| Technique(s) used for Glycosylation Detection | Digoxygenin (DIG)-glycan detection |
| Technique(s) used for Glycosylated Residue(s) Detection | MS-MS (tandem mass spectrometry) |
| Protein Glycosylation- Implication | Glycosylation protects HMW1 against premature degradation during the process of secretion and facilitates HMW1 tethering to the bacterial surface, a prerequisite for HMW1-mediated adherence. |
| Glycan Information |
| Glycan Annotation | Unusual carbohydrate modification includes glucose, galactose, and possibly mannose and corresponds to 7?8 kDa of the molecular mass. 31 modification sites carry 47 hexose units indicating the presence of hexose and dihexose (162-Da) sugars. HMW1C is capable of transferring glucose and galactose to HMW1 and is also able to generate hexose-hexose bonds. |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | HMW1C (ApHMW1C Actinobacillus pleuropneumoniae) |
| OST ProGT ID | ProGT39 (HMW1C) |
| Literature |
| Reference | Choi, K.J., Grass, S., Paek, S., St. Geme III, J.W. and Yeo, H.J., 2010. The Actinobacillus pleuropneumoniae HMW1C-like glycosyltransferase mediates N-linked glycosylation of the Haemophilus influenzae HMW1 adhesin. PLoS One, 5(12), p.e15888. |
| Corresponding Author | Hye-Jeong Yeo |
| Contact | Department of Biology and Biochemistry, University of Houston, Houston, Texas, United States of America. |
| Reference | Grass, S., Lichti, C.F., Townsend, R.R., Gross, J. and St. Geme III, J.W., 2010. The Haemophilus influenzae HMW1C protein is a glycosyltransferase that transfers hexose residues to asparagine sites in the HMW1 adhesin. PLoS pathogens, 6(5), p.e1000919. |
| Corresponding Author | St. Geme Joseph W. III |
| Contact | The Edward Mallinckrodt Department of Pediatrics and Department of Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Ave., Campus Box 8208, St Louis, MO 63110, USA. |
| Reference | Gross, J., Grass, S., Davis, A.E., Gilmore-Erdmann, P., Townsend, R.R. and Geme, J.W.S., 2008. The Haemophilus influenzae HMW1 adhesin is a glycoprotein with an unusual N-linked carbohydrate modification. Journal of Biological Chemistry, 283(38), pp.26010-26015. |
| Corresponding Author | St. Geme Joseph W. III |
| Contact | The Edward Mallinckrodt Department of Pediatrics and Department of Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Ave., Campus Box 8208, St Louis, MO 63110, USA. |
| Reference | Yeo, H.J., Yokoyama, T., Walkiewicz, K., Kim, Y., Grass, S. and Geme, J.W.S., 2007. The structure of the Haemophilus influenzae HMW1 pro-piece reveals a structural domain essential for bacterial two-partner secretion. Journal of Biological Chemistry, 282(42), pp.31076-31084. |
| Corresponding Author | Hye-Jeong Yeo |
| Contact | Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204, USA. |
| Reference | Grass, S., Buscher, A.Z., Swords, W.E., Apicella, M.A., Barenkamp, S.J., Ozchlewski, N. and St Geme III, J.W., 2003. The Haemophilus influenzae HMW1 adhesin is glycosylated in a process that requires HMW1C and phosphoglucomutase, an enzyme involved in lipooligosaccharide biosynthesis. Molecular microbiology, 48(3), pp.737-751. |
| Corresponding Author | St. Geme Joseph W. III |
| Contact | The Edward Mallinckrodt Department of Pediatrics and Department of Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Ave., Campus Box 8208, St Louis, MO 63110, USA. |
| Reference | Choi, K.J., Grass, S., Paek, S., St Geme, J.W., 3rd and Yeo, H.J. (2010) The Actinobacillus pleuropneumoniae HMW1C-like glycosyltransferase mediates N-linked glycosylation of the Haemophilus influenzae HMW1 adhesin. PLoS One, 5, e15888. [PubMed: 21209858] |
| Author | Choi, K.J., Grass, S., Paek, S., St Geme, J.W., 3rd and Yeo, H.J. |
| Research Group | Department of Biology and Biochemistry, University of Houston, Houston, Texas, United States of America. |
| Corresponding Author | Yeo, H.J. |
| Contact | Department of Biology and Biochemistry, University of Houston, Houston, Texas, United States of America. |
| Reference | Grass, S., Lichti, C.F., Townsend, R.R., Gross, J. and St Geme, J.W., 3rd. (2010) The Haemophilus influenzae HMW1C protein is a glycosyltransferase that transfers hexose residues to asparagine sites in the HMW1 adhesin. PLoS Pathog, 6, e1000919. [PubMed: 20523900] |
| Author | Grass, S., Lichti, C.F., Townsend, R.R., Gross, J. St Geme, J.W., 3rd. |
| Research Group | Department of Pediatrics, Duke University Medical Center, Durham, North Carolina, United States of America. |
| Corresponding Author | St Geme, J.W., 3rd. |
| Contact | Department of Pediatrics, Duke University Medical Center, Durham, North Carolina, United States of America. |
| Reference | Gross, J., Grass, S., Davis, A.E., Gilmore-Erdmann, P., Townsend, R.R. and St Geme, J.W., 3rd. (2008) The Haemophilus influenzae HMW1 adhesin is a glycoprotein with an unusual N-linked carbohydrate modification. J Biol Chem, 283, 26010-26015. [PubMed: 18621734] |
| Author | Gross, J., Grass, S., Davis, A.E., Gilmore-Erdmann, P., Townsend, R.R. and St Geme, J.W., 3rd. |
| Research Group | Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA. |
| Corresponding Author | St Geme, J.W., 3rd. |
| Contact | Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA. |
| Reference | Yeo, H.J., Yokoyama, T., Walkiewicz, K., Kim, Y., Grass, S. and Geme, J.W., 3rd. (2007) The structure of the Haemophilus influenzae HMW1 pro-piece reveals a structural domain essential for bacterial two-partner secretion. J Biol Chem, 282, 31076-31084. [PubMed: 17699157] |
| Author | Yeo, H.J., Yokoyama, T., Walkiewicz, K., Kim, Y., Grass, S. and Geme, J.W., |
| Research Group | Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204, USA. |
| Corresponding Author | Geme, J.W., 3rd. |
| Contact | Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204, USA |
| Reference | Grass, S., Buscher, A.Z., Swords, W.E., Apicella, M.A., Barenkamp, S.J., Ozchlewski, N. and St Geme, J.W., 3rd. (2003) The Haemophilus influenzae HMW1 adhesin is glycosylated in a process that requires HMW1C and phosphoglucomutase, an enzyme involved in lipooligosaccharide biosynthesis. Mol Microbiol, 48, 737-751. [PubMed: 12694618] |
| Author | Grass, S., Buscher, A.Z., Swords, W.E., Apicella, M.A., Barenkamp, S.J., Ozchlewski, N. St Geme, J.W., 3rd. |
| Research Group | The Edward Mallinckrodt Department of Pediatrics and Department of Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Ave., Campus Box 8208, St Louis, MO 63110, USA. |
| Corresponding Author | St Geme, J.W., 3rd. |
| Contact | The Edward Mallinckrodt Department of Pediatrics and Department of Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Ave., Campus Box 8208, St Louis, MO 63110, USA. |
