| Technique(s) used for Glycosylation Detection | Immunoblotting, Trypsin digestion and mass spectrometry (Ion trap with CID) |
| Technique(s) used for Glycosylated Residue(s) Detection | MSMS (Ion trap with electron transfer dissociation (ETD)) |
| Protein Glycosylation- Implication | Pilin glycosylation has been shown to play a role in host-cell adhesion and virulence.
|
| Glycan Information |
| Glycan Annotation | Pentasaccharide (HexNAc-Hex-Hex-HexNAc-HexNAc). Orbitrap XL mass spectrometer using the higher-energy collisional fragmentation (HCD) mode suggests that pentasaccharide is linked through its terminal HexNAc residue to a 162.111-Da moiety via a phosphate bridge. |
| Technique(s) used for Glycan Identification | MS (ion trap with tandemMS CID and ETD) |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | PglA |
| OST ProGT ID | ProGT50 (PglA) |
| Literature |
| Reference | Egge-Jacobsen, W., Salomonsson, E.N., Aas, F.E., Forslund, A.L., Winther-Larsen, H.C., Maier, J., Macellaro, A., Kuoppa, K., Oyston, P.C., Titball, R.W. and Thomas, R.M., 2011. O-linked glycosylation of the PilA pilin protein of Francisella tularensis: identification of the endogenous protein-targeting oligosaccharyltransferase and characterization of the native oligosaccharide. Journal of bacteriology, 193(19), pp.5487-5497. |
| Corresponding Author | Wolfgang Egge-Jacobsen |
| Contact | Department of Molecular Biosciences, University of Oslo, 0316 Oslo, Norway. |
| Reference | Forslund, A.L., Kuoppa, K., Svensson, K., Salomonsson, E., Johansson, A., Byström, M., Oyston, P.C., Michell, S.L., Titball, R.W., Noppa, L. and Frithz‐Lindsten, E., 2006. Direct repeat‐mediated deletion of a type IV pilin gene results in major virulence attenuation of Francisella tularensis. Molecular microbiology, 59(6), pp.1818-1830. |
| Corresponding Author | Åke Forsberg |
| Contact | Department of Medical Countermeasures, Division of NBC-Defence, Swedish Defence Research Agency, Umea ,2.Department of Molecular Biology, Umeå University, SE-901 87 Umeå, Sweden. |
| Reference | Balonova, L., Hernychova, L., Mann, B.F., Link, M., Bilkova, Z., Novotny, M.V. and Stulik, J., 2010. Multimethodological approach to identification of glycoproteins from the proteome of Francisella tularensis, an intracellular microorganism. Journal of proteome research, 9(4), pp.1995-2005. |
| Corresponding Author | Lenka Hernychova |
| Contact | Institute of Molecular Pathology, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic |
| Reference | Egge-Jacobsen W, Salomonsson EN, Aas FE, Forslund AL, Winther-Larsen HC, Maier J, Macellaro A, Kuoppa K, Oyston PC, Titball RW, Thomas RM, Forsberg Å, Prior JL, Koomey M. (2011) O-linked glycosylation of the PilA pilin protein of Francisella tularensis: identification of the endogenous protein-targeting oligosaccharyltransferase and characterization of the native oligosaccharide. J Bacteriol., 193, 5487-97. [PMID: 21804002] |
| Author | Egge-Jacobsen W, Salomonsson EN, Aas FE, Forslund AL, Winther-Larsen HC, Maier J, Macellaro A, Kuoppa K, Oyston PC, Titball RW, Thomas RM, Forsberg Å, Prior JL, Koomey M |
| Research Group | Department of Molecular Biosciences, University of Oslo, 0316 Oslo, Norway. |
| Corresponding Author | Koomey M |
| Contact | Department of Molecular Biosciences, University of Oslo, 0316 Oslo, Norway. |
| Reference | Forslund AL, Kuoppa K, Svensson K, Salomonsson E, Johansson A, Byström M, Oyston PC, Michell SL, Titball RW, Noppa L, Frithz-Lindsten E, Forsman M, Forsberg A. (2006) Direct repeat-mediated deletion of a type IV pilin gene results in major virulence attenuation of Francisella tularensis. Mol Microbiol., 59, 1818-30. [PMID: 16553886] |
| Author | Forslund AL, Kuoppa K, Svensson K, Salomonsson E, Johansson A, Byström M, Oyston PC, Michell SL, Titball RW, Noppa L, Frithz-Lindsten E, Forsman M, Forsberg A |
| Research Group | Department of Medical Countermeasures, Division of NBC-Defence, Swedish Defence Research Agency, Umea |
| Corresponding Author | Forsberg A |
| Contact | Department of Medical Countermeasures, Division of NBC-Defence, Swedish Defence Research Agency, Umea |
| Reference | Balonova L, Hernychova L, Mann BF, Link M, Bilkova Z, Novotny MV, Stulik J. (2010) Multimethodological approach to identification of glycoproteins from the proteome of Francisella tularensis, an intracellular microorganism. J Proteome Res., 9, 1995-2005. [PMID: 20175567] |
| Author | Balonova L, Hernychova L, Mann BF, Link M, Bilkova Z, Novotny MV, Stulik J. |
| Research Group | Institute of Molecular Pathology, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic |
| Corresponding Author | Stulik J. |
| Contact | Institute of Molecular Pathology, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic |
