ProGP261 (Flagellin (FlaA))

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ProGP ID ProGP261 (Flagellin (FlaA))
Validation Status Characterized
Organism Information
Organism NameClostridium botulinum F str. Langeland/ A str. FE9909ACS Alberta
Domain Bacteria
Classification Phylum : Firmicutes
Class : Clostridia
Orders : Eubacteriales
Family : Clostridiaceae
Genus : Clostridium
Species : botulinum
Strain : F str. Langeland/ A str. FE9909ACS Alberta
Taxonomic ID (NCBI) 441772
Genome Information
GenBank CP000728.1
EMBL CP000728
Organism Additional Information Clostridium botulinum is a Gram-positive spore-forming anaerobic bacterium that causes botulism. Botulism is a descending symmetrical paralysis caused by botulinum neurotoxin (BoNT). Regarding the way of its entrance into the body, it can lead to infant/adult intestinal toxaemia, wound botulism, or food-borne botulism.
Gene Information
Gene NameflaA (CLI_2781)
NCBI Gene ID 5404818
GenBank Gene Sequence NC_009699.1
Protein Information
Protein NameFlagellin (FlaA)
UniProtKB/SwissProt ID A7GGU9
NCBI RefSeq WP_003359201.1
EMBL-CDSABS41149.1
UniProtKB Sequence >tr|A7GGU9|A7GGU9_CLOBL Flagellin OS=Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) GN=CLI_2781 PE=4 SV=1 MIINHNLNAMNAHRQMAINTGSNGKAIEKLSSGLRINRAGDDAAGLAISEKMRGQIRGLN QASRNSQDSISLIQTAEGALNETHSILQRMRELAVQSANDTNVKVDRDNLQKEVAELQNE INRIASQTQFNTKNLLNGSAKSMVFQIGANKDQVMELTIAGMGTSALKVGSINIGSGKGA KSISGMIETIDNAINTVSGERAKLGANQNRLEHTIANLDNSAENLQAAESRIRDVDMAKE MMNFTKTNILTQAAQAMLAQANQAPQGVLQLLR
Sequence length 273 AA
Subcellular LocationSurface
Function Forms the filaments of bacterial flagella.
Glycosylation Status
Glycosylation Type O- (Ser) linked
Experimentally Validated Glycosite(s) in Full Length ProteinS126, S139, S142, S165, S171, S176, S182
Experimentally Validated Glycosite(s ) in Mature ProteinS126, S139, S142, S165, S171, S176, S182
Glycosite(s) Annotated Protein Sequence >tr|A7GGU9|A7GGU9_CLOBL Flagellin OS=Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) GN=CLI_2781 PE=4 SV=1 MIINHNLNAMNAHRQMAINTGSNGKAIEKLSSGLRINRAGDDAAGLAISEKMRGQIRGLN QASRNSQDSISLIQTAEGALNETHSILQRMRELAVQSANDTNVKVDRDNLQKEVAELQNE INRIAS*(126)QTQFNTKNLLNGS*(139)AKS*(142)MVFQIGANKDQVMELTIAGMGTS*(165)ALKVGS*(171)INIGS*(176)GKGA KS*(182)ISGMIETIDNAINTVSGERAKLGANQNRLEHTIANLDNSAENLQAAESRIRDVDMAKE MMNFTKTNILTQAAQAMLAQANQAPQGVLQLLR
Sequence Around Glycosites (21 AA) ELQNEINRIASQTQFNTKNLL
QFNTKNLLNGSAKSMVFQIGA
TKNLLNGSAKSMVFQIGANKD
MELTIAGMGTSALKVGSINIG
GMGTSALKVGSINIGSGKGAK
ALKVGSINIGSGKGAKSISGM
INIGSGKGAKSISGMIETIDN
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Technique(s) used for Glycosylation DetectionMass spectrometry
Technique(s) used for Glycosylated Residue(s) Detection Electron transfer dissociation (ETD) MS with and without collision-activated dissociation (CAD).
Glycan Information
Glycan Annotation A novel legionaminic acid derivative (417 Da), 7-acetamido-5-(N-methyl-glutam-4-yl)-amino-3,5,7,9-tetradeoxy-D-glycero-α-D-galacto-nonulosonic acid, (αLeg5GluNMe7Ac).
BCSDB ID 22742
GlyTouCan G29317XW
Technique(s) used for Glycan Identification Homonuclear and heteronuclear 1H, 13C-NMR spectra, selective TOCSY (total correlation spectroscopy), HSQC (heteronuclear single quantum coherence), HMBC (heteronuclear multiple bond coherence).
Protein Glycosylation linked (PGL) gene(s)
Additional CommentModification by legionaminic acid ('sialic acid-like' nonulosonate sugar) of Gram-positive flagellar proteins is reported for the first time.
Literature
Year of Identification2008
Year of Identification Month Wise2009.11.1
Year of Validation 2008
ReferenceTwine, S.M., Paul, C.J., Vinogradov, E., McNally, D.J., Brisson, J.R., Mullen, J.A., McMullin, D.R., Jarrell, H.C., Austin, J.W., Kelly, J.F. and Logan, S.M., 2008. Flagellar glycosylation in Clostridium botulinum. The FEBS journal, 275(17), pp.4428-4444.
Corresponding Author Susan M Logan
ContactNRC-Institute for Biological Sciences, Ottawa, Canada.