ProGT112 (SlPglB)
| ProGT ID | ProGT112 (SlPglB) |
| Organism Information | |
| Organism Name | Sulfurovum lithotrophicum |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : EpsilonProteobacteria Orders : Campylobacterales Family : Sulfurovaceae Genus : Sulfurovum Species : lithotrophicum |
| Taxonomic ID (NCBI) | 206403 |
| Genome Information | |
| Gene Bank | CP011308 |
| EMBL | CP011308 |
| Gene Information | |
| Gene Name | YH65_01055 |
| NCBI Reference Sequence | CP011308 |
| Protein information | |
| Protein Name | SlPglB |
| UniProtKB/ SwissProt ID | A0A0F6Z1U4 |
| NCBI Ref Seq | AKF24146.1 |
| UniProtKB Sequence | >tr|A0A0F6Z1U4|A0A0F6Z1U4_9PROT Peptide-binding protein OS=Sulfurovum lithotrophicum GN=YH65_01055 PE=4 SV=1 MEKTQGFERRQVLLFMLVAYLFSFAIRMIWVYQFQDNPNFYWNGQLMINTNDGYFFASGA QKVLYGMHLDNPRIPDMWSFGTVALTVLLTKVTPFSLETVILYMPAIISSLVVIPIILIA RLYKGSLWGFFAALLGSIAWSYYNRTMTGYYDTDMFSAMAPMFILYFLMKSTIDFNLKSA LYAALAIAVYPFLYDAGASIVYAMGTLYAVYMIIYHRNEDTTYNSIILVFLALVPFPLAD PYSYIVKIAVLVVLYFILLKSSFEHKKSLFVIGILFLLFMYFGNVFGLILSKISFYTATG TTGAGLHFYGVTQTIREAGKIPFETFANRISGSQIGVILSLVGYIVLVIRHRAFILALPL VGIGVFALWGGLRFTVYAVPVAAMSVIYLFHIITTTVSDKKSIYLVGMTVLTASMLYPNI KHIIQYKVPTVLNKAEVQDLDALRKVSDSKDYVMAWWDYGYPIWFYSDTNTIIDGGKHQN DNFIISKIMQTTSPEVAANLSRLAVETYVSSGYKTIADTLFKNGQKEQLDPNLLLSELEN DAYKLPKKTRNIYLYLPYRMLNIFPTVAVFGNLDLTTGKAERKIAFYPTNAVKNDNGVLQ FSNGIVFDTKKGELFFGKQKVPVKYFIVTQMTKNGETQIQTQPYHAEGQYVVVFMKSYGR FMVMDEETFRSMYVQMFILGKYDKELFEPVVSSPYSRIYKLKR |
| Sequence length | 703 AA |
| Subcellular Location | Membrane (Integral component of membrane) |
| Additional Information | 1) S. lithotrophicum OTase enzyme is able to complement C. jejuni PglB in E. coli with relaxed glycan specificities. 2) The N-linked glycan synthesized in S. lithotrophicum contains an acetylated sugar at the reducing end. |
| Glycosyltransferase Information | |
| Glycosylation Type | N- (Asn) linked |
| CAZY Family | GT66 |
| EC Number (BRENDA) | 2.4.99.18 |
| Mechanism of Glycan Transfer | En bloc |
| Acceptor specificity Sequon_1 | Asp/Glu- Asn-Xaa-Ser/Thr |
| Donor Specificity | Lipid linked sugars |
| Glycan Information | |
| Glycan transferred | Heptasaccharide, O9 O-antigen with N-acetylglucosamine (GlcNAc) and F. tularensis O-antigen with QuiNAc (2-acetamido-2,6-dideoxy-O-d-glucose). |
| Method of Glycan Indentification | MALDI-MS |
| Experimental_strategies | In vivo |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | Cj0114 |
| ProGPdb ID | ProGP219 |
| Litrature | |
| Year Of Validation | 2016 |
| Reference | Mills, D.C., Jervis, A.J., Abouelhadid, S., Yates, L.E., Cuccui, J., Linton, D. and Wren, B.W., 2016. Functional analysis of N-linking oligosaccharyl transferase enzymes encoded by deep-sea vent proteobacteria. Glycobiology, 26(4), pp.398-409. |
| Corresponding Author | Proteomics and Mass Spectrometry Core Facility, Cornell University, Ithaca, New York 14853. |