ProGT118 (EarP)
| ProGT ID | ProGT118 (EarP) |
| Organism Information | |
| Organism Name | Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : GammaProteobacteria Orders : Pseudomonadales Family : Pseudomonadaceae Genus : Pseudomonas Species : putida Strain : ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440 |
| Taxonomic ID (NCBI) | 160488 |
| Genome Information | |
| Gene Bank | AE015451 |
| EMBL | AE015451 |
| Gene Information | |
| Gene Name | earP |
| NCBI Gene ID | 1043242 |
| Protein information | |
| Protein Name | EF-P arginine 32 rhamnosyl-transferase |
| UniProtKB/ SwissProt ID | Q88LS1 |
| NCBI Ref Seq | WP_010952888.1 |
| UniProtKB Sequence | >tr|Q88LS1|Q88LS1_PSEPK EF-P arginine 32 rhamnosyl-transferase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) OX=160488 GN=earP PE=1 SV=1 MKATWDIFCSVVDNYGDIGVTWRLARQLVAEHGLAVRLWVDDLNAFTPMCPGADATAAQQ WQHGVDVRHWPAAWLPVAPADVVIGAFACQLPAAYVEAMRARPQPPLWLNLEYLSAEDWV EGCHGLPSPQPNGLRKVFFFPGFTDKTGGLLREGSLLARRDGFQQSAEARRAFLQGLGVD LVPGALLISLFAYENPQLGNWLDALATADQPCHLLVPQGRVVAGLSQWLGEGPLHVGDVR TRGALTVQVLPFVSQDDFDRLLWSCDFNAVRGEDSFVRAQWAGQPMLWHIYVQDENAHWE KLEAFLAHYRCGLSDDADAALLGLWRAWNMDFDMGQAWRAARQHWPELQQHARLWGARQA AQPDLATALVHFYRNSL |
| EMBL CDS | AAN67476 |
| Sequence length | 377 AA |
| String | 160488.PP_1857 |
| Potential Application | 1) The structures of EarP in complex with substrates should provide valuable information for the structure-guided development of its inhibitors such as EarP-containing pathogens specific antibacterials. |
| Additional Information | 1) EarP is an EF-P arginine rhamnosyltransferase which is essential for post-translational activation. 2) EF-P and the corresponding rhamnosyltransferase EarP contributes to pathogenicity in Pseudomonas putida. 3) KOW-like EF-P N-domain is sufficient for EarP-mediated rhamnosylation. |
| Glycosyltransferase Information | |
| Glycosylation Type | N- (Arg) linked |
| CAZY Family | GT104 |
| Mechanism of Glycan Transfer | Sequential |
| Donor Type | dTDP-l-Rhamnose |
| Donor Specificity | Nucleotide activated sugars |
| Glycan Information | |
| Glycan transferred | Monosaccharide (Rha) |
| Experimental_strategies | In vivo and In vitro |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | EF-P |
| ProGPdb ID | ProGP686 |
| Litrature | |
| Year Of Validation | 2017 |
| Reference | Krafczyk, R., Macošek, J., Jagtap, P.K.A., Gast, D., Wunder, S., Mitra, P., Jha, A.K., Rohr, J., Hoffmann-Röder, A., Jung, K. and Hennig, J., 2017. Structural basis for EarP-mediated arginine glycosylation of translation elongation factor EF-P. MBio, 8(5), pp.e01412-17. |
| Corresponding Author | 1 Center for integrated Protein Science Munich (CiPSM), Department of Biology I, Microbiology, Ludwig-Maximilians-Universität München, Munich, Germany.
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