ProGP1281 (DNA-directed RNA polymerase subunit A’)

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ProGP ID ProGP1281 (DNA-directed RNA polymerase subunit A’)
Validation Status Uncharacterized
Organism Information
Organism NameHaloferax volcanii DS2
Domain Archaea
Classification Phylum : Euryarchaeota
Class : Halobacteria
Orders : Haloferacales
Family : Haloferacaceae
Genus : Haloferax
Species : volcanii
Strain : DS2
Taxonomic ID (NCBI) 2246
Genome Information
GenBank M62816
EMBL M62816
Organism Additional Information It is an easily culturable moderate halophile
Gene Information
Gene NameHVO_0349
Protein Information
Protein NameDNA-directed RNA polymerase subunit A’
UniProtKB/SwissProt ID D4GZX6
UniProtKB Sequence >tr|D4GZX6|D4GZX6_HALVD DNA-directed RNA polymerase subunit OS=Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) OX=309800 GN=rpoA1 PE=3 SV=1 MSMQTPKEIGSIQFGLMDPETYRDMSATKVITADTYDDDGYPIDMGLMDPRLGVIDPGLQCRTCGQHSGSCNGHFGHIELAAPVIHVGFTKLIRRLLRSTCRECGRLSLTDEEADEYRDKLGRTKELGDDWSDVMKSAVRQARKANRCPHCGSPQHDIKHEKPTTYYEVQNVLAGDYSERIAEAMQPDPDDEDDEGVDPVTLANETGLDAERVNQVLAGEFRPVGDDRKALEKALDLDLTEEDMNKLMPSDIRDWFEDVPDEDLVTLGIDPDRSRPEWMILTVLPVPPVTARPSITLDNGQRSEDDLTHKLVDIIRINQRFMENREAGAPQLIIEDLWELLQYHVTTFIDNEISGTPPARHRSGRPLKTLSQRLKGKEGRFRGSLSGKRVNFSARTVISPDPTLSLNEVGVPDRVASEMTQTMNVTERNVDEARQYVRNGPESHPGANYVRRPDGRRLKVTEKNCEELAEKVELGWEVNRHLVDGDIVIFNRQPSLHRMSIMAHEVVVMPYKTFRLNTVVCPPYNADFDGDEMNMHALQNEEARAEARVLMRVQEQILSPRFGENIIGAIQDHISGTYLLTHDNPEFVETQALDLLRATRVDTLPEPAGENEGGQPYWTGQQIFSELLPDDLNMEFTSKVGDTVSIEDGQLVQGTIDEDAVGAFGGEIVDALAKDYSKTRSRIFINEIASLAMRAIMHFGLSIGIDDESIPDEATAQVDEAIDAAYDKIQELIEIYEAGELESLPGRSVDETLEMKIMQRLGKARDSAGEIAEDHFEEDNPAVVMSKSGARASMLNLTQMAGCVGQQAVRGERINRGYEGRTLSHYQKGDLSAEAHGFVENSYRAGLTPREFFFHAMGGREGLVDTAVRTSKSGYLQRRLINALSELEAQYDGSVRDTSGTIVQFEFGEDGTSPVKVSSDDETPIDVENIADRILTSEFSSDEEKERFLGERAPPTNLSEHAEPRVDARAGVESDD
Subcellular Locationcytosolic
Glycosylation Status
Glycosylation Type O- (Ser/Thr) linked
Technique(s) used for Glycosylation DetectionUPLC coupled via a nanospray source to a Q-Exactive HFX tandem MS
Glycan Information
Technique(s) used for Glycan Identification UPLC coupled via a nanospray source to a Q-Exactive HFX tandem MS
Protein Glycosylation linked (PGL) gene(s)
Additional CommentGlycosylation is predicted by glycoproteomic in-silico analysis of raw experimental data available on PRIDE and jPOST.
Literature
Year of Identification2021
ReferenceSchulze, S., Pfeiffer, F., Garcia, B.A. and Pohlschroder, M., 2021. Comprehensive glycoproteomics shines new light on the complexity and extent of glycosylation in archaea. PLoS biology, 19(6), p.e3001277.
Corresponding Author Mechthild Pohlschroder
ContactDepartment of Biology, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America