ProGP111 (S-layer glycoprotein)
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| ProGP ID | ProGP111 (S-layer glycoprotein) |
| Validation Status | Uncharacterized |
| Organism Information | |
| Organism Name | Thermoanaerobacterium (Clostridium) thermosaccharolyticum E207-71 |
| Domain | Bacteria |
| Classification | Phylum : Firmicutes Class : Clostridia Orders : Thermoanaerobacterales Family : Thermoanaerobacteraceae Genus : Thermoanaerbacter Species : pseudehanolicus Strain : E207-71 |
| Taxonomic ID (NCBI) | 340099 |
| Genome Information | |
| GenBank | CP000924 |
| EMBL | CP000924 |
| Protein Information | |
| Protein Name | S-layer glycoprotein |
| Subcellular Location | Surface |
| Function | Structural component of the surface layer. |
| Glycosylation Status | |
| Glycosylation Type | O- (Tyr) linked |
| Technique(s) used for Glycosylation Detection | Chemical deglycosylation with TFMS |
| Glycan Information | |
| Glycan Annotation | Linkages: β-Glc-O-Tyr, β-Gal-O-Tyr. Glucosyl tyrosine is found in short-chain glycans while galactosyl tyrosine in long-chain glycans. It is the first report where the unusual monosaccharide 3-acetamido-3,6-dideoxy-D-glucose (quinovosamine) has been found to be present in S-layer glycoproteins. Repeating hexasaccharide [→4)[β-D-Quip3NAc-(1→6)-β-D-Galf-(1→4)-α-L-Rhap-(1→3)]-α-D-Manp-(1→4)-β-D-Galp-(→4)-β-D-Glcp-(1→]n=17 is present. Microheterogeneity is also observed. |
| BCSDB ID | 23144 |
| GlyTouCan | G67777CO |
| Protein Glycosylation linked (PGL) gene(s) | |
| Additional Comment | 100-210 kDa ladder-like bands represented glycosylated S-layer subunits. |
| Literature | |
| Year of Identification | 1995 |
| Year of Identification Month Wise | 1995.03 |
| Reference | Messner, P., Steiner, K., Zarschler, K. and Schäffer, C., 2008. S-layer nanoglycobiology of bacteria. Carbohydrate Research, 343(12), pp.1934-1951. |
| Corresponding Author | Paul Messner |
| Contact | University of Natural Resources and Life Sciences Vienna, Center for NanoBiotechnology A-1180 Vienna, Gregor-Mendel-Strasse 33, Austria. |
| Reference | Altman, E., Schäffer, C., Brisson, J.R. and Messner, P., 1996. Isolation and characterization of an amino sugar-rich glycopeptide from the surface layer glycoprotein of Thermoanaerobacterium thermosaccharolyticum E207-71. Carbohydrate research, 295, pp.245-253. |
| Corresponding Author | Eleonora Altman |
| Contact | Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ont., Canada |
| Reference | Altman, E., Schäffer, C., Brisson, J.R. and Messner, P., 1995. Characterization of the glycan structure of a major glycopeptide from the surface layer glycoprotein of Clostridium thermosaccharolyticum E207‐71. European Journal of Biochemistry, 229(1), pp.308-315. |
| Corresponding Author | Eleonora Altman |
| Contact | Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ont., Canada |