ProGP208 (SbsD (S-layer glycoprotein))
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| ProGP ID | ProGP208 (SbsD (S-layer glycoprotein)) |
| Validation Status | Uncharacterized |
| Organism Information | |
| Organism Name | Geobacillus (Bacillus) stearothermophilus ATCC 12980/G+ |
| Domain | Bacteria |
| Classification | Phylum : Firmicutes Class : Bacilli Orders : Bacillales Family : Bacillaceae Genus : Geobacillus Species : stearothermphilus Strain : ATCC 12980/G+ |
| Taxonomic ID (NCBI) | 1422 |
| Genome Information | |
| GenBank | AF228338 |
| EMBL | AF228338 |
| Gene Information | |
| Gene Name | sbsD |
| Protein Information | |
| Protein Name | SbsD (S-layer glycoprotein) |
| UniProtKB/SwissProt ID | Q9KIQ5 |
| EMBL-CDS | AAF34763.2 |
| UniProtKB Sequence | >tr|Q9KIQ5|Q9KIQ5_GEOSE SbsD OS=Geobacillus stearothermophilus GN=sbsD PE=4 SV=1 MDKKKAVKLATASAVAASAFVAANPHASQAATDVATVVSQAKAQMKEAYYTYSHTVTETG KLPNISDVYAAYNKAKQAYANAVAVVNKAGGAKKDAYLADLQATYETYVFKANPKSGEAR VATYIDAYNYATKLDAMRQELKAAVEAKDLKKAEELYHKISYELKTRTVILDRVYGQSTR ELLRSTFKADAQALRDSLIYDITVAMKAREAQDAAKAGNLDKAKAALDQVNQYVSKVTDA FKAELQKAAQDANAAYEAALPPKVESVTAVNAKTLEIKFNKAVDVATVIDNKGTSDTSDD VVKATAITLTAIDGQGSVSTVKASLSDDKKTLKLVADGSQFFTKRYVVDIKNVKTLDGKD VPSYTTTIDTTDSVRPSVLSSSYADNGLTLKVKFSEPLASVGTVKLYDGTTEISVSPKFT AGDDEMTINLASSSVPVNKDLTLKIFGAVDYNGNVINPNPAELTVKKTTVDTTKPTVQNI EAVNTKTVKVTFSEKLLSNPTIKIGGQTASVSVDSTGLVYTATLANALSEGVYAVEVSDY KDLAGNAGDTYTKVVQLKADTTAPKFVSSQVVKIDGVEHLVLTFDEEVTTGSNITVVQAS DKYVDENNVLKSVGTTLTTTSDNFKLYLPTDGKSKSVALNISSLPKGTYTVTLPDRLVSD LAGNPYAERKQITFVRGSDSLTTKPALDSSYDNNGVKADNNNELVFAFTQNLDASALNLS NFNINGLAVTKAVFDGDTKHIRVTLAPGANTWTGTHVITISNIKNTSGLVMDTVTVSEFM KENVAPTFTATLTSADVIRVDFSEPVANATISSPLSANNFTVKVDGNVVTVSNVYEDNNA TNQVQGSTGYKTVYLKLQSPVSDLSKPITLSATGIVDVDQTGAITNNNLVGNNVSNAVVN VAK |
| Sequence length | 903 AA |
| Subcellular Location | Surface |
| Function | Biosynthesis of S-layer glycoprotein occurs due to S-layer variation at higher temperature. |
| Glycosylation Status | |
| Glycosylation Type | O- (Ser/Thr) linked |
| Technique(s) used for Glycosylation Detection | Periodate-Schiff (PAS) reagent staining, deglycosylation with trifluoromethanesulfonic acid |
| Glycan Information | |
| Glycan Annotation | Contains mannose, glucose, galactose and rhamnose as revealed by neutral sugar and amino sugar analysis. These sugars are present in a molar ratio of 1.85, 1, 1.02 and 0.78, respectively. Glycan chains are bound via O-linkage to serine or threonine residues. |
| Literature | |
| Year of Identification | 2001 |
| Year of Identification Month Wise | 2001.12 |
| Reference | Egelseer, E.M., Danhorn, T., Pleschberger, M., Hotzy, C., Sleytr, U.B. and Sára, M., 2001. Characterization of an S-layer glycoprotein produced in the course of S-layer variation of Bacillus stearothermophilus ATCC 12980 and sequencing and cloning of the sbsD gene encoding the protein moiety. Archives of microbiology, 177(1), pp.70-80. |
| Corresponding Author | Eva M. Egelseer |
| Contact | Center for Ultrastructure Research, Ludwig Boltzmann-Institute for Molecular Nanotechnology, University of Agricultural Sciences, 1180 Vienna |
