
Technique(s) used for Glycosylation Detection | SBA (soybean agglutinin) affinity chromatography and detection of multiple bands on Western blot |
Technique(s) used for Glycosylated Residue(s) Detection | ESI/MS-MS (electrospray-ionization-tandem mass spectrometry) |
Protein Glycosylation- Implication | Glycosylation is not required for antigenicity. |
Glycan Information |
Glycan Annotation | Linkage: Bac-Asn. 1406 Da heptasaccharide composed of HexNAc-HexNAc-[Hex-]HexNAc-HexNAc-HexNAc-Bac-N-Asn-Xaa, where Bac is bacillosamine, 2,4-diacetamido-2,4,6-trideoxyglucopyranose. |
Technique(s) used for Glycan Identification | 1H, 13C NMR spectroscopy- one dimensional TOCSY (total correlation spectroscopy) and NOESY (nuclear Overhauser effect spectroscopy); HMBC (heteronuclear multiple bond coherence), HMQC (heteronuclear multiple quantum correlation) spectra. |
Protein Glycosylation linked (PGL) gene(s) |
OST Gene Name | PglB |
OST ProGT ID | ProGT9 (PglB) |
Literature |
Reference | Maita, N., Nyirenda, J., Igura, M., Kamishikiryo, J. and Kohda, D. (2010) Comparative structural biology of eubacterial and archaeal oligosaccharyltransferases. J Biol Chem, 285, 4941-4950. [PubMed: 20007322]
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Author | Maita, N., Nyirenda, J., Igura, M., Kamishikiryo, J. and Kohda, D. |
Research Group | Institute of Microbiology, Department of Biology, Swiss Federal Institute of Technology Zurich, ETH Hönggerberg, Switzerland. |
Corresponding Author | Kohda, D |
Contact | Institute of Microbiology, Department of Biology, Swiss Federal Institute of Technology Zurich, ETH Hönggerberg, Switzerland. |
Reference | Scott, N.E., Bogema, D.R., Connolly, A.M., Falconer, L., Djordjevic, S.P. and Cordwell, S.J. (2009) Mass spectrometric characterization of the surface-associated 42 kDa lipoprotein JlpA as a glycosylated antigen in strains of Campylobacter jejuni. J Proteome Res, 8, 4654-4664. [PubMed: 19689120] |
Author | Scott, N.E., Bogema, D.R., Connolly, A.M., Falconer, L., Djordjevic, S.P. Cordwell, S.J. |
Research Group | School of Molecular and Microbial Biosciences, The University of Sydney, Australia 2006, NSW. |
Corresponding Author | Cordwell, S.J. |
Contact | School of Molecular and Microbial Biosciences, The University of Sydney, Australia 2006, NSW. |
Reference | Szymanski, C.M., Burr, D.H. and Guerry, P. (2002) Campylobacter protein glycosylation affects host cell interactions. Infect Immun, 70, 2242-2244. [Pubmed: 11895996] |
Author | Szymanski, C.M., Burr, D.H. and Guerry, P. |
Research Group | Enteric Diseases Program, Naval Medical Research Center, Silver Spring, Marland 20910-7500, USA. |
Corresponding Author | Guerry, P |
Contact | Enteric Diseases Program, Naval Medical Research Center, Silver Spring, Marland 20910-7500, USA. |