Membrane residing ectoenzyme. Due to the likely pseudoperiplasmic location, cytochrome c-like function is proposed analogous to the situation in Paracoccus denitrificans linking pseudoperiplasmic redox metabolism to membrane-residing electron transport systems. Cytochrome b558/566 is significantly up-regulated under certain organotrophic growth conditions in combination with low oxygen tension.
Glycosylation Status
Glycosylation Type
N- (Asn) linked, (O- linked residues not yet identified)
Experimentally Validated Glycosite(s) in Full Length Protein
N144, N164
Experimentally Validated Glycosite(s ) in Mature Protein
Identification of oligosaccharides using Gel Chromatography and GLC-MS (PNGase F treatment followed by HPAEC analysis). Glycan structure is elucidated with the help of 1H-NMR spectrum from two-dimensional 1H, 1H correlation NMR Spectroscopy . Linkage analysis was carried out using 13C NMR Chemical Shift analysis.
Provides first extensive study and a structure for oligosaccharide attached to a crenarchaeotal glycoprotein. The protein attached hexasaccharide is unique in terms of the presence of a trisubstituted N acetylglucosamine residue and a rare acidic residue Qui6S (sulfonated sugar). Qui6S like sugar has previously been observed only in glycolipids of chloroplast and photosynthetic bacteria. Only other case where sufated sugar has been observed is H. salinaium. Unusally, only one type of glycan has been found associated with cytochrome b558/566 subunit A.
