ProGT23 (AglB)

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ProGT ID ProGT23 (AglB)
Organism Information
Organism NamePyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Clinical ImplicationNon-pathogenic
DomainArchaebacteria
Classification Phylum : Euryarchaeota
Class : Thermococci
Orders : Thermococcales
Family : Thermococcaceae
Genus : Pyrococcus
Species : furiosus
Strain : ATCC 43587 / DSM 3638 / JCM 8422 / Vc1
Taxonomic ID (NCBI)186497
Genome Information
Gene BankAE009950.1
EMBLAE009950
Gene Information
Gene NamePF0156
NCBI Gene ID1467988
Protein information
Protein NameAglB 
UniProtKB/ SwissProt IDQ8U4D2
NCBI Ref SeqNC_003413.1. 
UniProtKB Sequence>tr|Q8U4D2|Q8U4D2_PYRFU Oligosaccharyl transferase stt3 subunit related protein OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) GN=PF0156 PE=1 SV=1 MVKTQIKEKKKDEKVTIPLPGKIKTVLAFLVVLAFAAYGFYIRHLTAGKYFSDPDTFYHF EIYKLVLKEGLPRYYPMADAPFGSLIGEPLGLYILPAIFYKIISIFGYNELEAFLLWPPF VGFLSVIGVYLLGRKVLNEWAGMWGAIILSVLTANFSRTFSGNARGDGPFMMLFTFSAVL MLYYLTEENKNKKIIWGTLFVLLAGISTAAWNGSPFGLMVLLGFASFQTIILFIFGKINE LREFIKEYYPAYLGILAISYLLTIPGIGKIGGFVRFAFEVFLGLVFLAIVMLYGGKYLNY SDKKHRFAVVAVIVIAGFAGAYIYVGPKLFTLMGGAYQSTQVYETVQELAKTDWGDVKVY YGVEKPNGIVFFLGLVGAMIVTARYLYKLFKDGRRPHEELFAITFYVMSIYLLWTAARFL FLASYAIALMSGVFAGYVLETVEKMKESIPIKAALGGVIAIMLLLIPLTHGPLLAQSAKS MRTTEIETSGWEDALKWLRENTPEYSTATSWWDYGYWIESSLLGQRRASADGGHARDRDH ILALFLARDGNISEVDFESWELNYFLVYLNDWAKFNAISYLGGAITRREYNGDESGRGAV TTLLPLPRYGEKYVNLYAKVIVDVSNSSVKVTVGDRECDPLMVTFTPSGKTIKGTGTCSD GNAFPYVLHLTPTIGVLAYYKVATANFIKLAFGVPASTIPGFSDKLFSNFEPVYESGNVI VYRFTPFGIYKIEENINGTWKQVYNLTPGKHELKLYISAFGRDIENATLYIYAINNEKII EKIKIAEISHMDYLNEYPIAVNVTLPNATSYRFVLVQKGPIGVLLDAPKVNGEIRSPTNI LREGESGEIELKVGVDKDYTADLYLRATFIYLVRKSGKDNEDYDAAFEPQMDVFFITKIG ENIQLKEGENTVKVRAELPEGVISSYKDELQRKYGDKLIIRGIRVEPVFIAEKEYLMLEV SASAPHH
EMBL CDSAAL80280.1
Sequence length967 AA
Subcellular LocationMembrane (Integral component of membrane)
String186497.PF0156. 
Potential Application1) Design of OST mutational variants could be used for the production of homogeneous glycoproteins in engineered mammalian and Escherichia coli cells.
Additional Information1) Pyrococcus furiosus OST contains a newly identified conserved motif DxxK (DK motif), which is located very close to the conserved WWDYG motif in the tertiary structure.
Glycosyltransferase Information
Glycosylation TypeN- (Asn) linked 
CAZY FamilyGT66
EC Number (BRENDA)2.4.99.18. 5243. 
Mechanism of Glycan TransferEn bloc
Donor TypeDolPP-Heptasaccharide
Donor SpecificityLipid linked sugars
Glycan Information
Glycan transferredHeptasaccharide (one hexouronate and two pentose residues) 
Method of Glycan IndentificationMALDI-QIT-TOF MS
Experimental_strategiesIn vitro 
Acceptor Subtrate Information
Acceptor Substrate name Polypeptide containing N-X-S/T consensus sequence
Litrature
Year Of Validation2007 
Reference Igura, M., Maita, N., Obita, T., Kamishikiryo, J., Maenaka, K. and Kohda, D., 2007. Purification, crystallization and preliminary X-ray diffraction studies of the soluble domain of the oligosaccharyltransferase STT3 subunit from the thermophilic archaeon Pyrococcus furiosus. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 63(9), pp.798-801.

Corresponding AuthorDivision of Structural Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
Reference Igura, M., Maita, N., Kamishikiryo, J., Yamada, M., Obita, T., Maenaka, K. and Kohda, D., 2008. Structure?guided identification of a new catalytic motif of oligosaccharyltransferase. The EMBO journal, 27(1), pp.234-243.

Corresponding AuthorDivision of Structural Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
Reference Igura, M. and Kohda, D., 2011. Selective control of oligosaccharide transfer efficiency for the N-glycosylation sequon by a point mutation in oligosaccharyltransferase. Journal of Biological Chemistry, 286(15), pp.13255-13260.

Corresponding AuthorDivision of Structural Biology, Medical Institute of Bioregulation, Kyushu University, Maidashi 3-1-1, Higashi-ku,Fukuoka 812-8582, Japan