ProGT98 (PglB)
| ProGT ID | ProGT98 (PglB) |
| Organism Information | |
| Organism Name | Nitratiruptor sp. SB155-2 |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : EpsilonProteobacteria Orders : Nautiliales Family : Nitratiruptoraceae Genus : Nitratirruptor Strain : SB155-2 |
| Taxonomic ID (NCBI) | 387092 |
| Genome Information | |
| Gene Bank | AP009178 |
| EMBL | AP009178 |
| Gene Information | |
| Gene Name | NIS_1250 |
| Protein information | |
| Protein Name | PglB |
| UniProtKB/ SwissProt ID | A6Q4E9 |
| NCBI Ref Seq | WP_012082621.1? |
| UniProtKB Sequence | >tr|A6Q4E9|A6Q4E9_NITSB Oligosaccharide transferase OS=Nitratiruptor sp. (strain SB155-2) GN=NIS_1250 PE=4 SV=1 MYVQKKSMVMILAIVTAYIFSYLFHLYWIGWASHNPEFFWNGQLMINNVDGYFFGSGAQK ILYHEHLDNPRLLDVWRYGTAVITAYIVKLLPISLDTAMLYLPPVLSSLVVIPIILVGRL YGNVIWGFLSALIASIGWSYYNRTLAGYYDTDMFSAMLPMFVFFFLISAIKSRSLNYILA ASLTVILYPWLYDQGLSIVYAMGIMMFLYLVLFYRYEKFTYKAIIIFSFALMPILWMFKL LLVIVLWFLLKTYDTELKKFQIAAGVSFLAFILIGNVFGIILHKVFSYTSHTDQIAGLHF LNVNETVREAGKIPFEVVADRIVGSLLGLVIAVAGYILLLIRNKEFIIALPLWGIGFFAY FGGLRFTVYAVPIAAMSAVYFFFWLSERFEAKKVRIAAVSLGTLFLLAPNITHITGCCEK VSWLDSIKEFYPLKSYPYLTPTTLLKSEVALLNEFNKKSFPKDYAITWWDYGYPIWYYAD VNTLIDGGKHNEDNFLVSKILTTSDQRLAANLAKLSIKVYVDTNKTVAPQLFIKNKKVIN VDEFFANLSTKEYKPPKLNRDIYLILPHRMFDIFPTVAYFSERNLNTGKVYSRKFYYKNR VVQKGGKLYIGQLIVDLRRAVILIGNQQAPIKNVYIIGIDQNGKSHYKEQKIRDKGLSLI INKSFGEAIIADNKYINSIFFQMYLFDNYDPMLFEPVILSPWMKIYRIK |
| EMBL CDS | BAF70358.1 |
| Sequence length | 709 AA |
| Subcellular Location | Membrane (Integral component of membrane) |
| String | 387092.NIS_1250 |
| Additional Information | 1) It is able to transfer glycans to the acceptor sites, predominantly to the sites having a negatively charged residue in the ?2 position relative to the asparagine. |
| Glycosyltransferase Information | |
| Glycosylation Type | N- (Asn) linked |
| CAZY Family | GT66 |
| EC Number (BRENDA) | 2.4.99.18 |
| Mechanism of Glycan Transfer | En bloc |
| Acceptor specificity Sequon_1 | Asn-Xaa-Ser |
| Donor Type | UndPP-Heptasaccharide |
| Donor Specificity | Lipid linked sugars |
| Glycan Information | |
| Glycan transferred | Heptasaccharide |
| Method of Glycan Indentification | LC-MS |
| Experimental_strategies | In vivo and In vitro |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | scFv13-R4 DQNAT |
| Acceptor Substrate name | TAMRA-GDQNATAF |
| Litrature | |
| Year Of Validation | 2015 |
| Reference | Ollis, A.A., Chai, Y., Natarajan, A., Perregaux, E., Jaroentomeechai, T., Guarino, C., Smith, J., Zhang, S. and DeLisa, M.P., 2015. Substitute sweeteners: diverse bacterial oligosaccharyltransferases with unique N-glycosylation site preferences. Scientific reports, 5(1), pp.1-13. |
| Corresponding Author | Proteomics and Mass Spectrometry Core Facility, Cornell University, Ithaca, New York 14853. |