| Technique(s) used for Glycosylation Detection | Periodic acid-Schiff (PAS) staining and carbohydrate content determination using orcinol-sulfuric acid assay |
| Technique(s) used for Glycosylated Residue(s) Detection | Edman degradation (of glycopeptides and deglycosylated peptides) and nano-ESI-QTOF-MS/MS (electrospray ionization-quadrupole time of flight tandem mass spectrometry) |
| Glycan Information |
| Glycan Annotation | Linkage: ?-D-Galp-Ser/Thr.
[?2)-?-L-Rhap-(1?3)-?-L-Rhap-(1?2)-?-L-Rhap-(1?]n =13–18 (15 on average), with a 2-Omethyl group capping the terminal trisaccharide repeating unit at the non-reducing end of the glycan chains. The glycan chains are bound via the disaccharide (or trisaccharide with additional ?-L-Rhap) core?3)-?-L-Rhap-(1?3)-?-L-Rhap-(1?and the linkage glycose ?-D-Galp. Approx. 3.6% sugar content detected. Three inherently heterogeneic glycoprotein species of SgsE, 101.66 kDa, 108.68 kDa, and 115.73 kDa, have been determined. Nanoheterogeneity has been assigned to the S-layer glycans, with the most prevalent variation between 12 and 18 trisaccharide repeating units. One, two and three glycosylation sites, respectively, are occupied in these three glycoprotein species. |
| BCSDB ID | 22701 |
| GlyTouCan | G26252RJ |
| Technique(s) used for Glycan Identification | HPAEC/PAD (high performance anion-exchange chromatography/pulsed amperometric detection), MALDI-TOF MS analysis, 1D and 2D 1H and 13C NMR spectroscopy including 2D NOESY (nuclear Overhauser enhancement spectroscopy) and HMBC (heteronuclear multiple bond correlation spectroscopy) |
| Literature |
| Year of Identification | 1984 |
| Year of Identification Month Wise | 1984 |
| Year of Validation | 2002 |
| Reference | Messner, P., Steiner, K., Zarschler, K. and Schaffer, C. (2008) S-layer nanoglycobiology of bacteria. Carbohydr Res, 343, 1934-1951. [PubMed: 18336801] |
| Author | Messner, P., Steiner, K., Zarschler, K. and Schaffer, C. |
| Research Group | University of Natural Resources and Applied Life Sciences, Vienna, Center for NanoBiotechnology A-1180 Vienna, Gregor-Mendel-Strasse 33, Austria. |
| Corresponding Author | Schaffer, C |
| Contact | University of Natural Resources and Applied Life Sciences, Vienna, Center for NanoBiotechnology A-1180 Vienna, Gregor-Mendel-Strasse 33, Austria. |
| Reference | Steiner, K., Novotny, R., Werz, D.B., Zarschler, K., Seeberger, P.H., Hofinger, A., Kosma, P., Schaffer, C. and Messner, P. (2008) Molecular basis of S-layer glycoprotein glycan biosynthesis in Geobacillus stearothermophilus. J Biol Chem, 283, 21120-21133. [PubMed: 18515358] |
| Author | Steiner, K., Novotny, R., Werz, D.B., Zarschler, K., Seeberger, P.H., Hofinger, A., Kosma, P., Schaffer, C. Messner, P. |
| Research Group | Center for NanoBiotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria. |
| Corresponding Author | Messner, P. |
| Contact | Center for NanoBiotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria. |
| Reference | Steiner, K., Novotny, R., Werz, D.B., Zarschler, K., Seeberger, P.H., Hofinger, A., Kosma, P., Schaffer, C. and Messner, P. (2008) Molecular basis of S-layer glycoprotein glycan biosynthesis in Geobacillus stearothermophilus. J Biol Chem, 283, 21120-21133. [PubMed: 18515358] |
| Author | Steiner, K., Novotny, R., Werz, D.B., Zarschler, K., Seeberger, P.H., Hofinger, A., Kosma, P., Schaffer, C. Messner, P. |
