| Technique(s) used for Glycosylation Detection | DIG Glycan/Protein Double Labelling (DIG-hydrazide labeling after periodate oxidation) followed by Western blot using anti-DIG antibodies. |
| Technique(s) used for Glycosylated Residue(s) Detection | cLC/ESMS (capillary liquid chromatography electrospray mass spectrometry) and MS/MS (tandem mass spectrometry) after β-elimination with ammonium hydroxide |
| Glycan Information |
| Glycan Annotation | Pseudaminic acid (Pse5Ac7Ac, 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-nonulosonic acid). A total of seven O-linked Pse5Ac7Ac residues are present in FlaA protein. |
| BCSDB ID | 4618 |
| GlyTouCan | G75457NU |
| Technique(s) used for Glycan Identification | CE-ESMS (capillary electrophoresis-electrospray mass spectrometry), tandem mass spectrometry and precursor ion scanning with fe-CID (front-end collision-induced dissociation) |
| Literature |
| Year of Identification | 2002 |
| Year of Identification Month Wise | 2002.6.1 |
| Year of Validation | 2003 |
| Reference | Schoenhofen, I.C., Lunin, V.V., Julien, J.P., Li, Y., Ajamian, E., Matte, A., Cygler, M., Brisson, J.R., Aubry, A., Logan, S.M. and Bhatia, S., 2006. Structural and functional characterization of PseC, an aminotransferase involved in the biosynthesis of pseudaminic acid, an essential flagellar modification in Helicobacter pylori. Journal of Biological Chemistry, 281(13), pp.8907-8916. |
| Corresponding Author | N. Martin Young |
| Contact | Institute for Biological Sciences, National Research Council, Ottawa, Ontario K1A 0R6. |
| Reference | Schirm, M., Soo, E.C., Aubry, A.J., Austin, J., Thibault, P. and Logan, S.M., 2003. Structural, genetic and functional characterization of the flagellin glycosylation process in Helicobacter pylori. Molecular microbiology, 48(6), pp.1579-1592. |
| Corresponding Author | Susan M Logan |
| Contact | Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario K1A 0R6, Canada. |
| Reference | Josenhans, C., Vossebein, L., Friedrich, S. and Suerbaum, S., 2002. The neuA/flmD gene cluster of Helicobacter pylori is involved in flagellar biosynthesis and flagellin glycosylation. FEMS microbiology letters, 210(2), pp.165-172. |
| Corresponding Author | Christine Josenhans |
| Contact | Institute for Hygiene and Microbiology at the University of Würzburg, Josef-Schneider-Strasse 2, D-97080 Würzburg, Germany |
| Reference | Schoenhofen, I.C., Lunin, V.V., Julien, J.P., Li, Y., Ajamian, E., Matte, A., Cygler, M., Brisson, J.R., Aubry, A., Logan, S.M. et al. (2006) Structural and functional characterization of PseC, an aminotransferase involved in the biosynthesis of pseudaminic acid, an essential flagellar modification in Helicobacter pylori. J Biol Chem, 281, 8907-8916. [PubMed: 16421095] |
| Author | Schoenhofen, I.C., Lunin, V.V., Julien, J.P., Li, Y., Ajamian, E., Matte, A., Cygler, M., Brisson, J.R., Aubry, A., Logan, S.M. |
| Research Group | Institute for Biological Sciences, National Research Council, Ottawa, Ontario K1A 0R6 |
| Corresponding Author | Logan, S.M. |
| Contact | Institute for Biological Sciences, National Research Council, Ottawa, Ontario K1A 0R6 |
| Reference | Schirm, M., Soo, E.C., Aubry, A.J., Austin, J., Thibault, P. and Logan, S.M. (2003) Structural, genetic and functional characterization of the flagellin glycosylation process in Helicobacter pylori. Mol Microbiol, 48, 1579-1592. [PubMed: 12791140] |
| Author | Schirm, M., Soo, E.C., Aubry, A.J., Austin, J., Thibault, P. Logan, S.M. |
| Research Group | University of Montreal, Department of Chemistry, Canada. |
| Corresponding Author | Logan, S.M. |
| Contact | University of Montreal, Department of Chemistry, Canada. |
| Reference | Josenhans, C., Vossebein, L., Friedrich, S. and Suerbaum, S. (2002) The neuA/flmD gene cluster of Helicobacter pylori is involved in flagellar biosynthesis and flagellin glycosylation. FEMS Microbiol Lett, 210, 165-172. [PubMed: 12044670] |
| Author | Josenhans, C., Vossebein, L., Friedrich, S. Suerbaum, S. |
| Research Group | Institute of Hygiene and Microbiology, University of Würzburg, Josef-Schneider-Strasse 2, D-97080 Würzburg, Germany. |
| Corresponding Author | Suerbaum, S. |
| Contact | Institute of Hygiene and Microbiology, University of Würzburg, Josef-Schneider-Strasse 2, D-97080 Würzburg, Germany. |
