ProGT78 (GtfB)
| ProGT ID | ProGT78 (GtfB) |
| Organism Information | |
| Organism Name | Staphylococcus aureus |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Classification | Phylum : Firmicutes Class : Bacilli Orders : Bacillales Family : Staphylococcaceae Genus : Staphylococcus Species : aureus Subspecies : aureus Strain : USA300_TCH959 |
| Taxonomic ID (NCBI) | 450394 |
| Genome Information | |
| Gene Bank | AASB02000025 |
| EMBL | AASB02000025 |
| Gene Information | |
| Gene Name | gtfB |
| NCBI Reference Sequence | AASB02000025 |
| Protein information | |
| Protein Name | GtfB |
| UniProtKB/ SwissProt ID | A0A0E1VMC9 |
| NCBI Ref Seq | WP_000593162.1 |
| UniProtKB Sequence | >tr|A0A0E1VMC9|A0A0E1VMC9_STAA3 Glycosyltransferase stabilizing protein Gtf2 OS=Staphylococcus aureus subsp. aureus USA300_TCH959 GN=gtfB PE=3 SV=1 MIKLFDYFNDHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVISPYQFFADNHNTENMKP RFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPRIVSRVEWFDDQQRVRFVD YYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVTSDVILDWQGKSYFFPSKLAF VLFFIKQLEITEHHFVINSLALPFSVLYNLPSNGSDVLVWQEQCDGNVPGNMQLMCKGDM KRHCNIIIPDKNEYETMLNIADAKVQSRILQGGYLYNYRSRNRYTKEIVILTNSDQLRNI KVLVETLPDFNFHIAAITEMSDKLMQLDQYANVHLYPSINIDRVNELYQLCDIYLDINEG NEILNAVEQAFDYELLILGYRQTAHHAKVTLSEHLFEHNDEITMESKDQLIQMLESLKDQ QQFRDALLAQKAHAHEISREQFEQVFKQALES |
| EMBL CDS | EES94653.1 |
| Sequence length | 452 AA |
| Subcellular Location | Membrane (Integral component of membrane) |
| Additional Information | 1) The deletion studies of GtfA and GtfB show that both GtfA-GtfB is involved in the transfer of GlcNAc residues to the peptide backbone of SraP. |
| Glycosyltransferase Information | |
| Glycosylation Type | O- (Ser/Thr) linked |
| CAZY Family | GT8 |
| EC Number (BRENDA) | 2.4.1.- |
| Mechanism of Glycan Transfer | Sequential |
| Donor Type | UDP-GlcNAc |
| Donor Specificity | Nucleotide activated sugars |
| Glycan Information | |
| Glycan transferred | Oligosaccharides |
| Method of Glycan Indentification | sWGA blotting |
| Experimental_strategies | In vivo |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | SraP |
| ProGPdb ID | ProGP245 |
| Litrature | |
| Year Of Validation | 2014 |
| Reference | Li, Y., Huang, X., Li, J., Zeng, J., Zhu, F., Fan, W. and Hu, L., 2014. Both GtfA and GtfB are required for SraP glycosylation in Staphylococcus aureus. Current microbiology, 69(2), pp.121-126. |
| Corresponding Author | Department of Laboratory Medicine, Union Hospital, Tongji Medical College Huazhong, University of Science and Technology, Wuhan, China. |