ProGT80 (AglB)
| ProGT ID | ProGT80 (AglB) |
| Organism Information | |
| Organism Name | Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium) |
| Clinical Implication | Non-pathogenic |
| Domain | Archaebacteria |
| Classification | Phylum : Euryarchaeota Class : Halobaccteria Orders : Halobacteriales Family : halobacteriaceae Genus : Halobacteria Species : salinarum Strain : ATCC 700922 / JCM 11081 / NRC-1 |
| Taxonomic ID (NCBI) | 64091 |
| Genome Information | |
| Gene Bank | AM774415 |
| EMBL | AM774415 |
| Gene Information | |
| Gene Name | aglB |
| NCBI Reference Sequence | AM774415 |
| Protein information | |
| Protein Name | AglB |
| UniProtKB/ SwissProt ID | B0R4T2 |
| NCBI Ref Seq | WP_010902766.1 |
| UniProtKB Sequence | >tr|B0R4T2|B0R4T2_HALS3 Dolichyl-monophosphooligosaccharide--protein glycotransferase AglB OS=Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1) GN=aglB PE=4 SV=1 MSETTDASGLSAGLPSRALGALKDWYHVPVLGAVLAFMFWVRVQSWDNFLQNGEVYLSGN DAYYHLRQVTYTVHHFPETMPFDPWTNFPHGTLAGQFGTLFDQILAAVALVVGMGSPSEH TIAMTLLLAPPVFGALLVVPTYVMGRRIAGRLGGLFGAVILGLLPGYFLQRTLAGAADHN GAEPLFMTLAVVGLMVALSVAEREKPVFEQFRAWDATGLRDVVGWSVLAGVATAVYMWVW PPGVLLVGIFGAFFLVKLVGDYVTGTSPDHVAVVGAVSMTTTALLMFAPLGESGFGVSGF SFLQPVFALGVAVGCVVLAWLARVFDERSLSPAAYVATVAGTLVVVVGALALVAPGFWSS LQSNLLNYIGLSSTADTRTIGEAQPFLFRTGRYGLGMFGVVFLEYGTAFFSALVGAGAIL LKPHLTSGSVRRIVGVAGATAVLAIVAVSPAVPAAIGGVVGLGGQLTALLIAAVVLAAIA ATGQYDADKLLLVVWGAFVTSAAFTQVRFNYYLVVPVVVLNAYVLRAVLAAVDLDRPVAA IEGVSWYQVGTVVIVALIVLVPVAAPAVAEATNNDSASGPVSPIKLSNNQNQPVPLQSAI TAGQGQGPGGVVAWDDAMDWYQSHTPKQGTYGGADNADALDYNGTYSQTEDFDYPAGSFG VLSWWDYGHWMTVQGHAIPHANPFQQGATSAANFLLADDEQQSESVLADIEEDDAKNRYV AVDWKMVMPPLPYLSSGQSKFSAPVVFYDGPRDLSQGDFYQQAYNADWEENRISNARFFR QSPLLKKQAYYESMMVRLYRYHGSAKQAEPVVADWRRSVNTGQGTAAAFDAATNNTKQFD SMADAKAYVRNDSTAQIGGTGSLPEADVAALEHYRLVGVSANHTAPANLWYLGGELSTEY YPTSVKLFERVDGAAVTGTGPANTTVKASVELNLTQMESNEGTHPSFTYTQYADTDADGE FTMRLPYSTTGYDELGPANGHTNVSVRATGPYTFTAGDQAGNASAVAADTASVSESQVVS DEATPVTVSLDPTGGAAGNETATNGSVATPTADGESADGDGTDADTTNSTAGALPARVGA ELTHRGH |
| EMBL CDS | CAP13747.1 |
| Sequence length | 1087 AA |
| Subcellular Location | Membrane (Integral component of membrane) |
| Potential Application | 1) Understanding of N-glycosylation in Archaea could help to develop glycoengineering tool. |
| Additional Information | 1) Deleted Hfx. volcanii AglB can be functionally replaced by Hbt. salinarum AglB. |
| Glycosyltransferase Information | |
| Glycosylation Type | N- (Asn) linked |
| CAZY Family | GT66 |
| EC Number (BRENDA) | 2.4.1.- |
| Mechanism of Glycan Transfer | En Bloc |
| Acceptor specificity Sequon_1 | Asn-Xaa-Ser |
| Donor Type | Dol-PP-Pentasaccharide |
| Donor Specificity | Lipid linked sugars |
| Accessory GT ID | ProGT80.1, ProGT80.2, ProGT80.3, ProGT80.4, ProGT80.5, ProGT80.6, ProGT80.7, ProGT80.8, ProGT80.9, ProGT80.10, ProGT80.11, ProGT80.12, ProGT80.13 |
| Glycan Information | |
| Glycan transferred | Pentasaccharide (Hex-(HexA)2-HexA(methyl ester)-Hex ) |
| Method of Glycan Indentification | LC-ESI-MS |
| Experimental_strategies | In vivo |
| Acceptor Subtrate Information | |
| Litrature | |
| Year Of Validation | 2014 |
| Reference | Cohen-Rosenzweig, C., Guan, Z., Shaanan, B. and Eichler, J., 2014. Substrate promiscuity: AglB, the archaeal oligosaccharyltransferase, can process a variety of lipid-linked glycans. Applied and environmental microbiology, 80(2), pp.486-496. |
| Corresponding Author | Department of Life Sciences, Ben Gurion University of the Negev, Beersheva, Israel. |