| Technique(s) used for Glycosylation Detection | Aberrant mobility on SDS-polyacrylamide gel, labelling with digoxigenin (DIG) hydrazide after periodate oxidation. The glycan was not detectable by common lectins and could not be deglycosylated by a broad spectrum of glycosidases. |
| Technique(s) used for Glycosylated Residue(s) Detection | MS-MS (tandem mass spectrometry) and N-terminal sequencing of AIDA-I peptides |
| Protein Glycosylation- Implication | Glycosylation is required to ensure a normal conformation of AIDA-I. Glycans could be involved in receptor recognition making it essential for adhesion but not for autoaggregation or biofilm formation. It also provides increased resistance to degradation. Only the glycosylated form binds to cultured epithelial cells. Further unglycosylated AIDA-I is expressed in smaller amounts than its relatively thermostable glycosylated form. Unglycosylated AIDA-I is more sensitive to proteases and induces important extracytoplasmic stress. |
| Glycan Information |
| Glycan Annotation | Heterogenous multiple heptose residues (atleast 19 per protein molecule). |
| Technique(s) used for Glycan Identification | Gas chromatography and mass spectrometry after methanolysis and carbohydrate labelling. |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | Aah is the heptosyltransferase. Tib C (E. coli (ETEC) strain H10407) has sequence similarity with Aah gene and it can substitute function of Aah. |
| OST ProGT ID | ProGT8 (Aah) |
| Literature |
| Reference | Charbonneau, M.È., Girard, V., Nikolakakis, A., Campos, M., Berthiaume, F., Dumas, F., Lépine, F. and Mourez, M., 2007. O-linked glycosylation ensures the normal conformation of the autotransporter adhesin involved in diffuse adherence. Journal of bacteriology, 189(24), pp.8880-8889. |
| Corresponding Author | Michael Mourez |
| Contact | Canada Research Chair on Bacterial Animal Diseases, University of Montreal, Faculty of Veterinary Medicine, St.-Hyacinthe, 3200 Sicotte, St.-Hyacinthe, Quebec J2S 7C6, Canada |
| Reference | Sherlock, O., Schembri, M.A., Reisner, A. and Klemm, P., 2004. Novel roles for the AIDA adhesin from diarrheagenic Escherichia coli: cell aggregation and biofilm formation. Journal of bacteriology, 186(23), pp.8058-8065. |
| Corresponding Author | Per Klemm |
| Contact | Centre for Biomedical Microbiology, BioCentrum-DTU, Technical University of Denmark, DK-2800 Lyngby, Denmark. |
| Reference | Moormann, C., Benz, I. and Schmidt, M.A., 2002. Functional substitution of the TibC protein of enterotoxigenic Escherichia coli strains for the autotransporter adhesin heptosyltransferase of the AIDA system. Infection and immunity, 70(5), pp.2264-2270. |
| Corresponding Author | M. Alexander Schmidt |
| Contact | Institute of Infectiology, Center for Molecular Biology of Inflammation, Westfälische Wilhelms-Universität Münster, D-48149 Münster, Germany |
| Reference | Benz, I. and Schmidt, M.A., 2001. Glycosylation with heptose residues mediated by the aah gene product is essential for adherence of the AIDA‐I adhesin. Molecular microbiology, 40(6), pp.1403-1413. |
| Corresponding Author | M. Alexander Schmidt |
| Contact | Institute of Infectiology, Center for Molecular Biology of Inflammation, Westfälische Wilhelms-Universität Münster, D-48149 Münster, Germany |
| Reference | Charbonneau, M.E., Girard, V., Nikolakakis, A., Campos, M., Berthiaume, F., Dumas, F., Lepine, F. and Mourez, M. (2007) O-linked glycosylation ensures the normal conformation of the autotransporter adhesin involved in diffuse adherence. J Bacteriol, 189, 8880-8889. [PubMed: 17951390] |
| Author | Charbonneau, M.E., Girard, V., Nikolakakis, A., Campos, M., Berthiaume, F., Dumas, F., Lepine, F. and Mourez, M. |
| Research Group | University of Montreal, Faculty of Veterinary Medicine, 3200 Sicotte, St.-Hyacinthe, Quebec J2S 7C6, Canada. |
| Corresponding Author | Mourez, M. |
| Contact | University of Montreal, Faculty of Veterinary Medicine, 3200 Sicotte, St.-Hyacinthe, Quebec J2S 7C6, Canada. |
| Reference | Sherlock, O., Schembri, M.A., Reisner, A. and Klemm, P. (2004) Novel roles for the AIDA adhesin from diarrheagenic Escherichia coli: cell aggregation and biofilm formation. J Bacteriol, 186, 8058-8065. [PubMed: 15547278] |
| Author | Sherlock, O., Schembri, M.A., Reisner, A. Klemm, P. |
| Research Group | Centre for Biomedical Microbiology, BioCentrum-DTU, Bldg. 301, Technical University of Denmark, DK-2800 Lyngby, Denmark. |
| Corresponding Author | Klemm, P. |
| Contact | Centre for Biomedical Microbiology, BioCentrum-DTU, Bldg. 301, Technical University of Denmark, DK-2800 Lyngby, Denmark. |
| Reference | Sherlock, O., Schembri, M.A., Reisner, A. and Klemm, P. (2004) Novel roles for the AIDA adhesin from diarrheagenic Escherichia coli: cell aggregation and biofilm formation. J Bacteriol, 186, 8058-8065. [PubMed: 15547278] |
| Author | Sherlock, O., Schembri, M.A., Reisner, A. Klemm, P. |
| Research Group | Centre for Biomedical Microbiology, BioCentrum-DTU, Technical University of Denmark, DK-2800 Lyngby, Denmark. |
| Corresponding Author | Klemm, P. |
| Contact | Centre for Biomedical Microbiology, BioCentrum-DTU, Technical University of Denmark, DK-2800 Lyngby, Denmark. |
| Reference | Moormann, C., Benz, I. and Schmidt, M.A. (2002) Functional substitution of the TibC protein of enterotoxigenic Escherichia coli strains for the autotransporter adhesin heptosyltransferase of the AIDA system. Infect Immun, 70, 2264-2270. [PubMed: 11953358] |
| Author | Moormann, C., Benz, I. Schmidt, M.A. |
| Research Group | Institute of Infectiology, Center for Molecular Biology of Inflammation, Westfälische Wilhelms-University Münster, D-48149 Münster, Germany. |
| Corresponding Author | Schmidt, M.A. |
| Contact | Institute of Infectiology, Center for Molecular Biology of Inflammation, Westfälische Wilhelms-University Münster, D-48149 Münster, Germany. |
| Reference | Benz, I. and Schmidt, M.A. (2001) Glycosylation with heptose residues mediated by the aah gene product is essential for adherence of the AIDA-I adhesin. Mol Microbiol, 40, 1403-1413. [PubMed: 11442838] |
| Author | Benz, I. Schmidt, M.A. |
| Research Group | nstitute of Infectiology - Center for Molecular Biology of Inflammation (ZMBE), University Hospital Münster, Germany. |
| Corresponding Author | Schmidt, M.A. |
| Contact | nstitute of Infectiology - Center for Molecular Biology of Inflammation (ZMBE), University Hospital Münster, Germany. |
