| Technique(s) used for Glycosylation Detection | Mass shift detected on SDS-polyacrylamide gel and glycan identification by nano-LC-MS/MS |
| Technique(s) used for Glycosylated Residue(s) Detection | Nano-LC-MS/MS (nano-liquid chromatography-electrospray tandem mass spectrometry) and nano-ESI-feCID-MS/MS |
| Protein Glycosylation- Implication | The glycans on flagellin proteins are involved in the flagella assembly process. |
| Glycan Information |
| Glycan Annotation | Linkage: ?-GlcNAc-Asn.
Trisaccharide (779 Da) composed of ?-Manp NAcA6Thr-(1– 4)- ?-Glcp NAc3NAcA-(1–3)-?-Glcp NAc. The sugars are mannuronic acid with the attached threonine, diacetylated glucuronic acid, N-acetylglucosamine.
In a second version of this strain (M. voltae PS*), the glycan is modified with one additional residue (either 220 or 262 Da) linked to the terminal modified mannuronic acid. |
| BCSDB ID | 10182 |
| GlyTouCan | G47369PB |
| Technique(s) used for Glycan Identification | NanoLC–MS/MS analysis and NMR spectroscopy- COSY (correlated spectroscopy), TOCSY (total correlation spectroscopy), NOESY (nuclear Overhauser effect spectroscopy) spectra, and 1H-13C HMBC (heteronuclear multiple bond coherence) spectra. |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | AglB |
| OST ProGT ID | ProGT16 (AglB) |
| Literature |
| Reference | Chaban, B., Logan, S.M., Kelly, J.F. and Jarrell, K.F. (2009) AglC and AglK are involved in biosynthesis and attachment of diacetylated glucuronic acid to the N-glycan in Methanococcus voltae. J Bacteriol, 191, 187-195. [PubMed: 18978056] |
| Author | Chaban, B., Logan, S.M., Kelly, J.F. and Jarrell, K.F. |
| Research Group | Department of Microbiology and Immunology, Queens University, Kingston, Ontario, K7L 3N6, Canada. |
| Corresponding Author | Jarrell, K.F. |
| Contact | Department of Microbiology and Immunology, Queens University, Kingston, Ontario, K7L 3N6, Canada. |
| Reference | Shams-Eldin, H., Chaban, B., Niehus, S., Schwarz, R.T. and Jarrell, K.F. (2008) Identification of the archaeal alg7 gene homolog (encoding N-acetylglucosamine-1-phosphate transferase) of the N-linked glycosylation system by cross-domain complementation in Saccharomyces cerevisiae. J Bacteriol, 190, 2217-2220. [PubMed: 18178736] |
| Author | Shams-Eldin, H., Chaban, B., Niehus, S., Schwarz, R.T. and Jarrell, K.F |
| Research Group | Institute for Virology, AG Parasitologie BMFZ, Philipps-University Marburg, Hans-Meerwein-Str. 2, 35043 Marburg, Germany. |
| Corresponding Author | Jarrell, K.F. |
| Contact | Institute for Virology, AG Parasitologie BMFZ, Philipps-University Marburg, Hans-Meerwein-Str. 2, 35043 Marburg, Germany. |
| Reference | Chaban, B., Voisin, S., Kelly, J., Logan, S.M. and Jarrell, K.F. (2006) Identification of genes involved in the biosynthesis and attachment of Methanococcus voltae N-linked glycans: insight into N-linked glycosylation pathways in Archaea. Mol Microbiol, 61, 259-268. [PubMed: 16824110] |
| Author | Chaban, B., Voisin, S., Kelly, J., Logan, S.M. Jarrell, K.F. |
| Research Group | Department of Microbiology and Immunology, Queens University, Kingston, Ontario, K7L 3N6, Canada. |
| Corresponding Author | Jarrell, K.F. |
| Contact | Department of Microbiology and Immunology, Queens University, Kingston, Ontario, K7L 3N6, Canada. |
| Reference | Voisin, S., Houliston, R.S., Kelly, J., Brisson, J.R., Watson, D., Bardy, S.L., Jarrell, K.F. and Logan, S.M. (2005) Identification and characterization of the unique N-linked glycan common to the flagellins and S-layer glycoprotein of Methanococcus voltae. J Biol Chem, 280, 16586-16593. [PubMed: 15723834] |
| Author | Voisin, S., Houliston, R.S., Kelly, J., Brisson, J.R., Watson, D., Bardy, S.L., Jarrell, K.F. Logan, S.M. |
| Research Group | Institute for Biological Sciences, National Research Council, Ottawa, Ontario K1A OR6, Canada |
| Corresponding Author | Logan, S.M. |
| Contact | Institute for Biological Sciences, National Research Council, Ottawa, Ontario K1A OR6, Canada |
| Reference | Bayley, D.P. and Jarrell, K.F. (1999) Overexpression of Methanococcus voltae flagellin subunits in Escherichia coli and Pseudomonas aeruginosa: a source of archaeal preflagellin. J Bacteriol, 181, 4146-4153. [PubMed: 10400569] |
| Author | Bayley, D.P. Jarrell, K.F. |
| Research Group | Department of Microbiology and Immunology, Queens University, Kingston, Ontario, Canada K7L 3N6. |
| Corresponding Author | Jarrell, K.F. |
| Contact | Department of Microbiology and Immunology, Queens University, Kingston, Ontario, Canada K7L 3N6. |
