ProGP233 (Resuscitation promoting factor 2 (Rpf2))
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| ProGP ID | ProGP233 (Resuscitation promoting factor 2 (Rpf2)) |
| Validation Status | Uncharacterized |
| Organism Information | |
| Organism Name | Corynebacterium glutamicum (Brevibacterium flavum) ATCC 13032 |
| Domain | Bacteria |
| Classification | Family: Corynebacteriaceae Suborder: Corynebacterineae Order: Actinomycetales Subclass: Actinobacteridae Class: Actinobacteria Division or phylum: "Actinobacteria" |
| Taxonomic ID (NCBI) | 1718 |
| Genome Information | |
| GenBank | BX927150.1 |
| EMBL | BX927150 |
| Gene Information | |
| Gene Name | rpf2 (cg1037) |
| NCBI Gene ID | 3342641 |
| GenBank Gene Sequence | NC_006958.1 |
| Protein Information | |
| Protein Name | Resuscitation promoting factor 2 (Rpf2) |
| UniProtKB/SwissProt ID | Q6M6N7 |
| NCBI RefSeq | YP_225201.1 |
| EMBL-CDS | CAF19615.1 |
| UniProtKB Sequence | >tr|Q6M6N7|Q6M6N7_CORGL Putative RPF2, secreted protein OS=Corynebacterium glutamicum GN=cg1037 PE=4 SV=1 MAPHQKSRINRINSTRSVPLRLATGGVLATLLIGGVTAAATKKDIIVDVNGEQMSLVTMS GTVEGVLAQAGVELGDQDIVSPSLDSSISDEDTVTVRTAKQVALVVEGQIQNVTTTAVSV EDLLQEVGGITGADAVDADLSETIPESGLKVSVTKPKIISINDGGKVTYVSLAAQNVQEA LELRDIELGAQDRINVPLDQQLKNNAAIQIDRVDNTEITETVSFDAEPTYVDDPEAPAGD ETVVEEGAPGTKEVTRTVTTVNGQEESSTVINEVEITAAKPATISRGTKTVAANSVWDQL AQCESGGNWAINTGNGFSGGLQFHPQTWLAYGGGAFSGDASGASREQQISIAEKVQAAQG WGAWPACTASLGIR |
| Sequence length | 374 AA |
| Subcellular Location | Cell surface (secreted) |
| Function | Rpf2 plays a role in intercellular communication and growth stimulation of C. glutamicum. |
| Glycosylation Status | |
| Technique(s) used for Glycosylation Detection | DIG (digoxigenin) glycan detection using using anti-DIG antibody |
| Glycan Information | |
| Glycan Annotation | Galactose and mannose are the constituents of the oligosaccharide that were identified by GCMS. |
| Protein Glycosylation linked (PGL) gene(s) | |
| OST ProGT ID | ProGT14 (Pmt) |
| Literature | |
| Reference | Mahne, M., Tauch, A., Puhler, A. and Kalinowski, J. (2006) The Corynebacterium glutamicum gene pmt encoding a glycosyltransferase related to eukaryotic protein-O-mannosyltransferases is essential for glycosylation of the resuscitation promoting factor (Rpf2) and other secreted proteins. FEMS Microbiol Lett, 259, 226-233. [PubMed: 16734784] |
| Author | Kalinowski, J. |
| Research Group | Institute for Genome Research, Center for Biotechnology, Bielefeld, Germany. |
| Corresponding Author | Mahne, M., Tauch, A., Puhler, A. Kalinowski, J. |
| Contact | Institute for Genome Research, Center for Biotechnology, Bielefeld, Germany. |
| Reference | Hartmann, M., Barsch, A., Niehaus, K., Puhler, A., Tauch, A. and Kalinowski, J. (2004) The glycosylated cell surface protein Rpf2, containing a resuscitation-promoting factor motif, is involved in intercellular communication of Corynebacterium glutamicum. Arch Microbiol, 182, 299-312. [PubMed: 15480574] |
| Author | Hartmann, M., Barsch, A., Niehaus, K., Puhler, A., Tauch, A. Kalinowski, J. |
| Research Group | Department of Genetics, University of Bielefeld, Universitätsstrasse 25, Bielefeld, Germany |
| Corresponding Author | Kalinowski, J. |
| Contact | Department of Genetics, University of Bielefeld, Universitätsstrasse 25, Bielefeld, Germany |