ProGT17 (PglL)
| ProGT ID | ProGT17 (PglL) |
| Organism Information | |
| Organism Name | Neisseria meningitidis C311#3 |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : BetaProteobacteria Orders : Neisseriales Family : Neisseriaceae Genus : Neisseria Species : meningitidis Strain : MC58 |
| Taxonomic ID (NCBI) | 122586 |
| Genome Information | |
| Gene Bank | AE002098 |
| EMBL | AE002098 |
| Gene Information | |
| Gene Name | pglL (NMB0596) |
| NCBI Gene ID | 902711 |
| Protein information | |
| Protein Name | PglL |
| UniProtKB/ SwissProt ID | Q9K0K0 |
| NCBI Ref Seq | WP_002244038.1 |
| UniProtKB Sequence | >tr|Q9K0K0|Q9K0K0_NEIMB Uncharacterized protein OS=Neisseria meningitidis serogroup B (strain MC58) GN=NMB0596 PE=4 SV=1 MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALKLKPSPDFYHDAAAAAGLIVLLFL TAGKKLFDVKIPAISFLLFAMAAFWYLQARLMNLIYPGMNDIVSWIFILLAVSAWACRSL VAHFGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPLFQNIIVYSGQGVIGHIGQRN NLGHYLMWGILAAAYLNGQRKIPAALGVICLIMQTAVLGLVNSRTILTYIAAIALILPFW YFRSDKSNRRTMLGIAAAVFLTALFQFSMNTILETFTGIRYETAVERVANGGFTDLPRQI EWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQHNIYDNLLSNLFTHSHNIVLQLLAEMG ISGTLLVAATLLTGIAGLLKRPLTPASLFLICTLAVSMCHSMLEYPLWYVYFLIPFGLML FLSPAEASDGIAFKKAANLGILTASAAIFAGLLHLDWTYTRLVNAFSPATDDSAKTLNRK INELRYISANSPMLSFYADFSLVNFALPEYPETQTWAEEATLKSLKYRPHSATYRIALYL MRQGKVAEAKQWMRATQSYYPYLMPRYADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEA KPCK |
| EMBL CDS | AAF41024.1 |
| Sequence length | 604 AA |
| Subcellular Location | Membrane (Integral component of membrane) |
| Function in Native Organism | 1) It transfers the trisaccharide from the undecaprenol carrier to pilin. |
| String | 122586.NMB0596. |
| Potential Application | 1) In vivo, PglL attaches the variety of glycans to proteins this relaxed specificity can be utilized for glycoengineering novel glycan-based vaccines, therapeutics, and diagnostics tools. |
| Additional Information | 1) PglL transfers nucleotide-activated sugar (UDP-diNAcBac) on pilin in vitro. |
| Glycosyltransferase Information | |
| Glycosylation Type | O- (Ser/Thr) linked |
| EC Number (BRENDA) | 2.4.1.109 |
| Mechanism of Glycan Transfer | En bloc |
| Donor Type | UndPP-Trisaccharide |
| Donor Specificity | Lipid linked sugars |
| Accessory GT ID | ProGT17.1, ProGT17.2, ProGT17.3, ProGT17.4, ProGT17.5, ProGT17.6 |
| Glycan Information | |
| Glycan transferred | Trisaccharide (Gal(beta 1-4)Gal(alpha 1-3)2,4-diacetimido-2,4,6-trideoxyhexose). |
| Experimental_strategies | In vitro and In vivo |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | PilE |
| ProGPdb ID | ProGP99 |
| Litrature | |
| Year Of Validation | 2006 |
| Reference | Power, P.M., Seib, K.L. and Jennings, M.P., 2006. Pilin glycosylation in Neisseria meningitidis occurs by a similar pathway to wzy-dependent O-antigen biosynthesis in Escherichia coli. Biochemical and biophysical research communications, 347(4), pp.904-908. |
| Corresponding Author | Department of Microbiology, Monash University, Clayton, Australia |
| Reference | Musumeci, M.A., Hug, I., Scott, N.E., Ielmini, M.V., Foster, L.J., Wang, P.G. and Feldman, M.F., 2013. In vitro activity of Neisseria meningitidis PglL O-oligosaccharyltransferase with diverse synthetic lipid donors and a UDP-activated sugar. Journal of Biological Chemistry, 288(15), pp.10578-10587. |
| Corresponding Author | 1 Alberta Glycomics Centre, Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada,
2 Centre for High-Throughput Biology and Department of Biochemistry and Molecular Biology, University of British Columbia,
Vancouver, British Columbia V6T 14, Canada.
3 Department of Chemistry, Georgia State University, Atlanta, Georgia 30303 |