ProGP149 (Crystal toxin (66 kDa))
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ProGP ID | ProGP149 (Crystal toxin (66 kDa)) |
Validation Status | Characterized |
Organism Information | |
Organism Name | Bacillus thuringiensis subsp. kurstaki |
Domain | Bacteria |
Classification | Family: Bacillaceae Order: Bacillales Class: Bacilli (or Firmibacteria) Division or phylum: "Firmicutes" |
Taxonomic ID (NCBI) | 1428 |
Genome Information | |
GenBank | NZ_MSFC01000028.1. |
EMBL | CP012099 |
Protein Information | |
Protein Name | Crystal toxin (66 kDa) |
Subcellular Location | Membrane associated |
Function | Insecticidal protein. |
Glycosylation Status | |
Glycosylation Type | O- (Thr) linked |
Experimentally Validated Glycosite(s) in Full Length Protein | T36 and T37 |
Technique(s) used for Glycosylation Detection | ConA-Sepharose affinity chromatography and DIG Glycan Differentiation Kit |
Glycan Information | |
Glycan Annotation | 5–6% carbohydrate content, with 20 glucose and mannose, per subunit. 250 pg neutral sugars per mg protein. "High-mannose" or "hybrid-type N-glycosidically-linked carbohydrate chains. Presence of terminal mannose, ?( 1-3), ?(1-6) or ?( 1-2) linked to mannose. |
Literature | |
Year of Identification | 1998 |
Year of Identification Month Wise | 1998.04 |
Reference | Kumar, N.S., Reddy, S.T. and Venkateswerlu, G. (1998) Effect of sodium dodecyl sulfate on concanavalin A-sepharose during Affinity chromatography of high-affinity binding toxin protein of Bacillus thuringiensis subsp. kurstaki. Anal Lett, 31, 1677-1687. |
Author | Kumar, N.S., Reddy, S.T. Venkateswerlu, G. |
Research Group | Department of biochemistry osmania university Hyderabad |
Corresponding Author | Venkateswerlu, G. |
Contact | Department of biochemistry osmania university Hyderabad |