| Technique(s) used for Glycosylation Detection | Intact mass analysis with QTOF2-MS (hybrid quadrupole time of flight mass spectrometry) |
| Technique(s) used for Glycosylated Residue(s) Detection | Electron transfer dissociation (ETD) MS |
| Protein Glycosylation- Implication | Glycosylation of the flagellin protein is required for proper assembly and consequent motility. |
| Glycan Information |
| Glycan Annotation | (398-Da glycan) O-linked HexNAc residue, to which a methylated aspartic acid is linked via a phosphate bond. Flagellins from a number of C. difficile isolates from more recent outbreaks are modified in O linkage with a heterogeneous glycan containing up to five monosaccharide residues with masses of 204 (HexNAc), 146 (deoxyhexose), 160 (methylated deoxyhexose), and 192 (heptose). |
| Technique(s) used for Glycan Identification | MS/MS (tandem mass spectrometry), nESI-feCID-MS/MS (nano-electrospray ionization?front-end collision-induced dissociation MS/MS) analyses |
| Protein Glycosylation linked (PGL) gene(s) |
| OST ProGT ID | ProGT32 (GT) |
| Literature |
| Reference | Twine, S.M., Reid, C.W., Aubry, A., McMullin, D.R., Fulton, K.M., Austin, J. and Logan, S.M., 2009. Motility and flagellar glycosylation in Clostridium difficile. Journal of bacteriology, 191(22), pp.7050-7062. |
| Corresponding Author | Susan M. Logan |
| Contact | NRC-Institute for Biological Sciences, Ottawa, Canada. |
| Reference | Hensbergen, P.J., de Ru, A.H., Friggen, A.H., Corver, J., Smits, W.K. and van Veelen, P.A., 2022. New insights into the type A glycan modification of Clostridioides difficile flagellar protein flagellin C by phosphoproteomics analysis. Journal of Biological Chemistry, 298(3). |
| Corresponding Author | Paul J. Hensbergen |
| Contact | Center for Proteomics and Metabolomics, and 2Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands |
| Reference | Twine, S.M., Reid, C.W., Aubry, A., McMullin, D.R., Fulton, K.M., Austin, J. and Logan, S.M. (2009) Motility and flagellar glycosylation in Clostridium difficile. J Bacteriol, 191, 7050-7062. [PubMed: 19749038] |
| Author | Twine, S.M., Paul, C.J., Vinogradov, E., McNally, D.J., Brisson, J.R., Mullen, J.A., McMullin, D.R., Jarrell, H.C., Austin, J.W., Kelly, J.F. et al. |
| Research Group | NRC-Institute for Biological Sciences, Ottawa, Canada. |
| Corresponding Author | Kelly, J.F |
| Contact | NRC-Institute for Biological Sciences, Ottawa, Canada. |
