ProGP148 (β-1,4-mannanase (G-enzyme))
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| ProGP ID | ProGP148 (β-1,4-mannanase (G-enzyme)) |
| Validation Status | Uncharacterized |
| Organism Information | |
| Organism Name | Bacillus circulans K-1 |
| Domain | Bacteria |
| Taxonomic ID (NCBI) | 1397 |
| Genome Information | |
| GenBank | AB007123 |
| EMBL | AB007123 |
| Gene Information | |
| Gene Name | G-gene |
| Protein Information | |
| Protein Name | β-1,4-mannanase (G-enzyme) |
| UniProtKB/SwissProt ID | O66185 |
| EMBL-CDS | BAA25878.1 |
| UniProtKB Sequence | >tr|O66185|O66185_BACCI Mannanase OS=Bacillus circulans PE=4 SV=1 MGWFLVILRKWLIAFVAFLLMFSWTGQLTNKAHAASGFYVSGTKLLDATGQPFVMRGVNH AHTWYKDQLSTAIPAIAKTGANTIRIVLANGHKWTLDDVNTVNNILTLCEQNKLIAVLEV HDATGSDSLSDLDNAVNYWIGIKSALIGKEDRVIINIANEWYGTWDGVAWANGYKQAIPK LRNAGLTHTLIVDSAGWGQYPDSVKNYGTEVLNADPLKNTVFSIHMYEYAGGNASTVKSN IDGVLNKNLALIIGEFGGQHTNGDVDEATIMSYSQEKGVGWLAWSWKGNSSDLAYLDMTN DWAGNSLTSFGNTVVNGSNGIKATSVLSGIFGGVTPTSSPTSTPTSTPTSTPTPTPSPTP SPGNNGTILYDFETGTQGWSGNNISGGPWVTNEWKATGAQTLKADVSLQSNSTHSLYITS NQNLSGKSSLKATVKHANWGNIGNGIYAKLYVKTGSGWTWYDSGENLIQSNDGTILTLSL SGISNLSSVKEIGVEFRASSNSSGQSAIYVDSVSLQ |
| Sequence length | 516 AA |
| Subcellular Location | Intracellular |
| Function | Degrades guar gum which consists of a galactomannosaccharide from the endosperm of guar seeds. |
| Glycosylation Status | |
| Technique(s) used for Glycosylation Detection | Mass shift detected on SDS-polyacrylamide gel, periodic acid-Schiff (PAS) staining |
| Glycan Information | |
| Glycan Annotation | Recombinant G-enzyme (expressed in E. coli) shows GalNAc, Xyl, GlcNAc, Man, Glc, and Gal to account for 1.7%, 14.4%, 6.1%, 3.2%, 54.2%, and 10.4%, respectively, of total monosaccharides. G-enzyme from strain K-1 contains GalNAc, Xyl, GlcNAc, Man, Fuc, and Gal that account for 1.8%, 0.9%, 11.0%, 6.1%, 1.1%, and 79.1%, respectively, of total monosaccharides. |
| Literature | |
| Year of Identification | 1998 |
| Year of Identification Month Wise | 1998.03 |
| Reference | Yoshida, S., Sako, Y. and Uchida, A., 1998. Cloning, sequence analysis, and expression in Escherichia coli of a gene coding for an enzyme from Bacillus circulans K-1 that degrades guar gum. Bioscience, biotechnology, and biochemistry, 62(3), pp.514-520. |