| Technique(s) used for Glycosylation Detection | DIG Glycan/Protein Double Labelling (DIG-hydrazide labeling after periodate oxidation) followed by Western blot using anti-DIG antibodies. |
| Technique(s) used for Glycosylated Residue(s) Detection | cLC/ESMS (capillary liquid chromatography electrospray mass spectrometry) and MS/MS (tandem mass spectrometry) after β-elimination with ammonium hydroxide |
| Glycan Information |
| Glycan Annotation | Pseudaminic acid (Pse5Ac7Ac, 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-nonulosonic acid). A total of seven O-linked Pse5Ac7Ac residues are present in FlaA protein. |
| BCSDB ID | 4618 |
| GlyTouCan | G75457NU |
| Technique(s) used for Glycan Identification | CE-ESMS (capillary electrophoresis-electrospray mass spectrometry), tandem mass spectrometry and precursor ion scanning with fe-CID (front-end collision-induced dissociation) |
| Literature |
| Year of Identification | 2002 |
| Year of Identification Month Wise | 2002.6.1 |
| Year of Validation | 2003 |
| Reference | Schoenhofen, I.C., Lunin, V.V., Julien, J.P., Li, Y., Ajamian, E., Matte, A., Cygler, M., Brisson, J.R., Aubry, A., Logan, S.M. et al. (2006) Structural and functional characterization of PseC, an aminotransferase involved in the biosynthesis of pseudaminic acid, an essential flagellar modification in Helicobacter pylori. J Biol Chem, 281, 8907-8916. [PubMed: 16421095] |
| Author | Schoenhofen, I.C., Lunin, V.V., Julien, J.P., Li, Y., Ajamian, E., Matte, A., Cygler, M., Brisson, J.R., Aubry, A., Logan, S.M. |
| Research Group | Institute for Biological Sciences, National Research Council, Ottawa, Ontario K1A 0R6 |
| Corresponding Author | Logan, S.M. |
| Contact | Institute for Biological Sciences, National Research Council, Ottawa, Ontario K1A 0R6 |
| Reference | Schirm, M., Soo, E.C., Aubry, A.J., Austin, J., Thibault, P. and Logan, S.M. (2003) Structural, genetic and functional characterization of the flagellin glycosylation process in Helicobacter pylori. Mol Microbiol, 48, 1579-1592. [PubMed: 12791140] |
| Author | Schirm, M., Soo, E.C., Aubry, A.J., Austin, J., Thibault, P. Logan, S.M. |
| Research Group | University of Montreal, Department of Chemistry, Canada. |
| Corresponding Author | Logan, S.M. |
| Contact | University of Montreal, Department of Chemistry, Canada. |
| Reference | Josenhans, C., Vossebein, L., Friedrich, S. and Suerbaum, S. (2002) The neuA/flmD gene cluster of Helicobacter pylori is involved in flagellar biosynthesis and flagellin glycosylation. FEMS Microbiol Lett, 210, 165-172. [PubMed: 12044670] |
| Author | Josenhans, C., Vossebein, L., Friedrich, S. Suerbaum, S. |
| Research Group | Institute of Hygiene and Microbiology, University of Würzburg, Josef-Schneider-Strasse 2, D-97080 Würzburg, Germany. |
| Corresponding Author | Suerbaum, S. |
| Contact | Institute of Hygiene and Microbiology, University of Würzburg, Josef-Schneider-Strasse 2, D-97080 Würzburg, Germany. |
