ProGT54 (Maf1)
| ProGT ID | ProGT54 (Maf1) |
| Organism Information | |
| Organism Name | Aeromonas caviae Sch3N |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : GammaProteobacteria Orders : Aeromonadales Family : Aeromonadaceae Genus : Aeromonas Species : caviae Strain : Sch3N |
| Taxonomic ID (NCBI) | 648 |
| Genome Information | |
| Gene Information | |
| Gene Name | maf1 |
| NCBI Reference Sequence | AEZ56237.1 |
| Protein information | |
| Protein Name | Maf1 |
| UniProtKB/ SwissProt ID | H6WL43 |
| UniProtKB Sequence | >tr|H6WL43|H6WL43_AERCA Putative flagellin pseudaminyl-transferase OS=Aeromonas caviae GN=maf1 PE=4 SV=1 MSLNIDVALSKAEHGAQRLQQQAEQEAAMAIALPLRFEQNMGAFRQYIPNIADIYEAYQP TRPFKIFCTENGQPNLMWVDESVAVYGAEPYLICETLAKEFIEKGALSKLSFSQETNPLG FMHVEYLNKLNIYLDEICENETLLGYVPSEVPSALVFGVGLGYHLGYLYERCKIGTLFLF EPDLDLFYASLYCFDWAPLLDYLHQENLGVHILLGQDEQTIVKDFSSAIHRRGSFLAANS FIMWGYQNDKIRALIEKVQQEYYLLVMGWGFFDDNLIALSHSITNIERSVPFLRKGEKIS SKYQRAPVFVIGNGPSLDEALDVIKEHKDNAILVACGSAISALHKIGIKPDIYVATERTK IVYDFLLNLNDPEYLHDIIFLSTDVIHPDCSTLFKQSALIFKAGEPGSSVYQTNFPTLPH YAVIGGVNPLVGNIGVSAPIYLGFTNLYLFGLDNGYKNKGHHHSRYSSYYNNEDNAKALG NLMYSDKQWSREGNFGGEIISNIMFDTSRRVVEQVLSTNENVQCFNCSDGVKIEGAKALP ASEVKLFHPINKHIILDEITRLFSPIPLSKQNFEPFIDVDYFNYFVDKMMGEWQEVFSSR NQINQLMLRNFNYLSQISSTNQRHISQVMIGSMNYVFTLLSTILYSIEDEDKALHFMKPA IDLWLEFLAEAKKMYPRALDSVDMIDNEVIVLFKK |
| EMBL CDS | AEZ56237.1 |
| Sequence length | 695 AA |
| Function in Native Organism | 1) Aeromonas caviae Sch3N maf1 is a putative flagellin pseudaminyl transferase and essential in glycosylation of flagellar protein which helps in swarming motility of bacteria. |
| Additional Information | 1) Overexpression of Maf1 inhibits motility, indicating its a dominant negative effect, possibly caused by high amounts of glycosylated flagellin inhibit assembly of the flagellum. |
| Glycosyltransferase Information | |
| Glycosylation Type | O- (Ser/Thr) linked |
| EC Number (BRENDA) | 2.4.1.- |
| Donor Type | CMP-Pse5Ac7Ac |
| Accessory GT ID | ProGT54.1, ProGT54.2, ProGT54.3, ProGT54.4, ProGT54.5 |
| Glycan Information | |
| Glycan transferred | Pse5Ac7Ac |
| Method of Glycan Indentification | LC-MS/MS |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | FlaA |
| ProGPdb ID | ProGP296 |
| Acceptor Substrate name | FlaB |
| ProGPdb ID | ProGP297 |
| Litrature | |
| Year Of Validation | 2012 |
| Reference | Parker, J.L., Day?Williams, M.J., Tomas, J.M., Stafford, G.P. and Shaw, J.G., 2012. Identification of a putative glycosyltransferase responsible for the transfer of pseudaminic acid onto the polar flagellin of Aeromonas caviae Sch3N. Microbiologyopen, 1(2), pp.149-160. |
| Corresponding Author | Academic Unit of Immunology and Infectious Diseases, Department of Infection and Immunity, University of Sheffield Medical School, Sheffield, S10 2RX United Kingdom. |
| Reference | Parker, J.L., Lowry, R.C., Couto, N.A., Wright, P.C., Stafford, G.P. and Shaw, J.G., 2014. Maf?dependent bacterial flagellin glycosylation occurs before chaperone binding and flagellar T3SS export. Molecular microbiology, 92(2), pp.258-272. |
| Corresponding Author | Department of Infection and Immunity, University of Sheffield, Sheffield, S10?2RX, UK. |