| Technique(s) used for Glycosylation Detection | Deduced Crystal structure |
| Technique(s) used for Glycosylated Residue(s) Detection | Crystallographic analysis (electron density maps) |
| Protein Glycosylation- Implication | No specifc role has been proposed for O-linked oligosaccharides in chondrotinase AC but their positioning and interaction in the structure of chondroitinase AC in the case of Ser455 attached oligosaccharide, presents a possibility for some role in protein folding, as in mammalian systems. |
| Glycan Information |
| Glycan Annotation | Linkages: Man-Ser.
The approx. weights of glycans attached to S328 and S455 were 1190Da and 1080 Da, respectively.
Branched heptasaccharide is: galactose-β(1–4)[galactose-α(1–3)](2-O-Me)fucose-β(1–4)xylose-β (1–4)glucuronic acid-α(1–2)[rhamnose-α(1–4)]mannose-α(1-O)Ser.
Only tetrasaccharide glycan observed in crystal structure: Man-(Rha)-GlcUA-Xyl (xylose-β (1–4)glucuronic acid-α(1–2)[rhamnose-α(1–4)]mannose-α(1-O)Ser. |
| GlyTouCan | G07644FE |
| Technique(s) used for Glycan Identification | Crystallographic analysis (electron density maps) |
| Literature |
| Year of Identification | 1996 |
| Year of Identification Month Wise | 1996.11 |
| Year of Validation | 1996 |
| Reference | Huang, W., Boju, L., Tkalec, L., Su, H., Yang, H.O., Gunay, N.S., Linhardt, R.J., Kim, Y.S., Matte, A. and Cygler, M., 2001. Active site of chondroitin AC lyase revealed by the structure of enzyme− oligosaccharide complexes and mutagenesis. Biochemistry, 40(8), pp.2359-2372. |
| Corresponding Author | Miroslaw Cygler |
| Contact | Biotechnology Research Institute, 6100 Royalmount Avenue, Montréal, Québec H4P 2R2 Canada. |
| Reference | Féthière, J., Eggimann, B. and Cygler, M., 1999. Crystal structure of chondroitin AC lyase, a representative of a family of glycosaminoglycan degrading enzymes. Journal of molecular biology, 288(4), pp.635-647. |
| Corresponding Author | Miroslaw Cygler |
| Contact | Biotechnology Research Institute, 6100 Royalmount Avenue, Montréal, Québec H4P 2R2 Canada. |
| Reference | Laliberte, M., Eggimann, B., Zimmermann, J.J.F., Huang, L. and van Halbeek, H., 1996. Determination of the glycosylation sites of glycosaminoglycan lyases from Flavobacterium heparinum. Protein Sci, 5(Suppl 1), p.435s. |
| Reference | Huang, W., Boju, L., Tkalec, L., Su, H., Yang, H.O., Gunay, N.S., Linhardt, R.J., Kim, Y.S., Matte, A. and Cygler, M. (2001) Active site of chondroitin AC lyase revealed by the structure of enzyme-oligosaccharide complexes and mutagenesis. Biochemistry, 40, 2359-2372. [PubMed: 11327856] |
| Author | Huang, W., Boju, L., Tkalec, L., Su, H., Yang, H.O., Gunay, N.S., Linhardt, R.J., Kim, Y.S., Matte, A. and Cygler, M. |
| Research Group | Biotechnology Research Institute, 6100 Royalmount Avenue, Montréal, Québec H4P 2R2 Canada. |
| Corresponding Author | Cygler, M. |
| Contact | Biotechnology Research Institute, 6100 Royalmount Avenue, Montréal, Québec H4P 2R2 Canada. |
| Reference | Fethiere, J., Eggimann, B. and Cygler, M. (1999) Crystal structure of chondroitin AC lyase, a representative of a family of glycosaminoglycan degrading enzymes. J Mol Biol, 288, 635-647. [PubMed: 10329169] |
| Author | Féthière J ,Eggimann B ,Cygler M |
| Research Group | 1) Biotechnology Research Institute, NRC, 6100 Royalmount Avenue, Montréal Québec, H4P 2R2, Canada
2) Ibex Technologies, 5485 Paré Street, Montréal, Québec H4P 2R2, Canada
3) Montréal Joint Centre for Structural Biology, Montreal Québec, Canada |
| Corresponding Author | Cygler M |
| Contact | 1) Biotechnology Research Institute, NRC, 6100 Royalmount Avenue, Montréal Québec, H4P 2R2, Canada
2) Montréal Joint Centre for Structural Biology, Montreal Québec, Canada |
| Reference | M. Laliberté, B. Eggimann, J. Zimmerman, L. Huang and H. van Halbeek. (1996) Determination of the glycosylation sites of glycosaminoglycan lyases from Flavobacterium heparinum. Protein Sci.1996, 5 (suppl. 1) 435-s. |
| Author | M. Laliberté, B. Eggimann, J. Zimmerman, L. Huang H. van Halbeek. |
