ProGT143 (SvGT)
| ProGT ID | ProGT143 (SvGT) |
| Organism Information | |
| Organism Name | Streptomycetes venezulae strain 15439 |
| Clinical Implication | Non-pathogenic |
| Domain | Bacteria |
| Classification | Phylum : Actinobacteria Class : Actinomycetia Orders : Streptomycetales Family : Streptomyycetaceae Genus : Streptomyces Species : venezuelae |
| Taxonomic ID (NCBI) | 54571 |
| Genome Information | |
| Gene Bank | CP013129.1 |
| Gene Information | |
| Gene Name | SvGT |
| Protein information | |
| Protein Name | SvGT |
| NCBI Ref Seq | ALO08693.1 |
| Function in Native Organism | SvGT define the distinct donor specificity, acceptor specificity, regioselectivity, chemoselectivity, and Y(G/A/K/Q/E??G)(C/S/T?Y/N)(G/A?P/Q)G as the minimum acceptor sequon of SvGT. Although UDP-GlcNAc served as the donor in the cellular milieu, SvGT could also utilize UDP-Glc and UDP-GalNAc as donors in vitro |
| Additional Information | Also used as glycoengineering tool for modification of neo glycocin |
| Glycosyltransferase Information | |
| Glycosylation Type | S- (Cys) and O- (Ser/Thr) linked |
| CAZY Family | GT2 |
| Mechanism of Glycan Transfer | Sequential |
| Donor Type | UDP-GlcNAc, UDP-Glc, UDP-GalNAc |
| Donor Specificity | Nucleotide activated sugars |
| Glycan Information | |
| Glycan transferred | Monosaccharide (GlcNAc, Glc, GalNAc) |
| Method of Glycan Indentification | mass spectrometry and western blotting |
| Experimental_strategies | In vivo and In vitro |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | SvC |
| ProGPdb ID | ProGP1215 (SvC) |
| Litrature | |
| Year Of Validation | 2021 |
| Reference | Sharma, Y., Ahlawat, S. and Rao, A., 2021. Biochemical characterization of an inverting S/O-HexNAc-transferase and evidence of S-linked glycosylation in Actinobacteria. Glycobiology. |
| Corresponding Author | 2 CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh 160036, India |