| Technique(s) used for Glycosylation Detection | Mass spectrometry |
| Technique(s) used for Glycosylated Residue(s) Detection | Electron transfer dissociation (ETD) MS with and without collision-activated dissociation (CAD). |
| Glycan Information |
| Glycan Annotation | A novel legionaminic acid derivative (417 Da), 7-acetamido-5-(N-methyl-glutam-4-yl)-amino-3,5,7,9-tetradeoxy-D-glycero-α-D-galacto-nonulosonic acid, (αLeg5GluNMe7Ac). |
| BCSDB ID | 22742 |
| GlyTouCan | G29317XW |
| Technique(s) used for Glycan Identification | Homonuclear and heteronuclear 1H, 13C-NMR spectra, selective TOCSY (total correlation spectroscopy), HSQC (heteronuclear single quantum coherence), HMBC (heteronuclear multiple bond coherence). |
| Literature |
| Year of Identification | 2008 |
| Year of Identification Month Wise | 2009.11.1 |
| Year of Validation | 2008 |
| Reference | Twine, S.M., Paul, C.J., Vinogradov, E., McNally, D.J., Brisson, J.R., Mullen, J.A., McMullin, D.R., Jarrell, H.C., Austin, J.W., Kelly, J.F. and Logan, S.M., 2008. Flagellar glycosylation in Clostridium botulinum. The FEBS journal, 275(17), pp.4428-4444. |
| Corresponding Author | Susan M Logan |
| Contact | NRC-Institute for Biological Sciences, Ottawa, Canada. |
| Reference | Twine, S.M., Paul, C.J., Vinogradov, E., McNally, D.J., Brisson, J.R., Mullen, J.A., McMullin, D.R., Jarrell, H.C., Austin, J.W., Kelly, J.F. et al. (2008) Flagellar glycosylation in Clostridium botulinum. FEBS J, 275, 4428-4444. [PubMed: 18671733] |
| Author | Twine, S.M., Paul, C.J., Vinogradov, E., McNally, D.J., Brisson, J.R., Mullen, J.A., McMullin, D.R., Jarrell, H.C., Austin, J.W., Kelly, J.F. et al. |
| Research Group | NRC-Institute for Biological Sciences, Ottawa, Canada. |
| Corresponding Author | Kelly, J.F |
| Contact | NRC-Institute for Biological Sciences, Ottawa, Canada. |
