ProGP339 (FTH_0069)
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| ProGP ID | ProGP339 (FTH_0069) |
| Validation Status | Uncharacterized |
| Organism Information | |
| Organism Name | Francisella tularensis ssp. holarctica strain FSC200 |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : Gammaproteobacteria Orders : Thiotrichales Family : Francisellaceae Genus : Francisella Species : tularensis Subspecies : holarctica Strain : FSC 200 |
| Taxonomic ID (NCBI) | 351581 |
| Genome Information | |
| GenBank | CP003862.1 |
| EMBL | CP003862.1 |
| Gene Information | |
| Gene Name | FTH_0069 |
| GenBank Gene Sequence | NC_008369.1 |
| Protein Information | |
| Protein Name | FTH_0069 |
| UniProtKB/SwissProt ID | A0A0B3VRI1 |
| NCBI RefSeq | WP_010032229.1 |
| EMBL-CDS | KIP31309.1 |
| UniProtKB Sequence | >tr|A0A0B3VRI1|A0A0B3VRI1_FRATU Uncharacterized protein OS=Francisella tularensis subsp. holarctica GN=CH66_1308 PE=4 SV=1 MRNIFKKTLLILLLSVIIASCGMLSNDQFVYLGHGENSPDYQLYYDKTQKLFVLIDKRNG CFQKDDTGTCLAFTLKQAREFREYVLAKMIEIDVRLAKDDYGNYAIEELRKAGVTTVNKP IKTKKVFATPVKQIVLDRKQQYHLVRKEYEIDSNLVAMVVNDKEGKKRIKVAYTVYFPGL EKEYSTELRPFIIDPEYLYTHMTIDAVHEAQFMQRDVMKSQTNVKKKVDDYLKDVVDSDK KDTGYTHQEVASSVAALDNDLITKADEQREDKETTLASGSSISTIAKKPINQESSGSTIQ TATKTASTTQVDSNDTPKTTLASDNK |
| Sequence length | 326 AA |
| Subcellular Location | Membrane |
| Protein Structure | |
| Protein Additional Information | Hypothetical protein |
| Glycosylation Status | |
| Glycosylation Type | Not identified but probably O- (Ser/Thr) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | Not identified |
| Technique(s) used for Glycosylation Detection | Hydrazide labeling and lectin blotting |
| Glycan Information | |
| Glycan Annotation | Hexasaccharideof 1156 Da and identified sequence is X-P-HexNAc-HexNAc-Hex-Hex-HexNAc, where X denotes the unknown monosaccharide containing a phosphate group. |
| Technique(s) used for Glycan Identification | In gel trypsin digestion and nano-scale HPLC coupled on-line with an LTQ-FT-ICR mass spectrometer using a stepped orifice voltage scan |
| Literature | |
| Year of Identification | 2010 |
| Year of Identification Month Wise | 2010.4.5 |
| Year of Validation | 2012 |
| Reference | Balonova, L., Hernychova, L., Mann, B.F., Link, M., Bilkova, Z., Novotny, M.V. and Stulik, J., 2010. Multimethodological approach to identification of glycoproteins from the proteome of Francisella tularensis, an intracellular microorganism. Journal of proteome research, 9(4), pp.1995-2005. |
| Corresponding Author | Lenka Hernychova |
| Contact | Institute of Molecular Pathology, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic |
| Reference | Balonova, L., Mann, B.F., Cerveny, L., Alley, W.R., Chovancova, E., Forslund, A.L., Salomonsson, E.N., Forsberg, Å., Damborsky, J., Novotny, M.V. and Hernychova, L., 2012. Characterization of protein glycosylation in Francisella tularensis subsp. holarctica: identification of a novel glycosylated lipoprotein required for virulence. Molecular & Cellular Proteomics, 11(7), pp.M111-015016. |
| Corresponding Author | Lenka Hernychova |
| Contact | Institute of Molecular Pathology, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic |