ProGT72 (Tot/AglB)

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ProGT ID ProGT72 (Tot/AglB)
Organism Information
Organism NameRalstonia solanacearum GMI1000
Clinical ImplicationPlant pathogen
DomainBacteria
Classification Phylum : Proteobacteria
Class : BetaProteobacteria
Orders : Burkholderiales
Family : Burkholderiaceae
Genus : Rolstonia
Species : solanacearum
Strain : GMI1000
Taxonomic ID (NCBI)267608
Genome Information
Gene BankAY596297.1
EMBLAY596297
Gene Information
Gene Nametot
NCBI Gene ID3130386
NCBI Reference SequenceAY596297
Protein information
Protein NameTot/AglB 
UniProtKB/ SwissProt IDQ5V4T7
NCBI Ref SeqWP_011223023.1
UniProtKB Sequence>tr|Q5V4T7|Q5V4T7_HALMA Oligosaccharyl transferase OS=Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) OX=272569 GN=tot PE=3 SV=1 MSQSRGYLDDNPELESALEWAERWYHVPVLLVLLGFMLWNRVRSWENFIVDGEVLFSGND AWYHYRSTQYVVNNWPATMPFDPWTYFPYGTSTGQFGTLLDQIVATVALIIGLGSPSEQT VAMTLLFAPAVLGTLVAVPTYLVGRRLGGRLGGVTAVTVMAFSTGGLLQRSRVGFSDHHV AEALFQILGVLGVMVAVSVAARDKPIYEQFIERDIDALRDTISWSMLAGVAIAMYLWVWP PGVLLLGILGVFFLLRLCLEYVHDSSPEHTAIAGAITMVTAAVVSASRINVLEITATAQS LLQPGLALAVGFGCVFMAWLARFMEQREYGQYVYPATVFGILAFGAVLMAVLTPDLWSFF TNNVMRIIGLTTSETANTVGEASPLRNAGVLFDRHGFAVIVAGIGGLLLLGKQFLSDDAP AEEMLVVVWAVFILLATFTQRRFAYYLVAPIAVLSGMVVGRFIRWFDFSADDGIEYYQVM TILTVLLVIVVPLFAFGTTPVEAGSTGPGPSIQGWDESLQWMEGNTPAEGAYGSGGDATL EHYGTYERQEDFDYQEGQYGVLSWWDYGHWITTRAERVPNANPFQQGTETVAPFLVAQNE TQANNILDSTDEDDAKTRYIAVDWKMAETNGNLNGKFFAPATFADGVEPGDYYSRMYIQS QDGARQITFQQQAYYETMTARLYRFHGSAAEPQPIVVDWENKQTGQGAQYRGAPTNEQEP EGQRQGQIVRQFESMEQARQYVEEDATAQIGGFGDNPSERVSAMEHYRLVGSSETEAFPN NPRYQSSAWAKIFERVPGATIEGTGPANATVRAAVQMRNPATNKTFVYRQRTQTDQNGNF EMTVPYSTTGYDDWGTDEGYTNVSVRAETQYQFTAIGANNGNRTGFTGATEVTEGQVIGE DDDAATVELEPVTIQQGNGTSGESRTPMTEDAAEAGA
EMBL CDSAAV45465.1
Sequence length937 AA
Subcellular LocationMembrane (Integral component of membrane)
String272569.rrnAC0431
Additional Information1) AglB of Har. marismortui is able to complement Hfx. volcanii AglB in AglB deleted Hfx. volcanii cell.
Glycosyltransferase Information
Glycosylation TypeN- (Asn) linked 
CAZY FamilyGT66
EC Number (BRENDA)2.4.1.-
Mechanism of Glycan TransferEn bloc
Acceptor specificity Sequon_1Asn-Xaa-Ser/Thr
Donor SpecificityLipid linked sugars
Glycan Information
Glycan transferredPentasaccharide comprising a Monosaccharide ( hexose), two hexuronic acids, a methyl ester of hexuronic acid, and a Monosaccharide (Man) 
Method of Glycan IndentificationLC-ESI MS/MS
Acceptor Subtrate Information
Acceptor Substrate name S-layer glycoprotein
ProGPdb ID ProGP71
Litrature
Year Of Validation2014 
Reference Cohen-Rosenzweig, C., Guan, Z., Shaanan, B. and Eichler, J., 2014. Substrate promiscuity: AglB, the archaeal oligosaccharyltransferase, can process a variety of lipid-linked glycans. Applied and environmental microbiology, 80(2), pp.486-496.

Corresponding AuthorDepartment of Life Sciences, Ben Gurion University of the Negev, Beersheva, Israel.