ProGP233 (Resuscitation promoting factor 2 (Rpf2))
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| ProGP ID | ProGP233 (Resuscitation promoting factor 2 (Rpf2)) |
| Validation Status | Uncharacterized |
| Organism Information | |
| Organism Name | Corynebacterium glutamicum (Brevibacterium flavum) ATCC 13032 |
| Domain | Bacteria |
| Classification | Phylum : Actinobacteria Class : Actinomycetia Orders : Corynebacteriales Family : Corynebacteriaceae Genus : Corynebacterium Species : glutamicum Strain : ATCC 13032 |
| Taxonomic ID (NCBI) | 1718 |
| Genome Information | |
| GenBank | BX927150.1 |
| EMBL | BX927150 |
| Gene Information | |
| Gene Name | rpf2 (cg1037) |
| NCBI Gene ID | 3342641 |
| GenBank Gene Sequence | NC_006958.1 |
| Protein Information | |
| Protein Name | Resuscitation promoting factor 2 (Rpf2) |
| UniProtKB/SwissProt ID | Q6M6N7 |
| NCBI RefSeq | WP_003858490.1 |
| EMBL-CDS | CAF19615.1 |
| UniProtKB Sequence | >tr|Q6M6N7|Q6M6N7_CORGL Putative RPF2, secreted protein OS=Corynebacterium glutamicum GN=cg1037 PE=4 SV=1 MAPHQKSRINRINSTRSVPLRLATGGVLATLLIGGVTAAATKKDIIVDVNGEQMSLVTMS GTVEGVLAQAGVELGDQDIVSPSLDSSISDEDTVTVRTAKQVALVVEGQIQNVTTTAVSV EDLLQEVGGITGADAVDADLSETIPESGLKVSVTKPKIISINDGGKVTYVSLAAQNVQEA LELRDIELGAQDRINVPLDQQLKNNAAIQIDRVDNTEITETVSFDAEPTYVDDPEAPAGD ETVVEEGAPGTKEVTRTVTTVNGQEESSTVINEVEITAAKPATISRGTKTVAANSVWDQL AQCESGGNWAINTGNGFSGGLQFHPQTWLAYGGGAFSGDASGASREQQISIAEKVQAAQG WGAWPACTASLGIR |
| Sequence length | 374 AA |
| Subcellular Location | Cell surface (secreted) |
| Function | Rpf2 plays a role in intercellular communication and growth stimulation of C. glutamicum. |
| Glycosylation Status | |
| Technique(s) used for Glycosylation Detection | DIG (digoxigenin) glycan detection using using anti-DIG antibody |
| Glycan Information | |
| Glycan Annotation | Galactose and mannose are the constituents of the oligosaccharide that were identified by GCMS. |
| Protein Glycosylation linked (PGL) gene(s) | |
| OST ProGT ID | ProGT14 |
| Accessory Gene(s)Progt ID | ProGT14.1 |
| Additional Comment | Rpf2 is the first example of a glycoprotein in C. glutamicum. Protein mannosyltransferase, pmt gene (cg1014) has been shown to glycosylate secreted proteins including Rpf2 by gene deletion studies. This gene has also been found to possess significant similarity to eukaryotic PMTs and an orthologue of M. tuberculosis, Rv1002c. |
| Literature | |
| Year of Identification | 2004 |
| Year of Identification Month Wise | 2004.11 |
| Reference | Mahne, M., Tauch, A., Pühler, A. and Kalinowski, J., 2006. The Corynebacterium glutamicum gene pmt encoding a glycosyltransferase related to eukaryotic protein-O-mannosyltransferases is essential for glycosylation of the resuscitation promoting factor (Rpf2) and other secreted proteins. FEMS microbiology letters, 259(2), pp.226-233. |
| Corresponding Author | Jörn Kalinowski |
| Contact | Institute for Genome Research, Center for Biotechnology, Bielefeld, Germany. |
| Reference | Hartmann, M., Barsch, A., Niehaus, K., Pühler, A., Tauch, A. and Kalinowski, J., 2004. The glycosylated cell surface protein Rpf2, containing a resuscitation-promoting factor motif, is involved in intercellular communication of Corynebacterium glutamicum. Archives of microbiology, 182(4), pp.299-312. |
| Corresponding Author | Jörn Kalinowski |
| Contact | Institute for Genome Research, Center for Biotechnology, Bielefeld, Germany. |