ProGP300 (CycB)

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ProGP ID ProGP300 (CycB)
Validation Status Characterized
Organism Information
Organism NameNeisseria gonorrhoeae (strain ATCC 700825 / FA 1090)
Domain Bacteria
Classification Family: Neisseriaceae
Order: Neisseriales
Class: Betaproteobacteria
Division or phylum: "Proteobacteria"
Taxonomic ID (NCBI) 242231
Genome Information
GenBank AE004969.1
EMBL AE004969
Organism Additional Information Neisseria gonorrhoeae is the etiologic agent of the human disease gonorrhoea. It is equipped with a variety of adherence factors that help it in the colonization of diverse microenvironments in the human host. The property of phase and antigenic variation displayed by its type IV pilin enables it to avoid immune system thereby initiating the disease.
Gene Information
Gene NameNGO1328
NCBI Gene ID 3281927
GenBank Gene Sequence NC_002946
Protein Information
Protein NameCycB
UniProtKB/SwissProt ID Q5F759
NCBI RefSeq YP_208390.1
EMBL-CDSAAW89978.1
UniProtKB Sequence >tr|Q5F759|Q5F759_NEIG1 Putative cytochrome OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) GN=NGO1328 PE=4 SV=1 MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVDATTEAATQTRIQPVGQ LTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNGDWAPRIAQGFDTLFQHAL NGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDEAAPADNAASGTASAPADSAA PAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPGIPGIGKKDDWAPRIKKGKETLH KHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSGAKF
Sequence length 279 AA
Subcellular LocationPeriplasm
Function Cytochrome c5
Glycosylation Status
Glycosylation Type O- (Ser) linked
Experimentally Validated Glycosite(s) in Full Length ProteinS173
Experimentally Validated Glycosite(s ) in Mature ProteinS173
Glycosite(s) Annotated Protein Sequence >tr|Q5F759|Q5F759_NEIG1 Putative cytochrome OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) GN=NGO1328 PE=4 SV=1 MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVDATTEAATQTRIQPVGQ LTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNGDWAPRIAQGFDTLFQHAL NGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDEAAPADNAASGTAS*(173)APADSAA PAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPGIPGIGKKDDWAPRIKKGKETLH KHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSGAKF
Sequence Around Glycosites (21 AA) PADNAASGTASAPADSAAPAE
Technique(s) used for Glycosylation DetectionImmunoblotting with antiglycan antibodies
Technique(s) used for Glycosylated Residue(s) Detection Ion-trap MS (mass spectrometry) with electron transfer dissociation (ETD)
Glycan Information
Glycan Annotation O-AcHexDATDH (432 Da) and DATDH (229 Da) glycans. DATDH is 2,4 diacetamido-2,4,6 trideoxyhexose.
Technique(s) used for Glycan Identification LC-ESI-MS/MS with CID (liquid chromatography-electrospray ionization?tandem mass spectrometry with collision-induced dissociation)
Protein Glycosylation linked (PGL) gene(s)
OST Gene NamePglO
OST ProGT IDProGT21 (PglO)
Literature
ReferenceVik, Å., Aas, F.E., Anonsen, J.H., Bilsborough, S., Schneider, A., Egge-Jacobsen, W. and Koomey, M., 2009. Broad spectrum O-linked protein glycosylation in the human pathogen Neisseria gonorrhoeae. Proceedings of the National Academy of Sciences, 106(11), pp.4447-4452.
Corresponding Author Wolfgang Egge-Jacobsen
Michael Koomey
Contacta.Department of Molecular Biosciences and b.Center for Molecular Biology and Neuroscience, University of Oslo, Oslo 0316, Norway
ReferenceVik, A., Aas, F.E., Anonsen, J.H., Bilsborough, S., Schneider, A., Egge-Jacobsen, W. and Koomey, M. (2009) Broad spectrum O-linked protein glycosylation in the human pathogen Neisseria gonorrhoeae. Proc Natl Acad Sci U S A, 106, 4447-4452. [PubMed: 19251655]
AuthorVik, A., Aas, F.E., Anonsen, J.H., Bilsborough, S., Schneider, A., Egge-Jacobsen, W. and Koomey, M.
Research GroupDepartment of Molecular Biosciences and Center for Molecular Biology and Neuroscience, University of Oslo, Oslo 0316, Norway.
Corresponding Author Koomey, M.
ContactDepartment of Molecular Biosciences and Center for Molecular Biology and Neuroscience, University of Oslo, Oslo 0316, Norway.