| Research Group | Center for NanoBiotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria. |
| Corresponding Author | Messner, P. |
| Contact | Center for NanoBiotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria. |
| Reference | Steiner, K., Novotny, R., Patel, K., Vinogradov, E., Whitfield, C., Valvano, M.A., Messner, P. and Schaffer, C. (2007) Functional characterization of the initiation enzyme of S-layer glycoprotein glycan biosynthesis in Geobacillus stearothermophilus NRS 2004/3a. J Bacteriol, 189, 2590-2598. [PubMed: 17237178] |
| Author | Steiner, K., Novotny, R., Patel, K., Vinogradov, E., Whitfield, C., Valvano, M.A., Messner, P. Schaffer, C. |
| Research Group | Center for NanoBiotechnology, University of Natural Resources and Applied Life Sciences, Vienna, A-1180 Vienna, Austria |
| Corresponding Author | Schaffer, C. |
| Contact | Center for NanoBiotechnology, University of Natural Resources and Applied Life Sciences, Vienna, A-1180 Vienna, Austria |
| Reference | Steiner, K., Pohlentz, G., Dreisewerd, K., Berkenkamp, S., Messner, P., Peter-Katalinic, J. and Schaffer, C. (2006) New insights into the glycosylation of the surface layer protein SgsE from Geobacillus stearothermophilus NRS 2004/3a. J Bacteriol, 188, 7914-7921. [PubMed: 16963578] |
| Author | Steiner, K., Pohlentz, G., Dreisewerd, K., Berkenkamp, S., Messner, P., Peter-Katalinic, J. Schaffer, C. |
| Research Group | Edinburgh, UK. |
| Corresponding Author | Schaffer, C. |
| Contact | Edinburgh, UK. |
| Reference | Novotny, R., Schaffer, C., Strauss, J. and Messner, P. (2004) S-layer glycan-specific loci on the chromosome of Geobacillus stearothermophilus NRS 2004/3a and dTDP-L-rhamnose biosynthesis potential of G. stearothermophilus strains. Microbiology, 150, 953-965. [PubMed: 15073305] |
| Author | Novotny, R., Schaffer, C., Strauss, J. Messner, P. |
| Research Group | Center for NanoBiotechnology, University of Applied Life Sciences and Natural Resources, A-1180 Vienna, Austria. |
| Corresponding Author | Messner, P. |
| Contact | Center for NanoBiotechnology, University of Applied Life Sciences and Natural Resources, A-1180 Vienna, Austria. |
| Reference | Schaffer, C., Wugeditsch, T., Kahlig, H., Scheberl, A., Zayni, S. and Messner, P. (2002) The surface layer (S-layer) glycoprotein of Geobacillus stearothermophilus NRS 2004/3a. Analysis of its glycosylation. J Biol Chem, 277, 6230-6239. [PubMed: 11741945] |
| Author | Schaffer, C., Wugeditsch, T., Kahlig, H., Scheberl, A., Zayni, S. and Messner, P. |
| Research Group | Center for Ultrastructure Research and Ludwig Boltzmann Institute for Molecular Nanotechnology, University of Natural Resources and Applied Life Sciences, Vienna, A-1180 Vienna, Austria. |
| Corresponding Author | Messner, P |
| Contact | Center for Ultrastructure Research and Ludwig Boltzmann Institute for Molecular Nanotechnology, University of Natural Resources and Applied Life Sciences, Vienna, A-1180 Vienna, Austria. |
| Reference | Kupcu, Z., Marz, L., Messner, P. and Sleytr, U.B. (1984) Evidence for the glycoprotein nature of the crystalline cell wall surface layer of Bacillus stearothermophilus strain NRS2004/3a. FEBS Lett, 173, 185-190. [PubMed: 6745426] |
| Author | Kupcu, Z., Marz, L., Messner, P. and Sleytr, U.B. |
| Corresponding Author | Sleytr, U.B. |
