ID | ProGP511 |
| Litrature | |
| Year Of Validation | 2015 |
| Reference | Lassak, J., Keilhauer, E.C., Fürst, M., Wuichet, K., Gödeke, J., Starosta, A.L., Chen, J.M., Søgaard-Andersen, L., Rohr, J., Wilson, D.N. and Häussler, S., 2015. Arginine-rhamnosylation as new strategy to activate translation elongation factor P. Nature chemical biology, 11(4), pp.266-270. |
| Corresponding Author | 1 Center for Integrated Protein Science Munich, Ludwig-Maximilians-University Munich,, Munich, Germany. 2 Department of Biology I, Microbiology,Ludwig-Maximilians-University of Munich, Martinsried, Germany. |