ProGP300 (CycB)
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| ProGP ID | ProGP300 (CycB) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : Betaproteobacteria Orders : Neisseriales Family : Neisseriaceae Genus : Neisseria Species : gonorrhoeae Strain : strain ATCC 700825 / FA 1090 |
| Taxonomic ID (NCBI) | 242231 |
| Genome Information | |
| GenBank | AE004969.1 |
| EMBL | AE004969 |
| Organism Additional Information | Neisseria gonorrhoeae is the etiologic agent of the human disease gonorrhoea. It is equipped with a variety of adherence factors that help it in the colonization of diverse microenvironments in the human host. The property of phase and antigenic variation displayed by its type IV pilin enables it to avoid immune system thereby initiating the disease. |
| Gene Information | |
| Gene Name | NGO1328 |
| NCBI Gene ID | 3281927 |
| GenBank Gene Sequence | NC_002946.2 |
| Protein Information | |
| Protein Name | CycB |
| UniProtKB/SwissProt ID | Q5F759 |
| NCBI RefSeq | WP_002216672.1 |
| EMBL-CDS | AAW89978.1 |
| UniProtKB Sequence | >tr|Q5F759|Q5F759_NEIG1 Putative cytochrome OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) GN=NGO1328 PE=4 SV=1 MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVDATTEAATQTRIQPVGQ LTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNGDWAPRIAQGFDTLFQHAL NGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDEAAPADNAASGTASAPADSAA PAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPGIPGIGKKDDWAPRIKKGKETLH KHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSGAKF |
| Sequence length | 279 AA |
| Subcellular Location | Periplasm |
| Function | Cytochrome c5 |
| Glycosylation Status | |
| Glycosylation Type | O- (Ser) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | S173 |
| Experimentally Validated Glycosite(s ) in Mature Protein | S173 |
| Glycosite(s) Annotated Protein Sequence | >tr|Q5F759|Q5F759_NEIG1 Putative cytochrome OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) GN=NGO1328 PE=4 SV=1 MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVDATTEAATQTRIQPVGQ LTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNGDWAPRIAQGFDTLFQHAL NGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDEAAPADNAASGTAS*(173)APADSAA PAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPGIPGIGKKDDWAPRIKKGKETLH KHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSGAKF |
| Sequence Around Glycosites (21 AA) | PADNAASGTASAPADSAAPAE |
| Technique(s) used for Glycosylation Detection | Immunoblotting with antiglycan antibodies |
| Technique(s) used for Glycosylated Residue(s) Detection | Ion-trap MS (mass spectrometry) with electron transfer dissociation (ETD) |
| Glycan Information | |
| Glycan Annotation | O-AcHexDATDH (432 Da) and DATDH (229 Da) glycans. DATDH is 2,4 diacetamido-2,4,6 trideoxyhexose. |
| Technique(s) used for Glycan Identification | LC-ESI-MS/MS with CID (liquid chromatography-electrospray ionization-tandem mass spectrometry with collision-induced dissociation) |
| Protein Glycosylation linked (PGL) gene(s) | |
| OST Gene Name | PglO |
| OST ProGT ID | ProGT21 |
| Additional Comment | Sequone feature: S/T plus low complexity region. |
| Literature | |
| Year of Identification | 2009 |
| Year of Identification Month Wise | 2009.3.17 |
| Year of Validation | 2009 |
| Reference | Vik, Å., Aas, F.E., Anonsen, J.H., Bilsborough, S., Schneider, A., Egge-Jacobsen, W. and Koomey, M., 2009. Broad spectrum O-linked protein glycosylation in the human pathogen Neisseria gonorrhoeae. Proceedings of the National Academy of Sciences, 106(11), pp.4447-4452. |
| Corresponding Author | Wolfgang Egge-Jacobsen Michael Koomey |
| Contact | a.Department of Molecular Biosciences and b.Center for Molecular Biology and Neuroscience, University of Oslo, Oslo 0316, Norway |