ProGP243 (Synthetic Glycopeptide)
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ProGP ID | ProGP243 (Synthetic Glycopeptide) |
Validation Status | Characterized |
Organism Information | |
Organism Name | Campylobacter jejuni NCTC 11168 |
Domain | Bacteria |
Classification | Phylum : Proteobacteria Class : Epsilonproteobacteria Orders : Campylobacterales Family : Campylobacteraceae Genus : Campylobacter Species : jejuni Strain : NCTC 11168 |
Taxonomic ID (NCBI) | 197 |
Genome Information | |
GenBank | AL111168.1 |
EMBL | AL111168 |
Organism Additional Information | Campylobacter jejuni is a microaerophilic, Gram-negative, human pathogen that is the major cause of bacterial food-borne diarrhoea (gastroenteritis). It is most frequently responsible for a form of post-infection neuromuscular paralysis known as Guillain Barre' syndrome. It also leads to an immunoproliferative small intestine disease that is a rare malignant lymphoma of the intestine. Motility is essential for pathogenicity. |
Protein Information | |
Protein Name | Synthetic Glycopeptide |
UniProtKB Sequence | KDFNVSKA |
Sequence length | 5 AA |
Function | NA |
Glycosylation Status | |
Glycosylation Type | N- (Asn) linked |
Experimentally Validated Glycosite(s) in Full Length Protein | N4 |
Experimentally Validated Glycosite(s ) in Mature Protein | N4 |
Glycosite(s) Annotated Protein Sequence | KDFN*(4)VSKA |
Technique(s) used for Glycosylation Detection | In vitro chemo enzymatic glycosylation,HPLC analysis of glycopeptides with reference to known glycopeptide HPLC profiles |
Technique(s) used for Glycosylated Residue(s) Detection | Not applicable |
Protein Glycosylation- Implication | Not applicable |
Glycan Information | |
Glycan Annotation | GalNAc-α1,3-bacillosamine. |
BCSDB ID | 20059 |
GlyTouCan | G82182GL |
Technique(s) used for Glycan Identification | NanoLC-MS/MS analysis and NMR spectroscopy-COSY (correlated spectroscopy), TOCSY (total correlation spectroscopy), NOESY (nuclear Overhauser effect spectroscopy) spectra, and 1H-13C HMBC (heteronuclear multiple bond coherence) spectra. |
Protein Glycosylation linked (PGL) gene(s) | |
OST Gene Name | PglB |
Additional Comment | Engineered glycopeptide. In vitro characterization of the oligosaccharyl transferase activity of PglB protein from membrane fraction of Campylobacter jejuni using a synthetic disaccharide glycan donor (GalNAc-β-1,3-bacillosamine-pyrophosphate-undecaprenyl) and a peptide acceptor substrate (KDFNVSKA). |
Literature | |
Year of Identification | 2005 |
Year of Identification Month Wise | 2005.4.29 |
Year of Validation | 2005 |
Reference | Glover, K.J., Weerapana, E., Numao, S. and Imperiali, B., 2005. Chemoenzymatic synthesis of glycopeptides with PglB, a bacterial oligosaccharyl transferase from Campylobacter jejuni. Chemistry & biology, 12(12), pp.1311-1316. |
Corresponding Author | Barbara Imperiali |
Contact | Department of Chemistry and Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA. |