ProGT108 (PAV_2c01640)
| ProGT ID | ProGT108 (PAV_2c01640) |
| Organism Information | |
| Organism Name | Paenibacillus alvei CCM 2051T |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Classification | Phylum : Firmicutes Class : Bacilli Orders : Bacillales Family : Paenibacillaceae Genus : Paenibacillus Species : alvei Strain : CCM 2051T |
| Taxonomic ID (NCBI) | 1206781 |
| Genome Information | |
| Gene Bank | AMBZ01000002 |
| EMBL | AMBZ01000002 |
| Gene Information | |
| Gene Name | PAV_2c01640 |
| Protein information | |
| UniProtKB/ SwissProt ID | K4ZPW8 |
| UniProtKB Sequence | >tr|K4ZPW8|K4ZPW8_PAEAL Glycosyl transferase family 2 OS=Paenibacillus alvei DSM 29 OX=1206781 GN=PAV_2c01640 PE=4 SV=1 MKSLISACMIVKDEESFIKESIASVLPYVEEVVVVDTGSKDDTVSIASYMGAKTIEMPWQ GDFSKARNAAIQHSNLPIVLMIDADERLDHRTVQALHDTARFLNENKSKAARIRIRNYTD DANTSISYATRMFPNNGKFKYMGRIHEQLCKEGNVPESIDTDIEFNHFGYMQEVIDQKQK ITRNIELLRKEASENEDNSYITYQLGKTHAIQKEYAEAEIYLRQAYQSASQDFHYCLPNI IYSLCHVLLQVKKYNDFSSIIDEAITLYPDYTDLYFMKGRAIVDNLDVTQFHLVPQLFER CVELGEVSNTQYESHVGVGTFKALYNLGIYYEMAGQKQQALDCYERCSEFGFKPAMERLL LLR |
| EMBL CDS | EJW18400.1 |
| Sequence length | 363 AA |
| Function in Native Organism | 1) Essential for flagella production and bacterial motility. |
| Additional Information | 1) Deletion studies of PAV_2c01630 and PAV_2c01640 shows that flagellin glycosylation in P. alvei is essential for the flagella formation and bacterial motility. 2) Purified Hag protein (PAV_2c01710) is modified with an O-linked trisaccharide composed of one hexose and two N-acetyl-hexosamine residues, at three sites of glycosylation. |
| Glycosyltransferase Information | |
| Glycosylation Type | O- (Ser/Thr) linked |
| EC Number (BRENDA) | 2.4.1.- |
| Mechanism of Glycan Transfer | Sequential |
| Glycan Information | |
| Method of Glycan Indentification | MALDI-TOF-MS peptide mass fingerprinting, and LC-ESI-IT-MS/MS |
| Experimental_strategies | In vivo |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | Hag |
| ProGPdb ID | ProGP520 |
| Litrature | |
| Year Of Validation | 2016 |
| Reference | Janesch, B., Schirmeister, F., Maresch, D., Altmann, F., Messner, P., Kolarich, D. and Schäffer, C., 2016. Flagellin glycosylation in Paenibacillus alvei CCM 2051T. Glycobiology, 26(1), pp.74-87. |
| Corresponding Author | Department of NanoBiotechnology, NanoGlycobiology Unit, University of Natural Resources and Applied Life Sciences, Vienna, Muthgasse 11, Vienna A-1190, Austria |