ProGT43 (NGT)
| ProGT ID | ProGT43 (NGT) |
| Organism Information | |
| Organism Name | Actinobacillus pleuropneumoniae serotype 5b (strain L20) |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : GammaProteobacteria Orders : Pasteurellales Family : Pasteurellaceae Genus : Actinobacillus Species : pleuropneumoniae Strain : L20 |
| Taxonomic ID (NCBI) | 416269 |
| Genome Information | |
| Gene Bank | CP000569.1 |
| EMBL | CP000569.1 |
| Gene Information | |
| Gene Name | APL_1635 |
| NCBI Gene ID | 4849812 |
| Protein information | |
| Protein Name | NGT |
| UniProtKB/ SwissProt ID | A3N2T3 |
| NCBI Ref Seq | WP_005605627.1 |
| UniProtKB Sequence | >sp|A3N2T3|NGT_ACTP2 UDP-glucose:protein N-beta-glucosyltransferase OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) GN=APL_1635 PE=1 SV=1 MENENKPNVANFEAAVAAKDYEKACSELLLILSQLDSNFGGIHEIEFEYPAQLQDLEQEK IVYFCTRMATAITTLFSDPVLEISDLGVQRFLVYQRWLALIFASSPFVNADHILQTYNRE PNRKNSLEIHLDSSKSSLIKFCILYLPESNVNLNLDVMWNISPELCASLCFALQSPRFVG TSTAFNKRATILQWFPRHLDQLKNLNNIPSAISHDVYMHCSYDTSVNKHDVKRALNHVIR RHIESEYGWKDRDVAHIGYRNNKPVMVVLLEHFHSAHSIYRTHSTSMIAAREHFYLIGLG SPSVDQAGQEVFDEFHLVAGDNMKQKLEFIRSVCESNGAAIFYMPSIGMDMTTIFASNTR LAPIQAIALGHPATTHSDFIEYVIVEDDYVGSEECFSETLLRLPKDALPYVPSALAPEKV DYLLRENPEVVNIGIASTTMKLNPYFLEALKAIRDRAKVKVHFHFALGQSNGITHPYVER FIKSYLGDSATAHPHSPYHQYLRILHNCDMMVNPFPFGNTNGIIDMVTLGLVGVCKTGAE VHEHIDEGLFKRLGLPEWLIANTVDEYVERAVRLAENHQERLELRRYIIENNGLNTLFTG DPRPMGQVFLEKLNAFLKEN |
| EMBL CDS | ABN74719.1 |
| Sequence length | 620 AA |
| Subcellular Location | Cytoplasm |
| String | 416269.APL_1635. |
| Glycosyltransferase Information | |
| Glycosylation Type | N- (Asn) linked |
| CAZY Family | GT41 |
| EC Number (BRENDA) | 2.4.1.- |
| Mechanism of Glycan Transfer | Sequential |
| Acceptor specificity Sequon_1 | NX(S/T) |
| Donor Type | UDP-Glc and UDP-Gal ( UDP-Glc is preferred over UDP-Gal) |
| Donor Specificity | Nucleotide activated sugars (UDP and GDP linked hexose) |
| Glycan Information | |
| Glycan transferred | Monosaccharides (Glc and Gal) |
| Experimental_strategies | In vitro |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | Hexapeptide DANYTK (labeled with TAMRA at the N terminus) |
| Litrature | |
| Year Of Validation | 2011 |
| Reference | Schwarz, F., Fan, Y.Y., Schubert, M. and Aebi, M., 2011. Cytoplasmic N-glycosyltransferase of Actinobacillus pleuropneumoniae is an inverting enzyme and recognizes the NX (S/T) consensus sequence. Journal of Biological Chemistry, 286(40), pp.35267-35274. |
| Corresponding Author | Molecular Biology and Biophysics, Department of Biology, ETH Zürich, 8093 Zürich, Switzerland
Inst. of Microbiology, Dept. of Biology, ETH Zürich, Wolfgang-Pauli-Str. 10, 8093 Zürich, Switzerland |