| Technique(s) used for Glycosylation Detection | Slower migration on SDS-PAGE than its predicted mass and N-glycosidase treatment |
| Technique(s) used for Glycosylated Residue(s) Detection | Multi-site-directed mutagenesis (mostly Asn→Gln) |
| Protein Glycosylation- Implication | Carbohydrate chains either distort the protein structure or produce steric hindrance of the active site resulting in an inactive form of the secreted protein. Secretion of underglycosylated protein was better when expressed in mammalian cells compared to fully glycosylated protein. |
| Literature |
| Year of Identification | 2010 |
| Year of Identification Month Wise | 2010.10.29 |
| Year of Validation | 2010 |
| Reference | Muir, E.M., Fyfe, I., Gardiner, S., Li, L., Warren, P., Fawcett, J.W., Keynes, R.J. and Rogers, J.H., 2010. Modification of N-glycosylation sites allows secretion of bacterial chondroitinase ABC from mammalian cells. Journal of biotechnology, 145(2), pp.103-110. |
| Corresponding Author | John H. Rogers |
| Contact | Department of Physiology Development and Neuroscience, University of Cambridge, Downing St., Cambridge CB2 3EG, UK. |
| Reference | Huang, W., Lunin, V., Li, Y., Suzuki, S., Sugiura, N., Miyazono, H. and Cygler, M., 2003. Crystal structure of Proteus vulgaris chondroitin sulfate ABC lyase I at 1.9 Å resolution. Journal of molecular biology, 328(3), pp.623-634. |
| Corresponding Author | Miroslaw Cygler |
| Contact | Biotechnology Research Institute, National Research Council of Canada, 6100 Royalmount Avenue, Montréal, Québec, Canada H4P 2R2. |
| Reference | Muir, E.M., Fyfe, I., Gardiner, S., Li, L., Warren, P., Fawcett, J.W., Keynes, R.J. and Rogers, J.H. (2010) Modification of N-glycosylation sites allows secretion of bacterial chondroitinase ABC from mammalian cells. J Biotechnol, 145, 103-110. [PubMed: 19900493] |
| Author | Muir, E.M., Fyfe, I., Gardiner, S., Li, L., Warren, P., Fawcett, J.W., Keynes, R.J. and Rogers, J.H. |
| Research Group | Department of Physiology Development and Neuroscience, University of Cambridge, Downing St., Cambridge CB2 3EG, UK. |
| Corresponding Author | Rogers, J.H. |
| Contact | Department of Physiology Development and Neuroscience, University of Cambridge, Downing St., Cambridge CB2 3EG, UK. |
| Reference | Huang, W., Lunin, V.V., Li, Y., Suzuki, S., Sugiura, N., Miyazono, H. and Cygler, M. (2003) Crystal structure of Proteus vulgaris chondroitin sulfate ABC lyase I at 1.9A resolution. J Mol Biol, 328, 623-634. [PubMed: 12706721] |
| Author | Huang, W., Lunin, V.V., Li, Y., Suzuki, S., Sugiura, N., Miyazono, H. Cygler, M. |
| Research Group | Biotechnology Research Institute, National Research Council of Canada, 6100 Royalmount Avenue, Montréal, Québec, Canada |
| Corresponding Author | Cygler, M. |
| Contact | Biotechnology Research Institute, National Research Council of Canada, 6100 Royalmount Avenue, Montréal, Québec, Canada |
