| Technique(s) used for Glycosylation Detection | Glycan identification by nano-LC-MS/MS |
| Technique(s) used for Glycosylated Residue(s) Detection | In-gel tryptic digestion and nano-LC-ES-MS/MS (nano-liquid chromatography-electrospray tandem mass spectrometry) |
| Protein Glycosylation- Implication | Glycosylation of S layer protein might contribute towards increased stability and half life of S layer structural proteins in extreme thermoacidic enviornments. |
| Glycan Information |
| Glycan Annotation | Linkage: ?-GlcNAc-Asn.
N-linked hexasaccharide units - two residues each of Man and GlcNAc (one trisubstituted) and one residue each of Glc and 6-deoxy-6-sulfoglucose (6-sulfoquinovose, QuiS - a rare acidic sugar). ?-D-Glcp-(1?4)-?-D-Quip(6--SO3-)-(1?3)-[?-D-Manp(1?4), ?-D-Manp(1?6)] ?-D-GlcpNAc-(1?4)-?-D-GlcpNAc-(1?Asn....
Microheterogeneity is observed as a family of glycans modify each of the glycosylation sites. The glycans are linked via a chitobiose core. |
| BCSDB ID | 5950 |
| GlyTouCan | G92308TW |
| Technique(s) used for Glycan Identification | GC-MS of trimethylsilyl (TMS) methyl glycoside derivatives and nano-LC-ESMS analysis |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | Conserved membrane protein |
| OST ProGT ID | ProGT51 (AglB) |
| Literature |
| Reference | Peyfoon, E., Meyer, B., Hitchen, P.G., Panico, M., Morris, H.R., Haslam, S.M., Albers, S.V. and Dell, A. (2010) The S-layer glycoprotein of the crenarchaeote Sulfolobus acidocaldarius is glycosylated at multiple sites with chitobiose-linked N-glycans. Archaea, 2010. [PubMed: 20936123] |
| Author | Peyfoon, E., Meyer, B., Hitchen, P.G., Panico, M., Morris, H.R., Haslam, S.M., Albers, S.V. and Dell, A. |
| Research Group | Division of Molecular Biosciences, Faculty of Natural Sciences, Imperial College London, London, UK. |
| Corresponding Author | Dell, A. |
| Contact | Division of Molecular Biosciences, Faculty of Natural Sciences, Imperial College London, London, UK. |
| Reference | Veith, A., Klingl, A., Zolghadr, B., Lauber, K., Mentele, R., Lottspeich, F., Rachel, R., Albers, S.V. and Kletzin, A. (2009) Acidianus, Sulfolobus and Metallosphaera surface layers: structure, composition and gene expression. Mol Microbiol, 73, 58-72. [Pubmed 19522740] |
| Author | Veith, A., Klingl, A., Zolghadr, B., Lauber, K., Mentele, R., Lottspeich, F., Rachel, R., Albers, S.V. and Kletzin, A. |
| Research Group | Institute of Microbiology and Genetics, Technical University of Darmstadt, Schnittpahnstrasse 10, 64287 Darmstadt, Germany. |
| Corresponding Author | Kletzin, A. |
| Contact | Institute of Microbiology and Genetics, Technical University of Darmstadt, Schnittpahnstrasse 10, 64287 Darmstadt, Germany. |
| Reference | Grogan, D.W. (1996) Organization and interactions of cell envelope proteins of the extreme thermoacidophile Sulfolobus acidocaldarius. Can J Microbiol, 42, 1163-1171. |
| Author | Grogan, D.W. |
| Research Group | Department of Microbiology and Molecular Genetics, 5304 Life Sciences Building, University of California, Los Angeles, CA 90024-1489, U.S.A, and Department of Biological Sciences, University of Cincinnati, P.O. Box 21000t5, Cincinnati, Ohio 45221-0006, U.S.A. |
| Corresponding Author | Grogan, D.W. |
| Contact | Department of Microbiology and Molecular Genetics, 5304 Life Sciences Building, University of California, Los Angeles, CA 90024-1489, U.S.A, and Department of Biological Sciences, University of Cincinnati, P.O. Box 21000t5, Cincinnati, Ohio 45221-0006, U.S.A. |
| Reference | Grogan, D.W. (1989) Phenotypic characterization of the archaebacterial genus Sulfolobus: comparison of five wild-type strains. J Bacteriol, 171, 6710-6719. [PubMed: 2512283] |
| Author | Grogan, D.W. |
| Research Group | Max Planck Institute for Biochemistry, Martinsried, Federal Republic of Germany. |
| Corresponding Author | Grogan, D.W. |
| Contact | Max Planck Institute for Biochemistry, Martinsried, Federal Republic of Germany. |
