| Technique(s) used for Glycosylation Detection | Aberrant SDS-PAGE migration and glycan detection by MS |
| Technique(s) used for Glycosylated Residue(s) Detection | NanoLC-MS/MS (nano-liquid chromatography-electrospray tandem mass spectrometry) |
| Protein Glycosylation- Implication | Glycan is necessary for proper flagellar filament assembly and function. A minimal portion of the glycan (at least two sugars) is required to facilitate flagellar assembly and the presence of each additional sugar results in increased motility, as measured on semi-swarm plates. |
| Glycan Information |
| Glycan Annotation | Linkage: β-GalNAc-Asn.
N-linked tetrasaccharide (1036.4 Da) composed of N-acetylgalactosamine, di-acetylated glucuronic acid, an acetylated and acetamidino-modified mannuronic acid linked to threonine, and a novel terminal sugar: Sug-4-β-ManNAc3NAmA6Thr-4-β-GlcNAc3NAcA-3-β-GalNAc-Asn where Sug is a novel monosaccharide unit, (5S)-2-acetamido-2,4-dideoxy-5-O-methyl-α-L-erythro-hexos-5-ulo-1,5-pyranose. |
| BCSDB ID | 23737 |
| GlyTouCan | G54356DB |
| Technique(s) used for Glycan Identification | NanoLC–MS/MS analysis and 1H, 13C NMR spectroscopy |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | AglB/STT3 subunit |
| OST ProGT ID | ProGT13 (AglB) |
| Literature |
| Reference | Jarrell, K.F., Jones, G.M. and Nair, D.B., 2010. Biosynthesis and role of N-linked glycosylation in cell surface structures of archaea with a focus on flagella and S layers. International journal of microbiology, 2010. |
| Corresponding Author | Ken F. Jarrell |
| Contact | Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada. |
| Reference | Kelly, J., Logan, S.M., Jarrell, K.F., VanDyke, D.J. and Vinogradov, E., 2009. A novel N-linked flagellar glycan from Methanococcus maripaludis. Carbohydrate research, 344(5), pp.648-653. |
| Reference | VanDyke, D.J., Wu, J., Logan, S.M., Kelly, J.F., Mizuno, S., Aizawa, S.I. and Jarrell, K.F., 2009. Identification of genes involved in the assembly and attachment of a novel flagellin N‐linked tetrasaccharide important for motility in the archaeon Methanococcus maripaludis. Molecular microbiology, 72(3), pp.633-644. |
| Corresponding Author | Ken F. Jarrell |
| Contact | Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada. |
| Reference | VanDyke, D.J., Wu, J., Ng, S.Y., Kanbe, M., Chaban, B., Aizawa, S.I. and Jarrell, K.F., 2008. Identification of a putative acetyltransferase gene, MMP0350, which affects proper assembly of both flagella and pili in the archaeon Methanococcus maripaludis. Journal of bacteriology, 190(15), pp.5300-5307. |
| Corresponding Author | Ken F. Jarrell |
| Contact | Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada. |
| Reference | Jarrell, K.F., Jones, G.M. and Nair, D.B. (2010) Biosynthesis and role of N-linked glycosylation in cell surface structures of archaea with a focus on flagella and s layers. Int J Microbiol, 2010, 470138. [PubMed: 20976295] |
| Author | Jarrell, K.F., Jones, G.M. and Nair, D.B. |
| Research Group | Department of Microbiology and Immunology, Queens University, Kingston, ON, Canada |
| Corresponding Author | Nair, D.B. |
| Contact | Department of Microbiology and Immunology, Queens University, Kingston, ON, Canada |
| Reference | Kelly, J., Logan, S.M., Jarrell, K.F., VanDyke, D.J. and Vinogradov, E. (2009) A novel N-linked flagellar glycan from Methanococcus maripaludis. Carbohydr Res, 344, 648-653. [PubMed: 19203750] |
| Author | Kelly, J., Logan, S.M., Jarrell, K.F., VanDyke, D.J. and Vinogradov, E. |
| Research Group | Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada K1A 0R6. |
| Corresponding Author | Vinogradov, E. |
| Contact | Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada K1A 0R6. |
| Reference | Kelly, J., Logan, S.M., Jarrell, K.F., VanDyke, D.J. and Vinogradov, E. (2009) A novel N-linked flagellar glycan from Methanococcus maripaludis. Carbohydr Res, 344, 648-653. [PubMed: 19203750] |
| Author | Kelly, J., Logan, S.M., Jarrell, K.F., V yke, D.J. |
| Research Group | Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada K1A 0R6. |
| Corresponding Author | yke, D.J. |
| Contact | Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada K1A 0R6. |
| Reference | VanDyke, D.J., Wu, J., Logan, S.M., Kelly, J.F., Mizuno, S., Aizawa, S. and Jarrell, K.F. (2009) Identification of genes involved in the assembly and attachment of a novel flagellin N-linked tetrasaccharide important for motility in the archaeon Methanococcus maripaludis. Mol Microbiol, 72, 633-644. [PubMed: 19400781] |
| Author | VanDyke, D.J., Wu, J., Logan, S.M., Kelly, J.F., Mizuno, S., Aizawa, S. and Jarrell, K.F |
| Research Group | Department of Microbiology and Immunology, Queens University, Kingston, Ontario, Canada. |
| Corresponding Author | Jarrell, K.F |
| Contact | Department of Microbiology and Immunology, Queens University, Kingston, Ontario, Canada. |
| Reference | VanDyke, D.J., Wu, J., Ng, S.Y., Kanbe, M., Chaban, B., Aizawa, S. and Jarrell, K.F. (2008) Identification of a putative acetyltransferase gene, MMP0350, which affects proper assembly of both flagella and pili in the archaeon Methanococcus maripaludis. J Bacteriol, 190, 5300-5307. [PubMed: 18539748] |
| Author | VanDyke, D.J., Wu, J., Ng, S.Y., Kanbe, M., Chaban, B., Aizawa, S. and Jarrell, K.F. |
| Research Group | Department of Microbiology and Immunology, Queens University, Kingston, Ontario, Canada. |
| Corresponding Author | Jarrell, K.F. |
| Contact | Department of Microbiology and Immunology, Queens University, Kingston, Ontario, Canada. |
