ProGP167 (BclA (collagen-like protein))
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| ProGP ID | ProGP167 (BclA (collagen-like protein)) |
| Validation Status | Uncharacterized |
| Organism Information | |
| Organism Name | Bacillus cereus ATCC 10876 |
| Domain | Bacteria |
| Classification | Phylum : Firmicutes Class : Bacilli Orders : Bacillales Family : Bacillaceae Genus : Bacillus Species : cereus |
| Taxonomic ID (NCBI) | 1396 |
| Genome Information | |
| GenBank | NZ_MNKZ01000327.1. |
| EMBL | AF246294 |
| Protein Information | |
| Protein Name | BclA (collagen-like protein) |
| Subcellular Location | Surface |
| Function | 1. 205,70 and 30-kDa glycoproteins are present in the exosporium which is a transparent and loose covering of the spore. 2. BclA is the major constituent of the hairy surface of exosporium, the resistant and flexible paracrystalline layer of spores. The exosporium plays a role in spore adhesion and virulence. The trimerized glycoprotein BclA is attached to the basal layer through an N-terminal domain. |
| Glycosylation Status | |
| Technique(s) used for Glycosylation Detection | Glycoprotein detection kit |
| Glycan Information | |
| Glycan Annotation | Sugars identified include glucose, rhamnose, ribose, and glucosamine (amino sugar). 3.8% total amino sugar content determined. In Bacillus cereus strain T, carbohydrates were determined using differential anthrone method, orcinol method, and aniline phthalic acid and ammoniacal silver nitrate reactions after descending paper chromatography. |
| Literature | |
| Year of Identification | 1999 |
| Year of Identification Month Wise | 1999.1 |
| Reference | Todd, S.J., Moir, A.J., Johnson, M.J. and Moir, A., 2003. Genes of Bacillus cereus and Bacillus anthracis encoding proteins of the exosporium. Journal of bacteriology, 185(11), pp.3373-3378. |
| Corresponding Author | Anne Moir |
| Contact | Department of Molecular Biology and Biotechnology, University of Sheffield, United Kingdom. |
| Reference | Charlton, S., Moir, A.J.G., Baillie, L. and Moir, A., 1999. Characterization of the exosporium of Bacillus cereus. Journal of applied microbiology, 87(2), pp.241-245. |
| Corresponding Author | Anne Moir |
| Contact | Department of Molecular Biology and Biotechnology, University of Sheffield, United Kingdom. |
| Reference | Matz, L.L., Beaman, T.C. and Gerhardt, P., 1970. Chemical composition of exosporium from spores of Bacillus cereus. Journal of bacteriology, 101(1), pp.196-201. |