ProGP149 (Crystal toxin (66 kDa))
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| ProGP ID | ProGP149 (Crystal toxin (66 kDa)) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Bacillus thuringiensis subsp. kurstaki |
| Domain | Bacteria |
| Classification | Phylum : Firmicutes Class : Clostridia Orders : bacillales Family : Bacillaceae Genus : Bacillus Species : thuringiensis Subspecies : kurstaki |
| Taxonomic ID (NCBI) | 1428 |
| Genome Information | |
| GenBank | NZ_MSFC01000028.1. |
| EMBL | CP012099 |
| Protein Information | |
| Protein Name | Crystal toxin (66 kDa) |
| Subcellular Location | Membrane associated |
| Function | Insecticidal protein. |
| Glycosylation Status | |
| Glycosylation Type | O- (Thr) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | T36 and T37 |
| Technique(s) used for Glycosylation Detection | ConA-Sepharose affinity chromatography and DIG Glycan Differentiation Kit |
| Glycan Information | |
| Glycan Annotation | 5–6% carbohydrate content, with 20 glucose and mannose, per subunit. 250 pg neutral sugars per mg protein. "High-mannose" or "hybrid-type N-glycosidically-linked carbohydrate chains. Presence of terminal mannose, α( 1-3), α(1-6) or α( 1-2) linked to mannose. |
| Literature | |
| Year of Identification | 1998 |
| Year of Identification Month Wise | 1998.04 |
| Reference | Suresh Kumar, N., Reddy, S.T. and Venkateswerlu, G., 1998. Effect of sodium dodecyl sulfate on concanavalin A-sepharose during affinity chromatography of high-affinity binding toxin protein of Bacillus thuringiensis subsp. kurstaki. Analytical letters, 31(10), pp.1677-1687. |
| Corresponding Author | G Venkateswerlu |
| Contact | Department of biochemistry osmania university Hyderabad |